Cargando…

Mycobacterium tuberculosis Acquires Limited Genetic Diversity in Prolonged Infections, Reactivations and Transmissions Involving Multiple Hosts

Background: Mycobacterium tuberculosis (MTB) has limited ability to acquire variability. Analysis of its microevolution might help us to evaluate the pathways followed to acquire greater infective success. Whole-genome sequencing (WGS) in the analysis of the transmission of MTB has elucidated the ma...

Descripción completa

Detalles Bibliográficos
Autores principales: Herranz, Marta, Pole, Ilva, Ozere, Iveta, Chiner-Oms, Álvaro, Martínez-Lirola, Miguel, Pérez-García, Felipe, Gijón, Paloma, Serrano, María Jesús Ruiz, Romero, Laura Clotet, Cuevas, Oscar, Comas, Iñaki, Bouza, Emilio, Pérez-Lago, Laura, García-de-Viedma, Darío
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5780704/
https://www.ncbi.nlm.nih.gov/pubmed/29403447
http://dx.doi.org/10.3389/fmicb.2017.02661
_version_ 1783294790313443328
author Herranz, Marta
Pole, Ilva
Ozere, Iveta
Chiner-Oms, Álvaro
Martínez-Lirola, Miguel
Pérez-García, Felipe
Gijón, Paloma
Serrano, María Jesús Ruiz
Romero, Laura Clotet
Cuevas, Oscar
Comas, Iñaki
Bouza, Emilio
Pérez-Lago, Laura
García-de-Viedma, Darío
author_facet Herranz, Marta
Pole, Ilva
Ozere, Iveta
Chiner-Oms, Álvaro
Martínez-Lirola, Miguel
Pérez-García, Felipe
Gijón, Paloma
Serrano, María Jesús Ruiz
Romero, Laura Clotet
Cuevas, Oscar
Comas, Iñaki
Bouza, Emilio
Pérez-Lago, Laura
García-de-Viedma, Darío
author_sort Herranz, Marta
collection PubMed
description Background: Mycobacterium tuberculosis (MTB) has limited ability to acquire variability. Analysis of its microevolution might help us to evaluate the pathways followed to acquire greater infective success. Whole-genome sequencing (WGS) in the analysis of the transmission of MTB has elucidated the magnitude of variability in MTB. Analysis of transmission currently depends on the identification of clusters, according to the threshold of variability (<5 SNPs) between isolates. Objective: We evaluated whether the acquisition of variability in MTB, was more frequent in situations which could favor it, namely intrapatient, prolonged infections or reactivations and interpatient transmissions involving multiple sequential hosts. Methods: We used WGS to analyze the accumulation of variability in sequential isolates from prolonged infections or translations from latency to reactivation. We then measured microevolution in transmission clusters with prolonged transmission time, high number of involved cases, simultaneous involvement of latency and active transmission. Results: Intrapatient and interpatient acquisition of variability was limited, within the ranges expected according to the thresholds of variability proposed, even though bursts of variability were observed. Conclusions: The thresholds of variability proposed for MTB seem to be valid in most circumstances, including those theoretically favoring acquisition of variability. Our data point to multifactorial modulation of microevolution, although further studies are necessary to elucidate the factors underlying this modulation.
format Online
Article
Text
id pubmed-5780704
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-57807042018-02-05 Mycobacterium tuberculosis Acquires Limited Genetic Diversity in Prolonged Infections, Reactivations and Transmissions Involving Multiple Hosts Herranz, Marta Pole, Ilva Ozere, Iveta Chiner-Oms, Álvaro Martínez-Lirola, Miguel Pérez-García, Felipe Gijón, Paloma Serrano, María Jesús Ruiz Romero, Laura Clotet Cuevas, Oscar Comas, Iñaki Bouza, Emilio Pérez-Lago, Laura García-de-Viedma, Darío Front Microbiol Microbiology Background: Mycobacterium tuberculosis (MTB) has limited ability to acquire variability. Analysis of its microevolution might help us to evaluate the pathways followed to acquire greater infective success. Whole-genome sequencing (WGS) in the analysis of the transmission of MTB has elucidated the magnitude of variability in MTB. Analysis of transmission currently depends on the identification of clusters, according to the threshold of variability (<5 SNPs) between isolates. Objective: We evaluated whether the acquisition of variability in MTB, was more frequent in situations which could favor it, namely intrapatient, prolonged infections or reactivations and interpatient transmissions involving multiple sequential hosts. Methods: We used WGS to analyze the accumulation of variability in sequential isolates from prolonged infections or translations from latency to reactivation. We then measured microevolution in transmission clusters with prolonged transmission time, high number of involved cases, simultaneous involvement of latency and active transmission. Results: Intrapatient and interpatient acquisition of variability was limited, within the ranges expected according to the thresholds of variability proposed, even though bursts of variability were observed. Conclusions: The thresholds of variability proposed for MTB seem to be valid in most circumstances, including those theoretically favoring acquisition of variability. Our data point to multifactorial modulation of microevolution, although further studies are necessary to elucidate the factors underlying this modulation. Frontiers Media S.A. 2018-01-19 /pmc/articles/PMC5780704/ /pubmed/29403447 http://dx.doi.org/10.3389/fmicb.2017.02661 Text en Copyright © 2018 Herranz, Pole, Ozere, Chiner-Oms, Martínez-Lirola, Pérez-García, Gijón, Serrano, Romero, Cuevas, Comas, Bouza, Pérez-Lago and García-de-Viedma. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Herranz, Marta
Pole, Ilva
Ozere, Iveta
Chiner-Oms, Álvaro
Martínez-Lirola, Miguel
Pérez-García, Felipe
Gijón, Paloma
Serrano, María Jesús Ruiz
Romero, Laura Clotet
Cuevas, Oscar
Comas, Iñaki
Bouza, Emilio
Pérez-Lago, Laura
García-de-Viedma, Darío
Mycobacterium tuberculosis Acquires Limited Genetic Diversity in Prolonged Infections, Reactivations and Transmissions Involving Multiple Hosts
title Mycobacterium tuberculosis Acquires Limited Genetic Diversity in Prolonged Infections, Reactivations and Transmissions Involving Multiple Hosts
title_full Mycobacterium tuberculosis Acquires Limited Genetic Diversity in Prolonged Infections, Reactivations and Transmissions Involving Multiple Hosts
title_fullStr Mycobacterium tuberculosis Acquires Limited Genetic Diversity in Prolonged Infections, Reactivations and Transmissions Involving Multiple Hosts
title_full_unstemmed Mycobacterium tuberculosis Acquires Limited Genetic Diversity in Prolonged Infections, Reactivations and Transmissions Involving Multiple Hosts
title_short Mycobacterium tuberculosis Acquires Limited Genetic Diversity in Prolonged Infections, Reactivations and Transmissions Involving Multiple Hosts
title_sort mycobacterium tuberculosis acquires limited genetic diversity in prolonged infections, reactivations and transmissions involving multiple hosts
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5780704/
https://www.ncbi.nlm.nih.gov/pubmed/29403447
http://dx.doi.org/10.3389/fmicb.2017.02661
work_keys_str_mv AT herranzmarta mycobacteriumtuberculosisacquireslimitedgeneticdiversityinprolongedinfectionsreactivationsandtransmissionsinvolvingmultiplehosts
AT poleilva mycobacteriumtuberculosisacquireslimitedgeneticdiversityinprolongedinfectionsreactivationsandtransmissionsinvolvingmultiplehosts
AT ozereiveta mycobacteriumtuberculosisacquireslimitedgeneticdiversityinprolongedinfectionsreactivationsandtransmissionsinvolvingmultiplehosts
AT chineromsalvaro mycobacteriumtuberculosisacquireslimitedgeneticdiversityinprolongedinfectionsreactivationsandtransmissionsinvolvingmultiplehosts
AT martinezlirolamiguel mycobacteriumtuberculosisacquireslimitedgeneticdiversityinprolongedinfectionsreactivationsandtransmissionsinvolvingmultiplehosts
AT perezgarciafelipe mycobacteriumtuberculosisacquireslimitedgeneticdiversityinprolongedinfectionsreactivationsandtransmissionsinvolvingmultiplehosts
AT gijonpaloma mycobacteriumtuberculosisacquireslimitedgeneticdiversityinprolongedinfectionsreactivationsandtransmissionsinvolvingmultiplehosts
AT serranomariajesusruiz mycobacteriumtuberculosisacquireslimitedgeneticdiversityinprolongedinfectionsreactivationsandtransmissionsinvolvingmultiplehosts
AT romerolauraclotet mycobacteriumtuberculosisacquireslimitedgeneticdiversityinprolongedinfectionsreactivationsandtransmissionsinvolvingmultiplehosts
AT cuevasoscar mycobacteriumtuberculosisacquireslimitedgeneticdiversityinprolongedinfectionsreactivationsandtransmissionsinvolvingmultiplehosts
AT comasinaki mycobacteriumtuberculosisacquireslimitedgeneticdiversityinprolongedinfectionsreactivationsandtransmissionsinvolvingmultiplehosts
AT bouzaemilio mycobacteriumtuberculosisacquireslimitedgeneticdiversityinprolongedinfectionsreactivationsandtransmissionsinvolvingmultiplehosts
AT perezlagolaura mycobacteriumtuberculosisacquireslimitedgeneticdiversityinprolongedinfectionsreactivationsandtransmissionsinvolvingmultiplehosts
AT garciadeviedmadario mycobacteriumtuberculosisacquireslimitedgeneticdiversityinprolongedinfectionsreactivationsandtransmissionsinvolvingmultiplehosts