Cargando…
Effects of miR-21 on proliferation and apoptosis in human gastric adenocarcinoma cells
The present study investigated the expression of miR-21 in MGC803 gastric cancer cells and its effects on Bcl-2 expression and cell proliferation, apoptosis, and invasion. In total 50 patients were recruited with gastric cancer who were admitted to the Henan Province People's Hospital. The samp...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5780787/ https://www.ncbi.nlm.nih.gov/pubmed/29403555 http://dx.doi.org/10.3892/ol.2017.6171 |
_version_ | 1783294808704417792 |
---|---|
author | Gu, Jun-Bao Bao, Xue-Bin Ma, Zhao |
author_facet | Gu, Jun-Bao Bao, Xue-Bin Ma, Zhao |
author_sort | Gu, Jun-Bao |
collection | PubMed |
description | The present study investigated the expression of miR-21 in MGC803 gastric cancer cells and its effects on Bcl-2 expression and cell proliferation, apoptosis, and invasion. In total 50 patients were recruited with gastric cancer who were admitted to the Henan Province People's Hospital. The samples of gastric cancer and the adjacent normal tissues were collected after surgery. We found that mRNA levels of miR-21 and Bcl-2 were significantly elevated in tumor tissues compared to control tissue. The expression of Bcl-2 protein was also elevated in cancerous tissue. This high expression of Bcl-2 was associated with clinical stage, lymph node metastasis, and tumor differentiation degree. Inhibition of miR-21 reduced the levels of miR-21 and Bcl-2 in MGC803 cells, and lowered cell proliferation and invasiveness. These results indicate that miR-21 and Bcl-2 may participate in the occurrence and development of gastric adenocarcinoma, suggesting their potential role as biomarkers and therapeutic targets. |
format | Online Article Text |
id | pubmed-5780787 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-57807872018-02-05 Effects of miR-21 on proliferation and apoptosis in human gastric adenocarcinoma cells Gu, Jun-Bao Bao, Xue-Bin Ma, Zhao Oncol Lett Articles The present study investigated the expression of miR-21 in MGC803 gastric cancer cells and its effects on Bcl-2 expression and cell proliferation, apoptosis, and invasion. In total 50 patients were recruited with gastric cancer who were admitted to the Henan Province People's Hospital. The samples of gastric cancer and the adjacent normal tissues were collected after surgery. We found that mRNA levels of miR-21 and Bcl-2 were significantly elevated in tumor tissues compared to control tissue. The expression of Bcl-2 protein was also elevated in cancerous tissue. This high expression of Bcl-2 was associated with clinical stage, lymph node metastasis, and tumor differentiation degree. Inhibition of miR-21 reduced the levels of miR-21 and Bcl-2 in MGC803 cells, and lowered cell proliferation and invasiveness. These results indicate that miR-21 and Bcl-2 may participate in the occurrence and development of gastric adenocarcinoma, suggesting their potential role as biomarkers and therapeutic targets. D.A. Spandidos 2018-01 2017-05-15 /pmc/articles/PMC5780787/ /pubmed/29403555 http://dx.doi.org/10.3892/ol.2017.6171 Text en Copyright: © Gu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Gu, Jun-Bao Bao, Xue-Bin Ma, Zhao Effects of miR-21 on proliferation and apoptosis in human gastric adenocarcinoma cells |
title | Effects of miR-21 on proliferation and apoptosis in human gastric adenocarcinoma cells |
title_full | Effects of miR-21 on proliferation and apoptosis in human gastric adenocarcinoma cells |
title_fullStr | Effects of miR-21 on proliferation and apoptosis in human gastric adenocarcinoma cells |
title_full_unstemmed | Effects of miR-21 on proliferation and apoptosis in human gastric adenocarcinoma cells |
title_short | Effects of miR-21 on proliferation and apoptosis in human gastric adenocarcinoma cells |
title_sort | effects of mir-21 on proliferation and apoptosis in human gastric adenocarcinoma cells |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5780787/ https://www.ncbi.nlm.nih.gov/pubmed/29403555 http://dx.doi.org/10.3892/ol.2017.6171 |
work_keys_str_mv | AT gujunbao effectsofmir21onproliferationandapoptosisinhumangastricadenocarcinomacells AT baoxuebin effectsofmir21onproliferationandapoptosisinhumangastricadenocarcinomacells AT mazhao effectsofmir21onproliferationandapoptosisinhumangastricadenocarcinomacells |