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Comparison between direct contact and extract exposure methods for PFO cytotoxicity evaluation
A series of recent acute blindness cases following non–complicated retinal detachment surgery caused the release of several health alerts in Spain. The blindness was attributed to certain lots of perfluoro-octane (PFO; a volatile and transient medical device). Similar cases have been reported in oth...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5780508/ https://www.ncbi.nlm.nih.gov/pubmed/29362382 http://dx.doi.org/10.1038/s41598-018-19428-5 |
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author | Srivastava, Girish K. Alonso-Alonso, Maria L. Fernandez-Bueno, Ivan Garcia-Gutierrez, Maria T. Rull, Fernando Medina, Jesús Coco, Rosa M. Pastor, J. Carlos |
author_facet | Srivastava, Girish K. Alonso-Alonso, Maria L. Fernandez-Bueno, Ivan Garcia-Gutierrez, Maria T. Rull, Fernando Medina, Jesús Coco, Rosa M. Pastor, J. Carlos |
author_sort | Srivastava, Girish K. |
collection | PubMed |
description | A series of recent acute blindness cases following non–complicated retinal detachment surgery caused the release of several health alerts in Spain. The blindness was attributed to certain lots of perfluoro-octane (PFO; a volatile and transient medical device). Similar cases have been reported in other countries. This has raised questions regarding the validity of cytotoxicity test methods currently used to certify the safety of PFO lots. The tests were performed according to the International Organization for Standardization (ISO) norms, using the extract dilution method or the indirect contact method as applied to L929 cells, a line derived from mouse fibroblasts. The limitations of those methods have been resolved in this study by proposing a new cytotoxicity test method for volatile substances. The new method requires direct contact of the tested substance with cells that are similar to those exposed to the substance in the clinical setting. This approach includes a few new technical steps that are crucial for detecting cytotoxicity. Our new method detected toxic PFO lots that corresponded to the lots producing clinical blindness, which previous methods failed to detect. The study suggests applying this new method to avoid occurrence of such cases of blindness. |
format | Online Article Text |
id | pubmed-5780508 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-57805082018-02-06 Comparison between direct contact and extract exposure methods for PFO cytotoxicity evaluation Srivastava, Girish K. Alonso-Alonso, Maria L. Fernandez-Bueno, Ivan Garcia-Gutierrez, Maria T. Rull, Fernando Medina, Jesús Coco, Rosa M. Pastor, J. Carlos Sci Rep Article A series of recent acute blindness cases following non–complicated retinal detachment surgery caused the release of several health alerts in Spain. The blindness was attributed to certain lots of perfluoro-octane (PFO; a volatile and transient medical device). Similar cases have been reported in other countries. This has raised questions regarding the validity of cytotoxicity test methods currently used to certify the safety of PFO lots. The tests were performed according to the International Organization for Standardization (ISO) norms, using the extract dilution method or the indirect contact method as applied to L929 cells, a line derived from mouse fibroblasts. The limitations of those methods have been resolved in this study by proposing a new cytotoxicity test method for volatile substances. The new method requires direct contact of the tested substance with cells that are similar to those exposed to the substance in the clinical setting. This approach includes a few new technical steps that are crucial for detecting cytotoxicity. Our new method detected toxic PFO lots that corresponded to the lots producing clinical blindness, which previous methods failed to detect. The study suggests applying this new method to avoid occurrence of such cases of blindness. Nature Publishing Group UK 2018-01-23 /pmc/articles/PMC5780508/ /pubmed/29362382 http://dx.doi.org/10.1038/s41598-018-19428-5 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Srivastava, Girish K. Alonso-Alonso, Maria L. Fernandez-Bueno, Ivan Garcia-Gutierrez, Maria T. Rull, Fernando Medina, Jesús Coco, Rosa M. Pastor, J. Carlos Comparison between direct contact and extract exposure methods for PFO cytotoxicity evaluation |
title | Comparison between direct contact and extract exposure methods for PFO cytotoxicity evaluation |
title_full | Comparison between direct contact and extract exposure methods for PFO cytotoxicity evaluation |
title_fullStr | Comparison between direct contact and extract exposure methods for PFO cytotoxicity evaluation |
title_full_unstemmed | Comparison between direct contact and extract exposure methods for PFO cytotoxicity evaluation |
title_short | Comparison between direct contact and extract exposure methods for PFO cytotoxicity evaluation |
title_sort | comparison between direct contact and extract exposure methods for pfo cytotoxicity evaluation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5780508/ https://www.ncbi.nlm.nih.gov/pubmed/29362382 http://dx.doi.org/10.1038/s41598-018-19428-5 |
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