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VEGF secreted by mesenchymal stem cells mediates the differentiation of endothelial progenitor cells into endothelial cells via paracrine mechanisms

Stem cell therapy is a promising treatment strategy for ischemic diseases. Mesenchymal stem cells (MSCs) and endothelial progenitor cells (EPCs) adhere to each other in the bone marrow cavity and in in vitro cultures. We have previously demonstrated that the adhesion between MSCs and EPCs is critica...

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Autores principales: Ge, Quanhu, Zhang, Hongwei, Hou, Jixue, Wan, Longfei, Cheng, Wenzhe, Wang, Xiaoyi, Dong, Dan, Chen, Congzhe, Xia, Jie, Guo, Jun, Chen, Xueling, Wu, Xiangwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5780109/
https://www.ncbi.nlm.nih.gov/pubmed/29138837
http://dx.doi.org/10.3892/mmr.2017.8059
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author Ge, Quanhu
Zhang, Hongwei
Hou, Jixue
Wan, Longfei
Cheng, Wenzhe
Wang, Xiaoyi
Dong, Dan
Chen, Congzhe
Xia, Jie
Guo, Jun
Chen, Xueling
Wu, Xiangwei
author_facet Ge, Quanhu
Zhang, Hongwei
Hou, Jixue
Wan, Longfei
Cheng, Wenzhe
Wang, Xiaoyi
Dong, Dan
Chen, Congzhe
Xia, Jie
Guo, Jun
Chen, Xueling
Wu, Xiangwei
author_sort Ge, Quanhu
collection PubMed
description Stem cell therapy is a promising treatment strategy for ischemic diseases. Mesenchymal stem cells (MSCs) and endothelial progenitor cells (EPCs) adhere to each other in the bone marrow cavity and in in vitro cultures. We have previously demonstrated that the adhesion between MSCs and EPCs is critical for MSC self-renewal and their multi-differentiation into osteoblasts and chondrocytes. In the present study, the influence of the indirect communication between EPCs and MSCs on the endothelial differentiation potential of EPCs was investigated, and the molecular mechanisms underlying MSC-mediated EPC differentiation were explored. The effects of vascular endothelial growth factor (VEGF), which is secreted by MSCs, on EPC differentiation via paracrine mechanisms were examined via co-culturing MSCs and EPCs. Reverse transcription-quantitative polymerase chain reaction and western blot analysis were used to detect the expression of genes and proteins of interest. The present results demonstrated that co-culturing EPCs with MSCs enhanced the expression of cluster of differentiation 31 and von Willebrand factor, which are specific markers of an endothelial phenotype, thus indicating that MSCs may influence the endothelial differentiation of EPCs in vitro. VEGF appeared to be critical to this process. These findings are important for the understanding of the biological interactions between MSCs and EPCs, and for the development of applications of stem cell-based therapy in the treatment of ischemic diseases.
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spelling pubmed-57801092018-02-12 VEGF secreted by mesenchymal stem cells mediates the differentiation of endothelial progenitor cells into endothelial cells via paracrine mechanisms Ge, Quanhu Zhang, Hongwei Hou, Jixue Wan, Longfei Cheng, Wenzhe Wang, Xiaoyi Dong, Dan Chen, Congzhe Xia, Jie Guo, Jun Chen, Xueling Wu, Xiangwei Mol Med Rep Articles Stem cell therapy is a promising treatment strategy for ischemic diseases. Mesenchymal stem cells (MSCs) and endothelial progenitor cells (EPCs) adhere to each other in the bone marrow cavity and in in vitro cultures. We have previously demonstrated that the adhesion between MSCs and EPCs is critical for MSC self-renewal and their multi-differentiation into osteoblasts and chondrocytes. In the present study, the influence of the indirect communication between EPCs and MSCs on the endothelial differentiation potential of EPCs was investigated, and the molecular mechanisms underlying MSC-mediated EPC differentiation were explored. The effects of vascular endothelial growth factor (VEGF), which is secreted by MSCs, on EPC differentiation via paracrine mechanisms were examined via co-culturing MSCs and EPCs. Reverse transcription-quantitative polymerase chain reaction and western blot analysis were used to detect the expression of genes and proteins of interest. The present results demonstrated that co-culturing EPCs with MSCs enhanced the expression of cluster of differentiation 31 and von Willebrand factor, which are specific markers of an endothelial phenotype, thus indicating that MSCs may influence the endothelial differentiation of EPCs in vitro. VEGF appeared to be critical to this process. These findings are important for the understanding of the biological interactions between MSCs and EPCs, and for the development of applications of stem cell-based therapy in the treatment of ischemic diseases. D.A. Spandidos 2018-01 2017-11-14 /pmc/articles/PMC5780109/ /pubmed/29138837 http://dx.doi.org/10.3892/mmr.2017.8059 Text en Copyright: © Ge et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Ge, Quanhu
Zhang, Hongwei
Hou, Jixue
Wan, Longfei
Cheng, Wenzhe
Wang, Xiaoyi
Dong, Dan
Chen, Congzhe
Xia, Jie
Guo, Jun
Chen, Xueling
Wu, Xiangwei
VEGF secreted by mesenchymal stem cells mediates the differentiation of endothelial progenitor cells into endothelial cells via paracrine mechanisms
title VEGF secreted by mesenchymal stem cells mediates the differentiation of endothelial progenitor cells into endothelial cells via paracrine mechanisms
title_full VEGF secreted by mesenchymal stem cells mediates the differentiation of endothelial progenitor cells into endothelial cells via paracrine mechanisms
title_fullStr VEGF secreted by mesenchymal stem cells mediates the differentiation of endothelial progenitor cells into endothelial cells via paracrine mechanisms
title_full_unstemmed VEGF secreted by mesenchymal stem cells mediates the differentiation of endothelial progenitor cells into endothelial cells via paracrine mechanisms
title_short VEGF secreted by mesenchymal stem cells mediates the differentiation of endothelial progenitor cells into endothelial cells via paracrine mechanisms
title_sort vegf secreted by mesenchymal stem cells mediates the differentiation of endothelial progenitor cells into endothelial cells via paracrine mechanisms
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5780109/
https://www.ncbi.nlm.nih.gov/pubmed/29138837
http://dx.doi.org/10.3892/mmr.2017.8059
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