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MicroRNA-103 regulates tumorigenesis in colorectal cancer by targeting ZO-1

Given the emerging role of microRNAs (miRs) in cancer progression, the present study investigated the role and underlying mechanism of miR-103 in colorectal cancer (CRC). Reverse transcription-quantitative polymerase chain reaction was conducted to quantify the expression levels of miR-103 in clinic...

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Detalles Bibliográficos
Autores principales: Ke, Jin, Shao, Weiwei, Jiang, Yasu, Xu, Junfei, Li, Feng, Qin, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5780155/
https://www.ncbi.nlm.nih.gov/pubmed/29115525
http://dx.doi.org/10.3892/mmr.2017.8007
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author Ke, Jin
Shao, Weiwei
Jiang, Yasu
Xu, Junfei
Li, Feng
Qin, Jun
author_facet Ke, Jin
Shao, Weiwei
Jiang, Yasu
Xu, Junfei
Li, Feng
Qin, Jun
author_sort Ke, Jin
collection PubMed
description Given the emerging role of microRNAs (miRs) in cancer progression, the present study investigated the role and underlying mechanism of miR-103 in colorectal cancer (CRC). Reverse transcription-quantitative polymerase chain reaction was conducted to quantify the expression levels of miR-103 in clinical specimens and cell lines. The role of miR-103 in CRC was examined using MTT, colony formation and transwell assays. In addition, a luciferase reporter assay was used to confirm an associated between the 3′ untranslated region of zonula occuldens-1 (ZO-1) and miR-103. The results demonstrated that miR-103 was upregulated in CRC. Overexpression of miR-103 promoted CRC cell proliferation and migration in vitro, whereas downregulation of miR-103 inhibited cell proliferation and migration. ZO-1 was identified as a direct target of miR-103, revealing its expression to be inversely correlated with miR-103 expression in CRC samples. In conclusion, the present study revealed that miR-103 has strong tumor-promoting effects via of targeting ZO-1 in CRC and has potential development of miRNA-based targeted approaches for the treatment of CRC.
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spelling pubmed-57801552018-02-05 MicroRNA-103 regulates tumorigenesis in colorectal cancer by targeting ZO-1 Ke, Jin Shao, Weiwei Jiang, Yasu Xu, Junfei Li, Feng Qin, Jun Mol Med Rep Articles Given the emerging role of microRNAs (miRs) in cancer progression, the present study investigated the role and underlying mechanism of miR-103 in colorectal cancer (CRC). Reverse transcription-quantitative polymerase chain reaction was conducted to quantify the expression levels of miR-103 in clinical specimens and cell lines. The role of miR-103 in CRC was examined using MTT, colony formation and transwell assays. In addition, a luciferase reporter assay was used to confirm an associated between the 3′ untranslated region of zonula occuldens-1 (ZO-1) and miR-103. The results demonstrated that miR-103 was upregulated in CRC. Overexpression of miR-103 promoted CRC cell proliferation and migration in vitro, whereas downregulation of miR-103 inhibited cell proliferation and migration. ZO-1 was identified as a direct target of miR-103, revealing its expression to be inversely correlated with miR-103 expression in CRC samples. In conclusion, the present study revealed that miR-103 has strong tumor-promoting effects via of targeting ZO-1 in CRC and has potential development of miRNA-based targeted approaches for the treatment of CRC. D.A. Spandidos 2018-01 2017-11-07 /pmc/articles/PMC5780155/ /pubmed/29115525 http://dx.doi.org/10.3892/mmr.2017.8007 Text en Copyright: © Ke et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Ke, Jin
Shao, Weiwei
Jiang, Yasu
Xu, Junfei
Li, Feng
Qin, Jun
MicroRNA-103 regulates tumorigenesis in colorectal cancer by targeting ZO-1
title MicroRNA-103 regulates tumorigenesis in colorectal cancer by targeting ZO-1
title_full MicroRNA-103 regulates tumorigenesis in colorectal cancer by targeting ZO-1
title_fullStr MicroRNA-103 regulates tumorigenesis in colorectal cancer by targeting ZO-1
title_full_unstemmed MicroRNA-103 regulates tumorigenesis in colorectal cancer by targeting ZO-1
title_short MicroRNA-103 regulates tumorigenesis in colorectal cancer by targeting ZO-1
title_sort microrna-103 regulates tumorigenesis in colorectal cancer by targeting zo-1
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5780155/
https://www.ncbi.nlm.nih.gov/pubmed/29115525
http://dx.doi.org/10.3892/mmr.2017.8007
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