CDCA4, a downstream gene of the Nrf2 signaling pathway, regulates cell proliferation and apoptosis in the MCF-7/ADM human breast cancer cell line

The present study aimed to examine the effect of RNA interference targeting cell division cycle-associated protein 4 (CDCA4) on the proliferation and apoptosis of the MCF-7/ADR' human breast cancer cell line. CDCA4 has been shown to have a unique role in regulating cell cycle. In the present st...

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Detalles Bibliográficos
Autores principales: Xu, Yuju, Wu, Xianghua, Li, Fengxi, Huang, Daolai, Zhu, Wenxiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5780089/
https://www.ncbi.nlm.nih.gov/pubmed/29257222
http://dx.doi.org/10.3892/mmr.2017.8095
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author Xu, Yuju
Wu, Xianghua
Li, Fengxi
Huang, Daolai
Zhu, Wenxiang
author_facet Xu, Yuju
Wu, Xianghua
Li, Fengxi
Huang, Daolai
Zhu, Wenxiang
author_sort Xu, Yuju
collection PubMed
description The present study aimed to examine the effect of RNA interference targeting cell division cycle-associated protein 4 (CDCA4) on the proliferation and apoptosis of the MCF-7/ADR' human breast cancer cell line. CDCA4 has been shown to have a unique role in regulating cell cycle. In the present study, the expression of CDCA4 was suppressed by CDCA4-specific short hairpin (sh)RNA transfection of the human breast cancer cells, following which changes in the proliferation and apoptosis of the CDCA4-knockdown cells were compared with those of control shRNA-transfected cells. The results confirmed that CDCA4 RNA interference reduced the percentage of human breast cancer cells to <50%. In addition, RNA interference of CDCA4 resulted in a significant increase in the apoptotic rate of cells. Taken together, these results suggested that CDCA4 enhanced proliferation and reduced apoptosis in the MCF-7/ADM human breast cancer cells in vitro.
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spelling pubmed-57800892018-02-12 CDCA4, a downstream gene of the Nrf2 signaling pathway, regulates cell proliferation and apoptosis in the MCF-7/ADM human breast cancer cell line Xu, Yuju Wu, Xianghua Li, Fengxi Huang, Daolai Zhu, Wenxiang Mol Med Rep Articles The present study aimed to examine the effect of RNA interference targeting cell division cycle-associated protein 4 (CDCA4) on the proliferation and apoptosis of the MCF-7/ADR' human breast cancer cell line. CDCA4 has been shown to have a unique role in regulating cell cycle. In the present study, the expression of CDCA4 was suppressed by CDCA4-specific short hairpin (sh)RNA transfection of the human breast cancer cells, following which changes in the proliferation and apoptosis of the CDCA4-knockdown cells were compared with those of control shRNA-transfected cells. The results confirmed that CDCA4 RNA interference reduced the percentage of human breast cancer cells to <50%. In addition, RNA interference of CDCA4 resulted in a significant increase in the apoptotic rate of cells. Taken together, these results suggested that CDCA4 enhanced proliferation and reduced apoptosis in the MCF-7/ADM human breast cancer cells in vitro. D.A. Spandidos 2018-01 2017-11-15 /pmc/articles/PMC5780089/ /pubmed/29257222 http://dx.doi.org/10.3892/mmr.2017.8095 Text en Copyright: © Xu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Xu, Yuju
Wu, Xianghua
Li, Fengxi
Huang, Daolai
Zhu, Wenxiang
CDCA4, a downstream gene of the Nrf2 signaling pathway, regulates cell proliferation and apoptosis in the MCF-7/ADM human breast cancer cell line
title CDCA4, a downstream gene of the Nrf2 signaling pathway, regulates cell proliferation and apoptosis in the MCF-7/ADM human breast cancer cell line
title_full CDCA4, a downstream gene of the Nrf2 signaling pathway, regulates cell proliferation and apoptosis in the MCF-7/ADM human breast cancer cell line
title_fullStr CDCA4, a downstream gene of the Nrf2 signaling pathway, regulates cell proliferation and apoptosis in the MCF-7/ADM human breast cancer cell line
title_full_unstemmed CDCA4, a downstream gene of the Nrf2 signaling pathway, regulates cell proliferation and apoptosis in the MCF-7/ADM human breast cancer cell line
title_short CDCA4, a downstream gene of the Nrf2 signaling pathway, regulates cell proliferation and apoptosis in the MCF-7/ADM human breast cancer cell line
title_sort cdca4, a downstream gene of the nrf2 signaling pathway, regulates cell proliferation and apoptosis in the mcf-7/adm human breast cancer cell line
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5780089/
https://www.ncbi.nlm.nih.gov/pubmed/29257222
http://dx.doi.org/10.3892/mmr.2017.8095
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