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SSRP1 silencing inhibits the proliferation and malignancy of human glioma cells via the MAPK signaling pathway

Structure-specific recognition protein 1 (SSRP1) has been considered as a potential biomarker, since aberrant high expression of SSRP1 has been detected in numerous malignant tumors. However, the correlation between the expression level of SSRP1 and glioma remains unclear. The present study attempte...

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Autores principales: Liao, Jianming, Tao, Xiang, Ding, Qianshan, Liu, Junhui, Yang, Xue, Yuan, Fan-En, Yang, Ji-An, Liu, Baohui, Xiang, Guo-An, Chen, Qianxue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5780019/
https://www.ncbi.nlm.nih.gov/pubmed/29048646
http://dx.doi.org/10.3892/or.2017.5982
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author Liao, Jianming
Tao, Xiang
Ding, Qianshan
Liu, Junhui
Yang, Xue
Yuan, Fan-En
Yang, Ji-An
Liu, Baohui
Xiang, Guo-An
Chen, Qianxue
author_facet Liao, Jianming
Tao, Xiang
Ding, Qianshan
Liu, Junhui
Yang, Xue
Yuan, Fan-En
Yang, Ji-An
Liu, Baohui
Xiang, Guo-An
Chen, Qianxue
author_sort Liao, Jianming
collection PubMed
description Structure-specific recognition protein 1 (SSRP1) has been considered as a potential biomarker, since aberrant high expression of SSRP1 has been detected in numerous malignant tumors. However, the correlation between the expression level of SSRP1 and glioma remains unclear. The present study attempted to investigate the role of SSRP1 in the pathogenesis of glioma. In the present study, our data revealed that SSRP1 overexpression was detected in glioma tissues at both the mRNA and protein levels using quantitative real-time RT-PCR and immunohistochemical analysis. We also demonstrated that the upregulated expression of SSRP1 was correlated with the World Health Organization (WHO) grade of glioma. The knockdown of SSRP1 by siRNA not only resulted in the inhibition of cell proliferation, but also significantly inhibited glioma cell migration and invasion. Mechanistic analyses revealed that SSRP1 depletion suppressed the activity of the phosphorylation of the MAPK signaling pathway. In conclusion, the present study indicated that SSRP1 regulated the proliferation and metastasis of glioma cells via the MAPK signaling pathway.
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spelling pubmed-57800192018-02-12 SSRP1 silencing inhibits the proliferation and malignancy of human glioma cells via the MAPK signaling pathway Liao, Jianming Tao, Xiang Ding, Qianshan Liu, Junhui Yang, Xue Yuan, Fan-En Yang, Ji-An Liu, Baohui Xiang, Guo-An Chen, Qianxue Oncol Rep Articles Structure-specific recognition protein 1 (SSRP1) has been considered as a potential biomarker, since aberrant high expression of SSRP1 has been detected in numerous malignant tumors. However, the correlation between the expression level of SSRP1 and glioma remains unclear. The present study attempted to investigate the role of SSRP1 in the pathogenesis of glioma. In the present study, our data revealed that SSRP1 overexpression was detected in glioma tissues at both the mRNA and protein levels using quantitative real-time RT-PCR and immunohistochemical analysis. We also demonstrated that the upregulated expression of SSRP1 was correlated with the World Health Organization (WHO) grade of glioma. The knockdown of SSRP1 by siRNA not only resulted in the inhibition of cell proliferation, but also significantly inhibited glioma cell migration and invasion. Mechanistic analyses revealed that SSRP1 depletion suppressed the activity of the phosphorylation of the MAPK signaling pathway. In conclusion, the present study indicated that SSRP1 regulated the proliferation and metastasis of glioma cells via the MAPK signaling pathway. D.A. Spandidos 2017-11 2017-09-21 /pmc/articles/PMC5780019/ /pubmed/29048646 http://dx.doi.org/10.3892/or.2017.5982 Text en Copyright: © Liao et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Liao, Jianming
Tao, Xiang
Ding, Qianshan
Liu, Junhui
Yang, Xue
Yuan, Fan-En
Yang, Ji-An
Liu, Baohui
Xiang, Guo-An
Chen, Qianxue
SSRP1 silencing inhibits the proliferation and malignancy of human glioma cells via the MAPK signaling pathway
title SSRP1 silencing inhibits the proliferation and malignancy of human glioma cells via the MAPK signaling pathway
title_full SSRP1 silencing inhibits the proliferation and malignancy of human glioma cells via the MAPK signaling pathway
title_fullStr SSRP1 silencing inhibits the proliferation and malignancy of human glioma cells via the MAPK signaling pathway
title_full_unstemmed SSRP1 silencing inhibits the proliferation and malignancy of human glioma cells via the MAPK signaling pathway
title_short SSRP1 silencing inhibits the proliferation and malignancy of human glioma cells via the MAPK signaling pathway
title_sort ssrp1 silencing inhibits the proliferation and malignancy of human glioma cells via the mapk signaling pathway
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5780019/
https://www.ncbi.nlm.nih.gov/pubmed/29048646
http://dx.doi.org/10.3892/or.2017.5982
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