Electroporation-delivered fluorescent protein biosensors for probing molecular activities in cells without genetic encoding()

Fluorescent protein biosensors are typically implemented via genetic encoding which makes the examination of scarce cell samples impractical. By directly delivering the protein form of the biosensor into cells using electroporation, we detected intracellular molecular activity with the sample size d...

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Detalles Bibliográficos
Autores principales: Sun, Chen, Ouyang, Mingxing, Cao, Zhenning, Ma, Sai, Alqublan, Hamzeh, Sriranganathan, Nammalwar, Wang, Yingxiao, Lu, Chang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5783554/
https://www.ncbi.nlm.nih.gov/pubmed/25133322
http://dx.doi.org/10.1039/c4cc04730c
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author Sun, Chen
Ouyang, Mingxing
Cao, Zhenning
Ma, Sai
Alqublan, Hamzeh
Sriranganathan, Nammalwar
Wang, Yingxiao
Lu, Chang
author_facet Sun, Chen
Ouyang, Mingxing
Cao, Zhenning
Ma, Sai
Alqublan, Hamzeh
Sriranganathan, Nammalwar
Wang, Yingxiao
Lu, Chang
author_sort Sun, Chen
collection PubMed
description Fluorescent protein biosensors are typically implemented via genetic encoding which makes the examination of scarce cell samples impractical. By directly delivering the protein form of the biosensor into cells using electroporation, we detected intracellular molecular activity with the sample size down to ~100 cells with high spatiotemporal resolution.
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spelling pubmed-57835542018-01-24 Electroporation-delivered fluorescent protein biosensors for probing molecular activities in cells without genetic encoding() Sun, Chen Ouyang, Mingxing Cao, Zhenning Ma, Sai Alqublan, Hamzeh Sriranganathan, Nammalwar Wang, Yingxiao Lu, Chang Chem Commun (Camb) Article Fluorescent protein biosensors are typically implemented via genetic encoding which makes the examination of scarce cell samples impractical. By directly delivering the protein form of the biosensor into cells using electroporation, we detected intracellular molecular activity with the sample size down to ~100 cells with high spatiotemporal resolution. 2014-10-09 /pmc/articles/PMC5783554/ /pubmed/25133322 http://dx.doi.org/10.1039/c4cc04730c Text en This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence (http://creativecommons.org/licenses/by-nc/3.0/) .
spellingShingle Article
Sun, Chen
Ouyang, Mingxing
Cao, Zhenning
Ma, Sai
Alqublan, Hamzeh
Sriranganathan, Nammalwar
Wang, Yingxiao
Lu, Chang
Electroporation-delivered fluorescent protein biosensors for probing molecular activities in cells without genetic encoding()
title Electroporation-delivered fluorescent protein biosensors for probing molecular activities in cells without genetic encoding()
title_full Electroporation-delivered fluorescent protein biosensors for probing molecular activities in cells without genetic encoding()
title_fullStr Electroporation-delivered fluorescent protein biosensors for probing molecular activities in cells without genetic encoding()
title_full_unstemmed Electroporation-delivered fluorescent protein biosensors for probing molecular activities in cells without genetic encoding()
title_short Electroporation-delivered fluorescent protein biosensors for probing molecular activities in cells without genetic encoding()
title_sort electroporation-delivered fluorescent protein biosensors for probing molecular activities in cells without genetic encoding()
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5783554/
https://www.ncbi.nlm.nih.gov/pubmed/25133322
http://dx.doi.org/10.1039/c4cc04730c
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