Cargando…
Caprine Monocytes Release Extracellular Traps against Neospora caninum In Vitro
Neospora caninum is an obligate intracellular apicomplexan parasite that causes reproductive loss and severe economic losses in dairy and goat industry. In the present study, we aim to investigate the effects of N. caninum tachyzoites on the release of extracellular traps (ETs) in caprine monocytes...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5780343/ https://www.ncbi.nlm.nih.gov/pubmed/29403487 http://dx.doi.org/10.3389/fimmu.2017.02016 |
_version_ | 1783294716393029632 |
---|---|
author | Yang, Zhengtao Wei, Zhengkai Hermosilla, Carlos Taubert, Anja He, Xuexiu Wang, Xiaocen Gong, Pengtao Li, Jianhua Zhang, Xichen |
author_facet | Yang, Zhengtao Wei, Zhengkai Hermosilla, Carlos Taubert, Anja He, Xuexiu Wang, Xiaocen Gong, Pengtao Li, Jianhua Zhang, Xichen |
author_sort | Yang, Zhengtao |
collection | PubMed |
description | Neospora caninum is an obligate intracellular apicomplexan parasite that causes reproductive loss and severe economic losses in dairy and goat industry. In the present study, we aim to investigate the effects of N. caninum tachyzoites on the release of extracellular traps (ETs) in caprine monocytes and furthermore elucidated parts of its molecular mechanisms. N. caninum tachyzoite-induced monocytes-derived ETs formation was detected by scanning electron microscopy. H3 and myeloperoxidase (MPO) within monocyte-ETs structures were examined using laser scanning confocal microscopy analyses. The results showed that N. caninum tachyzoites were not only able to trigger ETs formation in caprine monocytes, but also that monocyte-released ETs were capable of entrapping viable tachyzoites. Histones and MPO were found to be decorating the DNA within the monocytes derived-ETs structures thus proving the classical components of ETs. Furthermore, inhibitors of NADPH oxidase-, MPO-, ERK 1/2-, or p38 MAPK-signaling pathway significantly decreased N. caninum tachyzoite-triggered caprine monocyte-derived ETosis. This is the first report of ETs release extruded from caprine monocytes after N. caninum exposure and thus showing that this early innate immune effector mechanism might be relevant during the acute phase of caprine neosporosis. |
format | Online Article Text |
id | pubmed-5780343 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-57803432018-02-05 Caprine Monocytes Release Extracellular Traps against Neospora caninum In Vitro Yang, Zhengtao Wei, Zhengkai Hermosilla, Carlos Taubert, Anja He, Xuexiu Wang, Xiaocen Gong, Pengtao Li, Jianhua Zhang, Xichen Front Immunol Immunology Neospora caninum is an obligate intracellular apicomplexan parasite that causes reproductive loss and severe economic losses in dairy and goat industry. In the present study, we aim to investigate the effects of N. caninum tachyzoites on the release of extracellular traps (ETs) in caprine monocytes and furthermore elucidated parts of its molecular mechanisms. N. caninum tachyzoite-induced monocytes-derived ETs formation was detected by scanning electron microscopy. H3 and myeloperoxidase (MPO) within monocyte-ETs structures were examined using laser scanning confocal microscopy analyses. The results showed that N. caninum tachyzoites were not only able to trigger ETs formation in caprine monocytes, but also that monocyte-released ETs were capable of entrapping viable tachyzoites. Histones and MPO were found to be decorating the DNA within the monocytes derived-ETs structures thus proving the classical components of ETs. Furthermore, inhibitors of NADPH oxidase-, MPO-, ERK 1/2-, or p38 MAPK-signaling pathway significantly decreased N. caninum tachyzoite-triggered caprine monocyte-derived ETosis. This is the first report of ETs release extruded from caprine monocytes after N. caninum exposure and thus showing that this early innate immune effector mechanism might be relevant during the acute phase of caprine neosporosis. Frontiers Media S.A. 2018-01-19 /pmc/articles/PMC5780343/ /pubmed/29403487 http://dx.doi.org/10.3389/fimmu.2017.02016 Text en Copyright © 2018 Yang, Wei, Hermosilla, Taubert, He, Wang, Gong, Li and Zhang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Yang, Zhengtao Wei, Zhengkai Hermosilla, Carlos Taubert, Anja He, Xuexiu Wang, Xiaocen Gong, Pengtao Li, Jianhua Zhang, Xichen Caprine Monocytes Release Extracellular Traps against Neospora caninum In Vitro |
title | Caprine Monocytes Release Extracellular Traps against Neospora caninum In Vitro |
title_full | Caprine Monocytes Release Extracellular Traps against Neospora caninum In Vitro |
title_fullStr | Caprine Monocytes Release Extracellular Traps against Neospora caninum In Vitro |
title_full_unstemmed | Caprine Monocytes Release Extracellular Traps against Neospora caninum In Vitro |
title_short | Caprine Monocytes Release Extracellular Traps against Neospora caninum In Vitro |
title_sort | caprine monocytes release extracellular traps against neospora caninum in vitro |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5780343/ https://www.ncbi.nlm.nih.gov/pubmed/29403487 http://dx.doi.org/10.3389/fimmu.2017.02016 |
work_keys_str_mv | AT yangzhengtao caprinemonocytesreleaseextracellulartrapsagainstneosporacaninuminvitro AT weizhengkai caprinemonocytesreleaseextracellulartrapsagainstneosporacaninuminvitro AT hermosillacarlos caprinemonocytesreleaseextracellulartrapsagainstneosporacaninuminvitro AT taubertanja caprinemonocytesreleaseextracellulartrapsagainstneosporacaninuminvitro AT hexuexiu caprinemonocytesreleaseextracellulartrapsagainstneosporacaninuminvitro AT wangxiaocen caprinemonocytesreleaseextracellulartrapsagainstneosporacaninuminvitro AT gongpengtao caprinemonocytesreleaseextracellulartrapsagainstneosporacaninuminvitro AT lijianhua caprinemonocytesreleaseextracellulartrapsagainstneosporacaninuminvitro AT zhangxichen caprinemonocytesreleaseextracellulartrapsagainstneosporacaninuminvitro |