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Mycobacterium tuberculosis Acquires Limited Genetic Diversity in Prolonged Infections, Reactivations and Transmissions Involving Multiple Hosts
Background: Mycobacterium tuberculosis (MTB) has limited ability to acquire variability. Analysis of its microevolution might help us to evaluate the pathways followed to acquire greater infective success. Whole-genome sequencing (WGS) in the analysis of the transmission of MTB has elucidated the ma...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5780704/ https://www.ncbi.nlm.nih.gov/pubmed/29403447 http://dx.doi.org/10.3389/fmicb.2017.02661 |
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author | Herranz, Marta Pole, Ilva Ozere, Iveta Chiner-Oms, Álvaro Martínez-Lirola, Miguel Pérez-García, Felipe Gijón, Paloma Serrano, María Jesús Ruiz Romero, Laura Clotet Cuevas, Oscar Comas, Iñaki Bouza, Emilio Pérez-Lago, Laura García-de-Viedma, Darío |
author_facet | Herranz, Marta Pole, Ilva Ozere, Iveta Chiner-Oms, Álvaro Martínez-Lirola, Miguel Pérez-García, Felipe Gijón, Paloma Serrano, María Jesús Ruiz Romero, Laura Clotet Cuevas, Oscar Comas, Iñaki Bouza, Emilio Pérez-Lago, Laura García-de-Viedma, Darío |
author_sort | Herranz, Marta |
collection | PubMed |
description | Background: Mycobacterium tuberculosis (MTB) has limited ability to acquire variability. Analysis of its microevolution might help us to evaluate the pathways followed to acquire greater infective success. Whole-genome sequencing (WGS) in the analysis of the transmission of MTB has elucidated the magnitude of variability in MTB. Analysis of transmission currently depends on the identification of clusters, according to the threshold of variability (<5 SNPs) between isolates. Objective: We evaluated whether the acquisition of variability in MTB, was more frequent in situations which could favor it, namely intrapatient, prolonged infections or reactivations and interpatient transmissions involving multiple sequential hosts. Methods: We used WGS to analyze the accumulation of variability in sequential isolates from prolonged infections or translations from latency to reactivation. We then measured microevolution in transmission clusters with prolonged transmission time, high number of involved cases, simultaneous involvement of latency and active transmission. Results: Intrapatient and interpatient acquisition of variability was limited, within the ranges expected according to the thresholds of variability proposed, even though bursts of variability were observed. Conclusions: The thresholds of variability proposed for MTB seem to be valid in most circumstances, including those theoretically favoring acquisition of variability. Our data point to multifactorial modulation of microevolution, although further studies are necessary to elucidate the factors underlying this modulation. |
format | Online Article Text |
id | pubmed-5780704 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-57807042018-02-05 Mycobacterium tuberculosis Acquires Limited Genetic Diversity in Prolonged Infections, Reactivations and Transmissions Involving Multiple Hosts Herranz, Marta Pole, Ilva Ozere, Iveta Chiner-Oms, Álvaro Martínez-Lirola, Miguel Pérez-García, Felipe Gijón, Paloma Serrano, María Jesús Ruiz Romero, Laura Clotet Cuevas, Oscar Comas, Iñaki Bouza, Emilio Pérez-Lago, Laura García-de-Viedma, Darío Front Microbiol Microbiology Background: Mycobacterium tuberculosis (MTB) has limited ability to acquire variability. Analysis of its microevolution might help us to evaluate the pathways followed to acquire greater infective success. Whole-genome sequencing (WGS) in the analysis of the transmission of MTB has elucidated the magnitude of variability in MTB. Analysis of transmission currently depends on the identification of clusters, according to the threshold of variability (<5 SNPs) between isolates. Objective: We evaluated whether the acquisition of variability in MTB, was more frequent in situations which could favor it, namely intrapatient, prolonged infections or reactivations and interpatient transmissions involving multiple sequential hosts. Methods: We used WGS to analyze the accumulation of variability in sequential isolates from prolonged infections or translations from latency to reactivation. We then measured microevolution in transmission clusters with prolonged transmission time, high number of involved cases, simultaneous involvement of latency and active transmission. Results: Intrapatient and interpatient acquisition of variability was limited, within the ranges expected according to the thresholds of variability proposed, even though bursts of variability were observed. Conclusions: The thresholds of variability proposed for MTB seem to be valid in most circumstances, including those theoretically favoring acquisition of variability. Our data point to multifactorial modulation of microevolution, although further studies are necessary to elucidate the factors underlying this modulation. Frontiers Media S.A. 2018-01-19 /pmc/articles/PMC5780704/ /pubmed/29403447 http://dx.doi.org/10.3389/fmicb.2017.02661 Text en Copyright © 2018 Herranz, Pole, Ozere, Chiner-Oms, Martínez-Lirola, Pérez-García, Gijón, Serrano, Romero, Cuevas, Comas, Bouza, Pérez-Lago and García-de-Viedma. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Herranz, Marta Pole, Ilva Ozere, Iveta Chiner-Oms, Álvaro Martínez-Lirola, Miguel Pérez-García, Felipe Gijón, Paloma Serrano, María Jesús Ruiz Romero, Laura Clotet Cuevas, Oscar Comas, Iñaki Bouza, Emilio Pérez-Lago, Laura García-de-Viedma, Darío Mycobacterium tuberculosis Acquires Limited Genetic Diversity in Prolonged Infections, Reactivations and Transmissions Involving Multiple Hosts |
title | Mycobacterium tuberculosis Acquires Limited Genetic Diversity in Prolonged Infections, Reactivations and Transmissions Involving Multiple Hosts |
title_full | Mycobacterium tuberculosis Acquires Limited Genetic Diversity in Prolonged Infections, Reactivations and Transmissions Involving Multiple Hosts |
title_fullStr | Mycobacterium tuberculosis Acquires Limited Genetic Diversity in Prolonged Infections, Reactivations and Transmissions Involving Multiple Hosts |
title_full_unstemmed | Mycobacterium tuberculosis Acquires Limited Genetic Diversity in Prolonged Infections, Reactivations and Transmissions Involving Multiple Hosts |
title_short | Mycobacterium tuberculosis Acquires Limited Genetic Diversity in Prolonged Infections, Reactivations and Transmissions Involving Multiple Hosts |
title_sort | mycobacterium tuberculosis acquires limited genetic diversity in prolonged infections, reactivations and transmissions involving multiple hosts |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5780704/ https://www.ncbi.nlm.nih.gov/pubmed/29403447 http://dx.doi.org/10.3389/fmicb.2017.02661 |
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