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Identification of differentially expressed genes in Budd-Chiari syndrome by RNA-sequencing

Budd-Chiari syndrome (BCS) is an uncommon disease characterized by the occlusion or obstruction of hepatic venous outflow. The mechanism of BCS is still unclear and there are no accurate and effective diagnostic or therapeutic tools. In the present study, blood samples from BCS patients and healthy...

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Autores principales: Yang, Bin, Qu, Dong, Zhao, An-Li, Li, Yu, Meng, Ran-Ran, Yu, Ji-Xiang, Gao, Peng, Lin, Hua Peng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5779883/
https://www.ncbi.nlm.nih.gov/pubmed/28983615
http://dx.doi.org/10.3892/mmr.2017.7621
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author Yang, Bin
Qu, Dong
Zhao, An-Li
Li, Yu
Meng, Ran-Ran
Yu, Ji-Xiang
Gao, Peng
Lin, Hua Peng
author_facet Yang, Bin
Qu, Dong
Zhao, An-Li
Li, Yu
Meng, Ran-Ran
Yu, Ji-Xiang
Gao, Peng
Lin, Hua Peng
author_sort Yang, Bin
collection PubMed
description Budd-Chiari syndrome (BCS) is an uncommon disease characterized by the occlusion or obstruction of hepatic venous outflow. The mechanism of BCS is still unclear and there are no accurate and effective diagnostic or therapeutic tools. In the present study, blood samples from BCS patients and healthy controls were used for RNA-sequencing. The differentially expressed genes (DEGs) in BCS patients compared with healthy controls were identified. Gene Ontology (GO) enrichment analysis, Kyoto Encyclopedia of Genes and Genomes pathway analysis and Protein-Protein Interaction (PPI) networks construction were performed for DEGs. A total of 405 DEGs including 317 upregulated and 88 downregulated DEGs were identified. The cytosol was the most significantly enriched GO term and the proteasome was also identified as significant enriched pathway. According to the PPI network of 30 DEGs (18 upregulated and 12 downregulated DEGs), synuclein α, tubulin β-2A class IIa and zinc finger protein Gfi-1b (GFIIB) were the three most significant hub proteins. In conclusion, several DEGs including secreted protein acidic and cysteine rich, lipocalin-2, GFI1B and proteasome-associated DEGs may be associated with the pathological process of BCS. These results can provide novel clues for the pathogenesis and provide novel diagnostic and therapeutic strategies for BCS.
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spelling pubmed-57798832018-02-12 Identification of differentially expressed genes in Budd-Chiari syndrome by RNA-sequencing Yang, Bin Qu, Dong Zhao, An-Li Li, Yu Meng, Ran-Ran Yu, Ji-Xiang Gao, Peng Lin, Hua Peng Mol Med Rep Articles Budd-Chiari syndrome (BCS) is an uncommon disease characterized by the occlusion or obstruction of hepatic venous outflow. The mechanism of BCS is still unclear and there are no accurate and effective diagnostic or therapeutic tools. In the present study, blood samples from BCS patients and healthy controls were used for RNA-sequencing. The differentially expressed genes (DEGs) in BCS patients compared with healthy controls were identified. Gene Ontology (GO) enrichment analysis, Kyoto Encyclopedia of Genes and Genomes pathway analysis and Protein-Protein Interaction (PPI) networks construction were performed for DEGs. A total of 405 DEGs including 317 upregulated and 88 downregulated DEGs were identified. The cytosol was the most significantly enriched GO term and the proteasome was also identified as significant enriched pathway. According to the PPI network of 30 DEGs (18 upregulated and 12 downregulated DEGs), synuclein α, tubulin β-2A class IIa and zinc finger protein Gfi-1b (GFIIB) were the three most significant hub proteins. In conclusion, several DEGs including secreted protein acidic and cysteine rich, lipocalin-2, GFI1B and proteasome-associated DEGs may be associated with the pathological process of BCS. These results can provide novel clues for the pathogenesis and provide novel diagnostic and therapeutic strategies for BCS. D.A. Spandidos 2017-12 2017-09-26 /pmc/articles/PMC5779883/ /pubmed/28983615 http://dx.doi.org/10.3892/mmr.2017.7621 Text en Copyright: © Yang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Yang, Bin
Qu, Dong
Zhao, An-Li
Li, Yu
Meng, Ran-Ran
Yu, Ji-Xiang
Gao, Peng
Lin, Hua Peng
Identification of differentially expressed genes in Budd-Chiari syndrome by RNA-sequencing
title Identification of differentially expressed genes in Budd-Chiari syndrome by RNA-sequencing
title_full Identification of differentially expressed genes in Budd-Chiari syndrome by RNA-sequencing
title_fullStr Identification of differentially expressed genes in Budd-Chiari syndrome by RNA-sequencing
title_full_unstemmed Identification of differentially expressed genes in Budd-Chiari syndrome by RNA-sequencing
title_short Identification of differentially expressed genes in Budd-Chiari syndrome by RNA-sequencing
title_sort identification of differentially expressed genes in budd-chiari syndrome by rna-sequencing
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5779883/
https://www.ncbi.nlm.nih.gov/pubmed/28983615
http://dx.doi.org/10.3892/mmr.2017.7621
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