The long noncoding RNA lncR492 inhibits neural differentiation of murine embryonic stem cells

RNA interference (RNAi) screens have been shown to be valuable to study embryonic stem cell (ESC) self-renewal and they have been successfully applied to identify coding as well as noncoding genes required for maintaining pluripotency. Here, we used an RNAi library targeting >640 long noncoding R...

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Autores principales: Winzi, Maria, Casas Vila, Nuria, Paszkowski-Rogacz, Maciej, Ding, Li, Noack, Svenja, Theis, Mirko, Butter, Falk, Buchholz, Frank
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5783419/
https://www.ncbi.nlm.nih.gov/pubmed/29364956
http://dx.doi.org/10.1371/journal.pone.0191682
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author Winzi, Maria
Casas Vila, Nuria
Paszkowski-Rogacz, Maciej
Ding, Li
Noack, Svenja
Theis, Mirko
Butter, Falk
Buchholz, Frank
author_facet Winzi, Maria
Casas Vila, Nuria
Paszkowski-Rogacz, Maciej
Ding, Li
Noack, Svenja
Theis, Mirko
Butter, Falk
Buchholz, Frank
author_sort Winzi, Maria
collection PubMed
description RNA interference (RNAi) screens have been shown to be valuable to study embryonic stem cell (ESC) self-renewal and they have been successfully applied to identify coding as well as noncoding genes required for maintaining pluripotency. Here, we used an RNAi library targeting >640 long noncoding RNAs (lncRNA) to probe for their role in early cell differentiation. Utilizing a Sox1-GFP ESC reporter cell line, we identified the lncRNA lncR492 as lineage-specific inhibitor of neuroectodermal differentiation. Molecular characterization showed that lncR492 interacts with the mRNA binding protein HuR and facilitates its inhibitory function by activation of Wnt signaling. Thus, lncRNAs modulate the fate decision of pluripotent stem cells.
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spelling pubmed-57834192018-02-08 The long noncoding RNA lncR492 inhibits neural differentiation of murine embryonic stem cells Winzi, Maria Casas Vila, Nuria Paszkowski-Rogacz, Maciej Ding, Li Noack, Svenja Theis, Mirko Butter, Falk Buchholz, Frank PLoS One Research Article RNA interference (RNAi) screens have been shown to be valuable to study embryonic stem cell (ESC) self-renewal and they have been successfully applied to identify coding as well as noncoding genes required for maintaining pluripotency. Here, we used an RNAi library targeting >640 long noncoding RNAs (lncRNA) to probe for their role in early cell differentiation. Utilizing a Sox1-GFP ESC reporter cell line, we identified the lncRNA lncR492 as lineage-specific inhibitor of neuroectodermal differentiation. Molecular characterization showed that lncR492 interacts with the mRNA binding protein HuR and facilitates its inhibitory function by activation of Wnt signaling. Thus, lncRNAs modulate the fate decision of pluripotent stem cells. Public Library of Science 2018-01-24 /pmc/articles/PMC5783419/ /pubmed/29364956 http://dx.doi.org/10.1371/journal.pone.0191682 Text en © 2018 Winzi et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Winzi, Maria
Casas Vila, Nuria
Paszkowski-Rogacz, Maciej
Ding, Li
Noack, Svenja
Theis, Mirko
Butter, Falk
Buchholz, Frank
The long noncoding RNA lncR492 inhibits neural differentiation of murine embryonic stem cells
title The long noncoding RNA lncR492 inhibits neural differentiation of murine embryonic stem cells
title_full The long noncoding RNA lncR492 inhibits neural differentiation of murine embryonic stem cells
title_fullStr The long noncoding RNA lncR492 inhibits neural differentiation of murine embryonic stem cells
title_full_unstemmed The long noncoding RNA lncR492 inhibits neural differentiation of murine embryonic stem cells
title_short The long noncoding RNA lncR492 inhibits neural differentiation of murine embryonic stem cells
title_sort long noncoding rna lncr492 inhibits neural differentiation of murine embryonic stem cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5783419/
https://www.ncbi.nlm.nih.gov/pubmed/29364956
http://dx.doi.org/10.1371/journal.pone.0191682
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