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Optimisation of high-quality total ribonucleic acid isolation from cartilaginous tissues for real-time polymerase chain reaction analysis

OBJECTIVES: Studies which consider the molecular mechanisms of degeneration and regeneration of cartilaginous tissues are seriously hampered by problematic ribonucleic acid (RNA) isolations due to low cell density and the dense, proteoglycan-rich extracellular matrix of cartilage. Proteoglycans tend...

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Detalles Bibliográficos
Autores principales:	Peeters, M., Huang, C. L., Vonk, L. A., Lu, Z. F., Bank, R. A., Helder, M. N., Doulabi, B. Zandieh
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	2016
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5782496/ https://www.ncbi.nlm.nih.gov/pubmed/27881439 http://dx.doi.org/10.1302/2046-3758.511.BJR-2016-0033.R3

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