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De novo synthesis of C4.4A in hepatocellular carcinoma promotes migration and invasion of tumor cells

C4.4A is a glycoprotein that is upregulated in several human malignancies, including colorectal, breast and renal cell carcinomas. Due to its highly restricted expression in healthy tissue, C4.4A was proposed as a potential diagnostic marker. Thus, the present study was designed to evaluate C4.4A ex...

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Autores principales: Görtz, Magdalena, Galli, Uwe, Longerich, Thomas, Zöller, Margot, Erb, Ulrike, Schemmer, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5780022/
https://www.ncbi.nlm.nih.gov/pubmed/29048672
http://dx.doi.org/10.3892/or.2017.5980
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author Görtz, Magdalena
Galli, Uwe
Longerich, Thomas
Zöller, Margot
Erb, Ulrike
Schemmer, Peter
author_facet Görtz, Magdalena
Galli, Uwe
Longerich, Thomas
Zöller, Margot
Erb, Ulrike
Schemmer, Peter
author_sort Görtz, Magdalena
collection PubMed
description C4.4A is a glycoprotein that is upregulated in several human malignancies, including colorectal, breast and renal cell carcinomas. Due to its highly restricted expression in healthy tissue, C4.4A was proposed as a potential diagnostic marker. Thus, the present study was designed to evaluate C4.4A expression and function in hepatocellular carcinoma (HCC) for the first time. Immunohistochemistry was performed to detect expression of C4.4A in human sections of healthy liver, primary HCC in the liver and metastatic HCC in the lung. To assess the contribution of C4.4A to HCC progression proliferation, apoptosis, migration and invasion assays were performed with C4.4A knockdown Huh7 and HepG2 cells. C4.4A is absent in healthy liver tissue. However, intense expression was seen in 59% of primary HCCs and strong expression in 80% of HCC lung metastases. C4.4A expression was also observed in human HCC cell lines, which strongly increased under hypoxic conditions. A C4.4A knock-down revealed that C4.4A is involved in both migration and invasion of HCC cells. Taken together, C4.4A expression in both primary and metastatic HCC suggests its potential value as a diagnostic marker for HCC. Due to its absence in healthy liver tissue, C4.4A might even serve as a possible therapeutic target, particularly for metastatic HCC.
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spelling pubmed-57800222018-02-12 De novo synthesis of C4.4A in hepatocellular carcinoma promotes migration and invasion of tumor cells Görtz, Magdalena Galli, Uwe Longerich, Thomas Zöller, Margot Erb, Ulrike Schemmer, Peter Oncol Rep Articles C4.4A is a glycoprotein that is upregulated in several human malignancies, including colorectal, breast and renal cell carcinomas. Due to its highly restricted expression in healthy tissue, C4.4A was proposed as a potential diagnostic marker. Thus, the present study was designed to evaluate C4.4A expression and function in hepatocellular carcinoma (HCC) for the first time. Immunohistochemistry was performed to detect expression of C4.4A in human sections of healthy liver, primary HCC in the liver and metastatic HCC in the lung. To assess the contribution of C4.4A to HCC progression proliferation, apoptosis, migration and invasion assays were performed with C4.4A knockdown Huh7 and HepG2 cells. C4.4A is absent in healthy liver tissue. However, intense expression was seen in 59% of primary HCCs and strong expression in 80% of HCC lung metastases. C4.4A expression was also observed in human HCC cell lines, which strongly increased under hypoxic conditions. A C4.4A knock-down revealed that C4.4A is involved in both migration and invasion of HCC cells. Taken together, C4.4A expression in both primary and metastatic HCC suggests its potential value as a diagnostic marker for HCC. Due to its absence in healthy liver tissue, C4.4A might even serve as a possible therapeutic target, particularly for metastatic HCC. D.A. Spandidos 2017-11 2017-09-20 /pmc/articles/PMC5780022/ /pubmed/29048672 http://dx.doi.org/10.3892/or.2017.5980 Text en Copyright: © Görtz et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Görtz, Magdalena
Galli, Uwe
Longerich, Thomas
Zöller, Margot
Erb, Ulrike
Schemmer, Peter
De novo synthesis of C4.4A in hepatocellular carcinoma promotes migration and invasion of tumor cells
title De novo synthesis of C4.4A in hepatocellular carcinoma promotes migration and invasion of tumor cells
title_full De novo synthesis of C4.4A in hepatocellular carcinoma promotes migration and invasion of tumor cells
title_fullStr De novo synthesis of C4.4A in hepatocellular carcinoma promotes migration and invasion of tumor cells
title_full_unstemmed De novo synthesis of C4.4A in hepatocellular carcinoma promotes migration and invasion of tumor cells
title_short De novo synthesis of C4.4A in hepatocellular carcinoma promotes migration and invasion of tumor cells
title_sort de novo synthesis of c4.4a in hepatocellular carcinoma promotes migration and invasion of tumor cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5780022/
https://www.ncbi.nlm.nih.gov/pubmed/29048672
http://dx.doi.org/10.3892/or.2017.5980
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