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Electrophysiological properties of strial pericytes and the effect of aspirin on pericyte K(+) channels
The present study was designed to investigate the electrophysiological properties of strial pericytes and the effect of aspirin on pericyte K(+) channels. Pericytes were identified by determining their morphological characteristics and using pericyte-associated immunofluorescence techniques. The ele...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5783500/ https://www.ncbi.nlm.nih.gov/pubmed/29257229 http://dx.doi.org/10.3892/mmr.2017.8194 |
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author | Liu, Yan-Hui Zhang, Zhi-Ping Wang, Yang Song, Jia Ma, Ke-Tao Si, Jun-Qiang Li, Li |
author_facet | Liu, Yan-Hui Zhang, Zhi-Ping Wang, Yang Song, Jia Ma, Ke-Tao Si, Jun-Qiang Li, Li |
author_sort | Liu, Yan-Hui |
collection | PubMed |
description | The present study was designed to investigate the electrophysiological properties of strial pericytes and the effect of aspirin on pericyte K(+) channels. Pericytes were identified by determining their morphological characteristics and using pericyte-associated immunofluorescence techniques. The electrophysiological properties of strial pericytes were observed with a whole-cell patch-clamp technique. Alterations in the outward current of cochlear pericytes in the stria vascularis of guinea pigs were examined following the application of K(+) channel retardants. The effects of aspirin on pericyte K(+) channels were also evaluated with the whole-cell patch-clamp technique. The results demonstrated that pericytes were desmin positive, and their nuclei were large and surrounded by a small proportion of the cytoplasm. Cytoplasmic processes gradually declined in size as branches grew parallel to the capillary axis. Thus, capillaries were surrounded by tips. The electrophysiological properties of the cochlear pericytes in the stria vascularis of guinea pigs were also determined. The membrane capacitance of the pericytes was 5.9±0.3 pF, while the membrane resistance and resting potential were 2.2±0.3 GΩ and −30.9±1.2 mV, respectively. The current densities of the pericytes (pA/pF) were 3.2±0.7, 10.6±1.0, 15.7±0.9 and 21.3±1.2 at command voltages of 0, +20, +40, and +60 mV, respectively. The K(+) channels were activated when the pericytes were within the range of −20 mV to +20 mV, particularly at 0 mV. The inhibition rates of the outward current of cochlear pericytes in the stria vascularis of the guinea pigs were determined by administering iberiotoxin (IBTX) and IBTX + 4-aminopyridine. Once the background leakage current was removed, the following inhibition rates were obtained with 3, 10, 30, 300 and 1,000 µmol/l aspirin: 20.8±4.8, 34.1±6.9, 48.2±6.7, 63.6±7.1 and 65.7±8.1%, respectively. The outward current of the cochlear pericytes in the stria vascularis was inhibited by aspirin with a half maximal inhibitory concentration of 24.5±4.5 µmol/l. The membranes of the pericytes in the stria vascularis are characterized by high-conductance calcium-activated K(+) (BK(Ca)) and voltage-dependent K(+) (K(V)) channels. The outward current of the cochlear pericytes in the stria vascularis of guinea pigs was inhibited by aspirin in a concentration-dependent manner. In addition, BK(Ca) and K(V) channels were inhibited by aspirin. |
format | Online Article Text |
id | pubmed-5783500 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-57835002018-02-12 Electrophysiological properties of strial pericytes and the effect of aspirin on pericyte K(+) channels Liu, Yan-Hui Zhang, Zhi-Ping Wang, Yang Song, Jia Ma, Ke-Tao Si, Jun-Qiang Li, Li Mol Med Rep Articles The present study was designed to investigate the electrophysiological properties of strial pericytes and the effect of aspirin on pericyte K(+) channels. Pericytes were identified by determining their morphological characteristics and using pericyte-associated immunofluorescence techniques. The electrophysiological properties of strial pericytes were observed with a whole-cell patch-clamp technique. Alterations in the outward current of cochlear pericytes in the stria vascularis of guinea pigs were examined following the application of K(+) channel retardants. The effects of aspirin on pericyte K(+) channels were also evaluated with the whole-cell patch-clamp technique. The results demonstrated that pericytes were desmin positive, and their nuclei were large and surrounded by a small proportion of the cytoplasm. Cytoplasmic processes gradually declined in size as branches grew parallel to the capillary axis. Thus, capillaries were surrounded by tips. The electrophysiological properties of the cochlear pericytes in the stria vascularis of guinea pigs were also determined. The membrane capacitance of the pericytes was 5.9±0.3 pF, while the membrane resistance and resting potential were 2.2±0.3 GΩ and −30.9±1.2 mV, respectively. The current densities of the pericytes (pA/pF) were 3.2±0.7, 10.6±1.0, 15.7±0.9 and 21.3±1.2 at command voltages of 0, +20, +40, and +60 mV, respectively. The K(+) channels were activated when the pericytes were within the range of −20 mV to +20 mV, particularly at 0 mV. The inhibition rates of the outward current of cochlear pericytes in the stria vascularis of the guinea pigs were determined by administering iberiotoxin (IBTX) and IBTX + 4-aminopyridine. Once the background leakage current was removed, the following inhibition rates were obtained with 3, 10, 30, 300 and 1,000 µmol/l aspirin: 20.8±4.8, 34.1±6.9, 48.2±6.7, 63.6±7.1 and 65.7±8.1%, respectively. The outward current of the cochlear pericytes in the stria vascularis was inhibited by aspirin with a half maximal inhibitory concentration of 24.5±4.5 µmol/l. The membranes of the pericytes in the stria vascularis are characterized by high-conductance calcium-activated K(+) (BK(Ca)) and voltage-dependent K(+) (K(V)) channels. The outward current of the cochlear pericytes in the stria vascularis of guinea pigs was inhibited by aspirin in a concentration-dependent manner. In addition, BK(Ca) and K(V) channels were inhibited by aspirin. D.A. Spandidos 2018-02 2017-12-06 /pmc/articles/PMC5783500/ /pubmed/29257229 http://dx.doi.org/10.3892/mmr.2017.8194 Text en Copyright: © Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Liu, Yan-Hui Zhang, Zhi-Ping Wang, Yang Song, Jia Ma, Ke-Tao Si, Jun-Qiang Li, Li Electrophysiological properties of strial pericytes and the effect of aspirin on pericyte K(+) channels |
title | Electrophysiological properties of strial pericytes and the effect of aspirin on pericyte K(+) channels |
title_full | Electrophysiological properties of strial pericytes and the effect of aspirin on pericyte K(+) channels |
title_fullStr | Electrophysiological properties of strial pericytes and the effect of aspirin on pericyte K(+) channels |
title_full_unstemmed | Electrophysiological properties of strial pericytes and the effect of aspirin on pericyte K(+) channels |
title_short | Electrophysiological properties of strial pericytes and the effect of aspirin on pericyte K(+) channels |
title_sort | electrophysiological properties of strial pericytes and the effect of aspirin on pericyte k(+) channels |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5783500/ https://www.ncbi.nlm.nih.gov/pubmed/29257229 http://dx.doi.org/10.3892/mmr.2017.8194 |
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