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The expression of glycerol facilitators from various yeast species improves growth on glycerol of Saccharomyces cerevisiae

Glycerol is an abundant by-product during biodiesel production and additionally has several assets compared to sugars when used as a carbon source for growing microorganisms in the context of biotechnological applications. However, most strains of the platform production organism Saccharomyces cerev...

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Autores principales: Klein, Mathias, Islam, Zia-ul, Knudsen, Peter Boldsen, Carrillo, Martina, Swinnen, Steve, Workman, Mhairi, Nevoigt, Elke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5779717/
https://www.ncbi.nlm.nih.gov/pubmed/29468128
http://dx.doi.org/10.1016/j.meteno.2016.09.001
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author Klein, Mathias
Islam, Zia-ul
Knudsen, Peter Boldsen
Carrillo, Martina
Swinnen, Steve
Workman, Mhairi
Nevoigt, Elke
author_facet Klein, Mathias
Islam, Zia-ul
Knudsen, Peter Boldsen
Carrillo, Martina
Swinnen, Steve
Workman, Mhairi
Nevoigt, Elke
author_sort Klein, Mathias
collection PubMed
description Glycerol is an abundant by-product during biodiesel production and additionally has several assets compared to sugars when used as a carbon source for growing microorganisms in the context of biotechnological applications. However, most strains of the platform production organism Saccharomyces cerevisiae grow poorly in synthetic glycerol medium. It has been hypothesized that the uptake of glycerol could be a major bottleneck for the utilization of glycerol in S. cerevisiae. This species exclusively relies on an active transport system for glycerol uptake. This work demonstrates that the expression of predicted glycerol facilitators (Fps1 homologues) from superior glycerol-utilizing yeast species such as Pachysolen tannophilus, Komagataella pastoris, Yarrowia lipolytica and Cyberlindnera jadinii significantly improves the growth performance on glycerol of the previously selected glycerol-consuming S. cerevisiae wild-type strain (CBS 6412-13A). The maximum specific growth rate increased from 0.13 up to 0.18 h(−1) and a biomass yield coefficient of 0.56 g(DW)/g(glycerol) was observed. These results pave the way for exploiting the assets of glycerol in the production of fuels, chemicals and pharmaceuticals based on baker's yeast.
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spelling pubmed-57797172018-02-21 The expression of glycerol facilitators from various yeast species improves growth on glycerol of Saccharomyces cerevisiae Klein, Mathias Islam, Zia-ul Knudsen, Peter Boldsen Carrillo, Martina Swinnen, Steve Workman, Mhairi Nevoigt, Elke Metab Eng Commun Article Glycerol is an abundant by-product during biodiesel production and additionally has several assets compared to sugars when used as a carbon source for growing microorganisms in the context of biotechnological applications. However, most strains of the platform production organism Saccharomyces cerevisiae grow poorly in synthetic glycerol medium. It has been hypothesized that the uptake of glycerol could be a major bottleneck for the utilization of glycerol in S. cerevisiae. This species exclusively relies on an active transport system for glycerol uptake. This work demonstrates that the expression of predicted glycerol facilitators (Fps1 homologues) from superior glycerol-utilizing yeast species such as Pachysolen tannophilus, Komagataella pastoris, Yarrowia lipolytica and Cyberlindnera jadinii significantly improves the growth performance on glycerol of the previously selected glycerol-consuming S. cerevisiae wild-type strain (CBS 6412-13A). The maximum specific growth rate increased from 0.13 up to 0.18 h(−1) and a biomass yield coefficient of 0.56 g(DW)/g(glycerol) was observed. These results pave the way for exploiting the assets of glycerol in the production of fuels, chemicals and pharmaceuticals based on baker's yeast. Elsevier 2016-09-29 /pmc/articles/PMC5779717/ /pubmed/29468128 http://dx.doi.org/10.1016/j.meteno.2016.09.001 Text en © 2016 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Klein, Mathias
Islam, Zia-ul
Knudsen, Peter Boldsen
Carrillo, Martina
Swinnen, Steve
Workman, Mhairi
Nevoigt, Elke
The expression of glycerol facilitators from various yeast species improves growth on glycerol of Saccharomyces cerevisiae
title The expression of glycerol facilitators from various yeast species improves growth on glycerol of Saccharomyces cerevisiae
title_full The expression of glycerol facilitators from various yeast species improves growth on glycerol of Saccharomyces cerevisiae
title_fullStr The expression of glycerol facilitators from various yeast species improves growth on glycerol of Saccharomyces cerevisiae
title_full_unstemmed The expression of glycerol facilitators from various yeast species improves growth on glycerol of Saccharomyces cerevisiae
title_short The expression of glycerol facilitators from various yeast species improves growth on glycerol of Saccharomyces cerevisiae
title_sort expression of glycerol facilitators from various yeast species improves growth on glycerol of saccharomyces cerevisiae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5779717/
https://www.ncbi.nlm.nih.gov/pubmed/29468128
http://dx.doi.org/10.1016/j.meteno.2016.09.001
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