An Improved Total RNA Extraction Method for White Jelly Mushroom Tremella fuciformis Rich in Polysaccharides

An improved method for extracting high quality and quantity RNA from a jelly mushroom and a dimorphic fungus—Tremella fuciformis which is especially rich in polysaccharides, is described. RNA was extracted from T. fuciformis mycelium M1332 and its parental monokaryotic yeast-like cells Y13 and Y32....

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Autores principales: Zhu, Hanyu, Sun, Xueyan, Liu, Dongmei, Zheng, Liesheng, Chen, Liguo, Ma, Aimin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Mycology 2017
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5780378/
https://www.ncbi.nlm.nih.gov/pubmed/29371814
http://dx.doi.org/10.5941/MYCO.2017.45.4.434
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author Zhu, Hanyu
Sun, Xueyan
Liu, Dongmei
Zheng, Liesheng
Chen, Liguo
Ma, Aimin
author_facet Zhu, Hanyu
Sun, Xueyan
Liu, Dongmei
Zheng, Liesheng
Chen, Liguo
Ma, Aimin
author_sort Zhu, Hanyu
collection PubMed
description An improved method for extracting high quality and quantity RNA from a jelly mushroom and a dimorphic fungus—Tremella fuciformis which is especially rich in polysaccharides, is described. RNA was extracted from T. fuciformis mycelium M1332 and its parental monokaryotic yeast-like cells Y13 and Y32. The A260/280 and A260/230 ratios were both approximately 2, and the RNA integrity number was larger than 8.9. The yields of RNA were between 108 and 213 µg/g fresh wt. Downstream molecular applications including reverse transcriptional PCR and quantitative real-time PCR were also performed. This protocol is reliable and may be widely applicable for total RNA extraction from other jelly mushrooms or filamentous fungi rich in polysaccharides.
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spelling pubmed-57803782018-01-25 An Improved Total RNA Extraction Method for White Jelly Mushroom Tremella fuciformis Rich in Polysaccharides Zhu, Hanyu Sun, Xueyan Liu, Dongmei Zheng, Liesheng Chen, Liguo Ma, Aimin Mycobiology Research Note An improved method for extracting high quality and quantity RNA from a jelly mushroom and a dimorphic fungus—Tremella fuciformis which is especially rich in polysaccharides, is described. RNA was extracted from T. fuciformis mycelium M1332 and its parental monokaryotic yeast-like cells Y13 and Y32. The A260/280 and A260/230 ratios were both approximately 2, and the RNA integrity number was larger than 8.9. The yields of RNA were between 108 and 213 µg/g fresh wt. Downstream molecular applications including reverse transcriptional PCR and quantitative real-time PCR were also performed. This protocol is reliable and may be widely applicable for total RNA extraction from other jelly mushrooms or filamentous fungi rich in polysaccharides. The Korean Society of Mycology 2017-12 2017-12-31 /pmc/articles/PMC5780378/ /pubmed/29371814 http://dx.doi.org/10.5941/MYCO.2017.45.4.434 Text en © The Korean Society of Mycology http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Note
Zhu, Hanyu
Sun, Xueyan
Liu, Dongmei
Zheng, Liesheng
Chen, Liguo
Ma, Aimin
An Improved Total RNA Extraction Method for White Jelly Mushroom Tremella fuciformis Rich in Polysaccharides
title An Improved Total RNA Extraction Method for White Jelly Mushroom Tremella fuciformis Rich in Polysaccharides
title_full An Improved Total RNA Extraction Method for White Jelly Mushroom Tremella fuciformis Rich in Polysaccharides
title_fullStr An Improved Total RNA Extraction Method for White Jelly Mushroom Tremella fuciformis Rich in Polysaccharides
title_full_unstemmed An Improved Total RNA Extraction Method for White Jelly Mushroom Tremella fuciformis Rich in Polysaccharides
title_short An Improved Total RNA Extraction Method for White Jelly Mushroom Tremella fuciformis Rich in Polysaccharides
title_sort improved total rna extraction method for white jelly mushroom tremella fuciformis rich in polysaccharides
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5780378/
https://www.ncbi.nlm.nih.gov/pubmed/29371814
http://dx.doi.org/10.5941/MYCO.2017.45.4.434
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