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Blood and alveolar lymphocyte subsets in pulmonary cytomegalovirus infection after lung transplantation

BACKGROUND: Cytomegalovirus (CMV) pneumonitis has been shown to be associated with lymphocytic alveolitis after lung transplantation. In the present study, we investigated a series of bronchoalveolar (BAL) and blood samples, collected in the absence of rejection or acute infectious episodes. in orde...

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Autores principales: Stéphan, François, Bernaudin, Jean-François, Cesari, Danielle, Fajac, Anne, Grenet, Dominique, Caubarrere, Isabelle, Stern, Marc
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2001
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC57810/
https://www.ncbi.nlm.nih.gov/pubmed/11602020
http://dx.doi.org/10.1186/1471-2334-1-15
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author Stéphan, François
Bernaudin, Jean-François
Cesari, Danielle
Fajac, Anne
Grenet, Dominique
Caubarrere, Isabelle
Stern, Marc
author_facet Stéphan, François
Bernaudin, Jean-François
Cesari, Danielle
Fajac, Anne
Grenet, Dominique
Caubarrere, Isabelle
Stern, Marc
author_sort Stéphan, François
collection PubMed
description BACKGROUND: Cytomegalovirus (CMV) pneumonitis has been shown to be associated with lymphocytic alveolitis after lung transplantation. In the present study, we investigated a series of bronchoalveolar (BAL) and blood samples, collected in the absence of rejection or acute infectious episodes. in order -1: to evaluate intra-alveolar cell population changes concomitant with CMV replication and -2: to reappraise the value of cell population analysis in the management of patients after lung transplantation. METHODS: We used flow cytometry to investigate modifications of lymphocyte subpopulations related to pulmonary cytomegalovirus infections in blood and BAL samples from a series of 13 lung transplant recipients. After exclusion of samples obtained during pulmonary rejection, bronchiolitis obliterans or acute bacterial infection, 48 blood and BAL samples were retained for analysis: 17 were CMV positive by shell-vial assay and 31 were CMV negative in blood and BAL. RESULTS: Our results demonstrate that pulmonary CMV infection is associated with a significant increase in the total lymphocyte population in BAL samples, but with minor modifications of the various lymphocyte subpopulations and a significantly higher absolute number of B lymphocytes in blood samples. CONCLUSIONS: Cytomegalovirus pulmonary infection is accompanied by only minor changes in BAL lymphocyte subpopulations. The study of BAL lymphocyte subpopulations therefore appears to be of limited clinical value in the diagnosis of pulmonary CMV infection. However, increased blood B-lymphocytes seems to be a clinical feature associated with CMV infection.
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spelling pubmed-578102001-10-15 Blood and alveolar lymphocyte subsets in pulmonary cytomegalovirus infection after lung transplantation Stéphan, François Bernaudin, Jean-François Cesari, Danielle Fajac, Anne Grenet, Dominique Caubarrere, Isabelle Stern, Marc BMC Infect Dis Research Article BACKGROUND: Cytomegalovirus (CMV) pneumonitis has been shown to be associated with lymphocytic alveolitis after lung transplantation. In the present study, we investigated a series of bronchoalveolar (BAL) and blood samples, collected in the absence of rejection or acute infectious episodes. in order -1: to evaluate intra-alveolar cell population changes concomitant with CMV replication and -2: to reappraise the value of cell population analysis in the management of patients after lung transplantation. METHODS: We used flow cytometry to investigate modifications of lymphocyte subpopulations related to pulmonary cytomegalovirus infections in blood and BAL samples from a series of 13 lung transplant recipients. After exclusion of samples obtained during pulmonary rejection, bronchiolitis obliterans or acute bacterial infection, 48 blood and BAL samples were retained for analysis: 17 were CMV positive by shell-vial assay and 31 were CMV negative in blood and BAL. RESULTS: Our results demonstrate that pulmonary CMV infection is associated with a significant increase in the total lymphocyte population in BAL samples, but with minor modifications of the various lymphocyte subpopulations and a significantly higher absolute number of B lymphocytes in blood samples. CONCLUSIONS: Cytomegalovirus pulmonary infection is accompanied by only minor changes in BAL lymphocyte subpopulations. The study of BAL lymphocyte subpopulations therefore appears to be of limited clinical value in the diagnosis of pulmonary CMV infection. However, increased blood B-lymphocytes seems to be a clinical feature associated with CMV infection. BioMed Central 2001-09-17 /pmc/articles/PMC57810/ /pubmed/11602020 http://dx.doi.org/10.1186/1471-2334-1-15 Text en Copyright © 2001 Stéphan et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
spellingShingle Research Article
Stéphan, François
Bernaudin, Jean-François
Cesari, Danielle
Fajac, Anne
Grenet, Dominique
Caubarrere, Isabelle
Stern, Marc
Blood and alveolar lymphocyte subsets in pulmonary cytomegalovirus infection after lung transplantation
title Blood and alveolar lymphocyte subsets in pulmonary cytomegalovirus infection after lung transplantation
title_full Blood and alveolar lymphocyte subsets in pulmonary cytomegalovirus infection after lung transplantation
title_fullStr Blood and alveolar lymphocyte subsets in pulmonary cytomegalovirus infection after lung transplantation
title_full_unstemmed Blood and alveolar lymphocyte subsets in pulmonary cytomegalovirus infection after lung transplantation
title_short Blood and alveolar lymphocyte subsets in pulmonary cytomegalovirus infection after lung transplantation
title_sort blood and alveolar lymphocyte subsets in pulmonary cytomegalovirus infection after lung transplantation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC57810/
https://www.ncbi.nlm.nih.gov/pubmed/11602020
http://dx.doi.org/10.1186/1471-2334-1-15
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