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Knockdown of HIP1 expression promotes ligand-induced endocytosis of EGFR in HeLa cells
Huntington-interacting protein 1 (HIP1) is associated with various tumor types; however, its precise functions in tumor cells are unclear. In this study, the effects of HIP1 on the degradation of EGFR, which have important roles in carcinogenesis after EGF stimulation, were examined. After screening...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5783582/ https://www.ncbi.nlm.nih.gov/pubmed/29039605 http://dx.doi.org/10.3892/or.2017.6025 |
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author | Li, Dan Chen, Fenglin Ding, Jian Lin, Na Li, Zonghai Wang, Xiaozhong |
author_facet | Li, Dan Chen, Fenglin Ding, Jian Lin, Na Li, Zonghai Wang, Xiaozhong |
author_sort | Li, Dan |
collection | PubMed |
description | Huntington-interacting protein 1 (HIP1) is associated with various tumor types; however, its precise functions in tumor cells are unclear. In this study, the effects of HIP1 on the degradation of EGFR, which have important roles in carcinogenesis after EGF stimulation, were examined. After screening 17 cell lines, the coexpression of HIP1 and EGFR was detected in HeLa cells. Accordingly, the expression of HIP1 was knocked down in HeLa cells using various HIP1 siRNA sequences. The endocytosis of EGFR and localization of clathrin in HeLa cells were examined after stimulation by EGF at various concentrations (i.e., 1.5 and 100 ng/ml). After HIP1 expression was blocked by siRNAs, EGFR endocytosis was accelerated and this effect was dependent on the EGF concentration. This endocytosis was colocalized with clathrin expression. These findings indicate that the inhibition of HIP1 can accelerate the endocytosis and degradation of EGFR. Furthermore, they suggest that HIP1 is a potential therapeutic target for various cancer types, particularly those with high EGFR expression, but further research is needed to examine this hypothesis. |
format | Online Article Text |
id | pubmed-5783582 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-57835822018-02-12 Knockdown of HIP1 expression promotes ligand-induced endocytosis of EGFR in HeLa cells Li, Dan Chen, Fenglin Ding, Jian Lin, Na Li, Zonghai Wang, Xiaozhong Oncol Rep Articles Huntington-interacting protein 1 (HIP1) is associated with various tumor types; however, its precise functions in tumor cells are unclear. In this study, the effects of HIP1 on the degradation of EGFR, which have important roles in carcinogenesis after EGF stimulation, were examined. After screening 17 cell lines, the coexpression of HIP1 and EGFR was detected in HeLa cells. Accordingly, the expression of HIP1 was knocked down in HeLa cells using various HIP1 siRNA sequences. The endocytosis of EGFR and localization of clathrin in HeLa cells were examined after stimulation by EGF at various concentrations (i.e., 1.5 and 100 ng/ml). After HIP1 expression was blocked by siRNAs, EGFR endocytosis was accelerated and this effect was dependent on the EGF concentration. This endocytosis was colocalized with clathrin expression. These findings indicate that the inhibition of HIP1 can accelerate the endocytosis and degradation of EGFR. Furthermore, they suggest that HIP1 is a potential therapeutic target for various cancer types, particularly those with high EGFR expression, but further research is needed to examine this hypothesis. D.A. Spandidos 2017-12 2017-10-12 /pmc/articles/PMC5783582/ /pubmed/29039605 http://dx.doi.org/10.3892/or.2017.6025 Text en Copyright: © Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Li, Dan Chen, Fenglin Ding, Jian Lin, Na Li, Zonghai Wang, Xiaozhong Knockdown of HIP1 expression promotes ligand-induced endocytosis of EGFR in HeLa cells |
title | Knockdown of HIP1 expression promotes ligand-induced endocytosis of EGFR in HeLa cells |
title_full | Knockdown of HIP1 expression promotes ligand-induced endocytosis of EGFR in HeLa cells |
title_fullStr | Knockdown of HIP1 expression promotes ligand-induced endocytosis of EGFR in HeLa cells |
title_full_unstemmed | Knockdown of HIP1 expression promotes ligand-induced endocytosis of EGFR in HeLa cells |
title_short | Knockdown of HIP1 expression promotes ligand-induced endocytosis of EGFR in HeLa cells |
title_sort | knockdown of hip1 expression promotes ligand-induced endocytosis of egfr in hela cells |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5783582/ https://www.ncbi.nlm.nih.gov/pubmed/29039605 http://dx.doi.org/10.3892/or.2017.6025 |
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