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Two-component histidine kinase DRK1 is required for pathogenesis in Sporothrix schenckii

Sporothrix schenckii is a pathogenic dimorphic fungus with a global distribution. It grows in a multicellular hyphal form at 25°C and a unicellular yeast form at 37°C. The morphological switch from mold to yeast form is obligatory for establishing pathogenicity in S. schenckii. Two-component signali...

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Autores principales: Zhang, Zhenying, Hou, Binbin, Wu, Yong Zhuo, Wang, Ying, Liu, Xiaoming, Han, Shixin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5780148/
https://www.ncbi.nlm.nih.gov/pubmed/29115539
http://dx.doi.org/10.3892/mmr.2017.8005
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author Zhang, Zhenying
Hou, Binbin
Wu, Yong Zhuo
Wang, Ying
Liu, Xiaoming
Han, Shixin
author_facet Zhang, Zhenying
Hou, Binbin
Wu, Yong Zhuo
Wang, Ying
Liu, Xiaoming
Han, Shixin
author_sort Zhang, Zhenying
collection PubMed
description Sporothrix schenckii is a pathogenic dimorphic fungus with a global distribution. It grows in a multicellular hyphal form at 25°C and a unicellular yeast form at 37°C. The morphological switch from mold to yeast form is obligatory for establishing pathogenicity in S. schenckii. Two-component signaling systems are utilized by eukaryotes to sense and respond to external environmental changes. DRK1is a hybrid histidine kinase, which functions as a global regulator of dimorphism and virulence in Blastomyces dermatitidis and Histoplasma capsulatum. An intracellular soluble hybrid histidine kinase, homologous to DRK1 in B. dermatitidis, has previously been identified in S. schenckii and designated as SsDRK1. In the present study, the function of SsDRK1 was investigated using double stranded RNA interference mediated by Agrobacterium tumefaciens. SsDRK1 was demonstrated to be required for normal asexual development, yeast-phase cell formation, cell wall composition and integrity, melanin synthesis, transcription of the morphogenesis-associated gene Ste20 that is involved in the high osmolarity glycerol/mitogen-activated protein kinase pathway, and pathogenicity of S. schenckii in a murine model of cutaneous infection. Further investigations into the signals SsDRK1 responds to, and the interactions of upstream transmembrane hybrid histidine kinases with SsDRK1, are required to uncover novel targets for anti-fungal therapies.
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spelling pubmed-57801482018-02-05 Two-component histidine kinase DRK1 is required for pathogenesis in Sporothrix schenckii Zhang, Zhenying Hou, Binbin Wu, Yong Zhuo Wang, Ying Liu, Xiaoming Han, Shixin Mol Med Rep Articles Sporothrix schenckii is a pathogenic dimorphic fungus with a global distribution. It grows in a multicellular hyphal form at 25°C and a unicellular yeast form at 37°C. The morphological switch from mold to yeast form is obligatory for establishing pathogenicity in S. schenckii. Two-component signaling systems are utilized by eukaryotes to sense and respond to external environmental changes. DRK1is a hybrid histidine kinase, which functions as a global regulator of dimorphism and virulence in Blastomyces dermatitidis and Histoplasma capsulatum. An intracellular soluble hybrid histidine kinase, homologous to DRK1 in B. dermatitidis, has previously been identified in S. schenckii and designated as SsDRK1. In the present study, the function of SsDRK1 was investigated using double stranded RNA interference mediated by Agrobacterium tumefaciens. SsDRK1 was demonstrated to be required for normal asexual development, yeast-phase cell formation, cell wall composition and integrity, melanin synthesis, transcription of the morphogenesis-associated gene Ste20 that is involved in the high osmolarity glycerol/mitogen-activated protein kinase pathway, and pathogenicity of S. schenckii in a murine model of cutaneous infection. Further investigations into the signals SsDRK1 responds to, and the interactions of upstream transmembrane hybrid histidine kinases with SsDRK1, are required to uncover novel targets for anti-fungal therapies. D.A. Spandidos 2018-01 2017-11-07 /pmc/articles/PMC5780148/ /pubmed/29115539 http://dx.doi.org/10.3892/mmr.2017.8005 Text en Copyright: © Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Zhang, Zhenying
Hou, Binbin
Wu, Yong Zhuo
Wang, Ying
Liu, Xiaoming
Han, Shixin
Two-component histidine kinase DRK1 is required for pathogenesis in Sporothrix schenckii
title Two-component histidine kinase DRK1 is required for pathogenesis in Sporothrix schenckii
title_full Two-component histidine kinase DRK1 is required for pathogenesis in Sporothrix schenckii
title_fullStr Two-component histidine kinase DRK1 is required for pathogenesis in Sporothrix schenckii
title_full_unstemmed Two-component histidine kinase DRK1 is required for pathogenesis in Sporothrix schenckii
title_short Two-component histidine kinase DRK1 is required for pathogenesis in Sporothrix schenckii
title_sort two-component histidine kinase drk1 is required for pathogenesis in sporothrix schenckii
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5780148/
https://www.ncbi.nlm.nih.gov/pubmed/29115539
http://dx.doi.org/10.3892/mmr.2017.8005
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