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Dynamics and consequences of spliceosome E complex formation
The spliceosome must identify the correct splice sites (SS) and branchsite (BS) used during splicing. E complex is the earliest spliceosome precursor in which the 5' SS and BS are defined. Definition occurs by U1 small nuclear ribonucleoprotein (snRNP) binding the 5' SS and recognition of...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5779234/ https://www.ncbi.nlm.nih.gov/pubmed/28829039 http://dx.doi.org/10.7554/eLife.27592 |
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author | Larson, Joshua Donald Hoskins, Aaron A |
author_facet | Larson, Joshua Donald Hoskins, Aaron A |
author_sort | Larson, Joshua Donald |
collection | PubMed |
description | The spliceosome must identify the correct splice sites (SS) and branchsite (BS) used during splicing. E complex is the earliest spliceosome precursor in which the 5' SS and BS are defined. Definition occurs by U1 small nuclear ribonucleoprotein (snRNP) binding the 5' SS and recognition of the BS by the E complex protein (ECP) branchpoint bridging protein (BBP). We have used single molecule fluorescence to study Saccharomyces cerevisiae U1 and BBP interactions with RNAs. E complex is dynamic and permits frequent redefinition of the 5' SS and BS. BBP influences U1 binding at the 5' SS by promoting long-lived complex formation. ECPs facilitate U1 association with RNAs with weak 5' SS and prevent U1 accumulation on RNAs containing hyperstabilized 5' SS. The data reveal a mechanism for how U1 binds the 5' SS and suggest that E complex harnesses this mechanism to stimulate recruitment and retention of U1 on introns. |
format | Online Article Text |
id | pubmed-5779234 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-57792342018-01-25 Dynamics and consequences of spliceosome E complex formation Larson, Joshua Donald Hoskins, Aaron A eLife Biochemistry and Chemical Biology The spliceosome must identify the correct splice sites (SS) and branchsite (BS) used during splicing. E complex is the earliest spliceosome precursor in which the 5' SS and BS are defined. Definition occurs by U1 small nuclear ribonucleoprotein (snRNP) binding the 5' SS and recognition of the BS by the E complex protein (ECP) branchpoint bridging protein (BBP). We have used single molecule fluorescence to study Saccharomyces cerevisiae U1 and BBP interactions with RNAs. E complex is dynamic and permits frequent redefinition of the 5' SS and BS. BBP influences U1 binding at the 5' SS by promoting long-lived complex formation. ECPs facilitate U1 association with RNAs with weak 5' SS and prevent U1 accumulation on RNAs containing hyperstabilized 5' SS. The data reveal a mechanism for how U1 binds the 5' SS and suggest that E complex harnesses this mechanism to stimulate recruitment and retention of U1 on introns. eLife Sciences Publications, Ltd 2017-08-22 /pmc/articles/PMC5779234/ /pubmed/28829039 http://dx.doi.org/10.7554/eLife.27592 Text en © 2017, Larson et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Biochemistry and Chemical Biology Larson, Joshua Donald Hoskins, Aaron A Dynamics and consequences of spliceosome E complex formation |
title | Dynamics and consequences of spliceosome E complex formation |
title_full | Dynamics and consequences of spliceosome E complex formation |
title_fullStr | Dynamics and consequences of spliceosome E complex formation |
title_full_unstemmed | Dynamics and consequences of spliceosome E complex formation |
title_short | Dynamics and consequences of spliceosome E complex formation |
title_sort | dynamics and consequences of spliceosome e complex formation |
topic | Biochemistry and Chemical Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5779234/ https://www.ncbi.nlm.nih.gov/pubmed/28829039 http://dx.doi.org/10.7554/eLife.27592 |
work_keys_str_mv | AT larsonjoshuadonald dynamicsandconsequencesofspliceosomeecomplexformation AT hoskinsaarona dynamicsandconsequencesofspliceosomeecomplexformation |