CREB-binding protein plays key roles in juvenile hormone action in the red flour beetle, Tribolium Castaneum

Juvenile hormones (JH) and ecdysteroids regulate many biological and metabolic processes. CREB-binding protein (CBP) is a transcriptional co-regulator with histone acetyltransferase (HAT) activity. Therefore, CBP is involved in activation of many transcription factors that regulate expression of gen...

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Autores principales: Xu, Jingjing, Roy, Amit, Palli, Subba Reddy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5780420/
https://www.ncbi.nlm.nih.gov/pubmed/29362416
http://dx.doi.org/10.1038/s41598-018-19667-6
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author Xu, Jingjing
Roy, Amit
Palli, Subba Reddy
author_facet Xu, Jingjing
Roy, Amit
Palli, Subba Reddy
author_sort Xu, Jingjing
collection PubMed
description Juvenile hormones (JH) and ecdysteroids regulate many biological and metabolic processes. CREB-binding protein (CBP) is a transcriptional co-regulator with histone acetyltransferase (HAT) activity. Therefore, CBP is involved in activation of many transcription factors that regulate expression of genes associated with postembryonic development in insects. However, the function of CBP in JH action in insects is not well understood. Hence, we studied the role of CBP in JH action in the red flour beetle, Tribolium castaneum and the Tribolium cell line. CBP knockdown caused a decrease in JH induction of genes, Kr-h1, 4EBP and G13402 in T. castaneum larvae, adults and TcA cells whereas, Trichostatin A [TSA, a histone deacetylase (HDAC) inhibitor] induced the expression of these JH-response genes. Western blot analysis with specific antibodies revealed the requirement of CBP for the acetylation of H3K18 and H3K27 in both T. castaneum and TcA cells. Chromatin immunoprecipitation (Chip) assays showed the importance of CBP-mediated acetylation of H3K27 for JH induction of Kr-h1, 4EBP, and G13402 in TcA cells. These data suggest that CBP plays an important role in JH action in the model insect, T.castaneum.
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spelling pubmed-57804202018-02-06 CREB-binding protein plays key roles in juvenile hormone action in the red flour beetle, Tribolium Castaneum Xu, Jingjing Roy, Amit Palli, Subba Reddy Sci Rep Article Juvenile hormones (JH) and ecdysteroids regulate many biological and metabolic processes. CREB-binding protein (CBP) is a transcriptional co-regulator with histone acetyltransferase (HAT) activity. Therefore, CBP is involved in activation of many transcription factors that regulate expression of genes associated with postembryonic development in insects. However, the function of CBP in JH action in insects is not well understood. Hence, we studied the role of CBP in JH action in the red flour beetle, Tribolium castaneum and the Tribolium cell line. CBP knockdown caused a decrease in JH induction of genes, Kr-h1, 4EBP and G13402 in T. castaneum larvae, adults and TcA cells whereas, Trichostatin A [TSA, a histone deacetylase (HDAC) inhibitor] induced the expression of these JH-response genes. Western blot analysis with specific antibodies revealed the requirement of CBP for the acetylation of H3K18 and H3K27 in both T. castaneum and TcA cells. Chromatin immunoprecipitation (Chip) assays showed the importance of CBP-mediated acetylation of H3K27 for JH induction of Kr-h1, 4EBP, and G13402 in TcA cells. These data suggest that CBP plays an important role in JH action in the model insect, T.castaneum. Nature Publishing Group UK 2018-01-23 /pmc/articles/PMC5780420/ /pubmed/29362416 http://dx.doi.org/10.1038/s41598-018-19667-6 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Xu, Jingjing
Roy, Amit
Palli, Subba Reddy
CREB-binding protein plays key roles in juvenile hormone action in the red flour beetle, Tribolium Castaneum
title CREB-binding protein plays key roles in juvenile hormone action in the red flour beetle, Tribolium Castaneum
title_full CREB-binding protein plays key roles in juvenile hormone action in the red flour beetle, Tribolium Castaneum
title_fullStr CREB-binding protein plays key roles in juvenile hormone action in the red flour beetle, Tribolium Castaneum
title_full_unstemmed CREB-binding protein plays key roles in juvenile hormone action in the red flour beetle, Tribolium Castaneum
title_short CREB-binding protein plays key roles in juvenile hormone action in the red flour beetle, Tribolium Castaneum
title_sort creb-binding protein plays key roles in juvenile hormone action in the red flour beetle, tribolium castaneum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5780420/
https://www.ncbi.nlm.nih.gov/pubmed/29362416
http://dx.doi.org/10.1038/s41598-018-19667-6
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