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High diversity and abundance of cultivable tetracycline-resistant bacteria in soil following pig manure application

By performing a microcosm experiment mimicking fertilization, we assessed the dynamic distribution of tetracycline-resistant bacteria (TRB) and corresponding tetracycline resistance genes (TRGs) from pig manure (PM) to the fertilized soil, by culture-dependent methods and PCR detection. Cultivable T...

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Autores principales: Kang, Yijun, Li, Qing, Yin, Zhifeng, Shen, Min, Zhao, Haitao, Bai, Yanchao, Mei, Lijuan, Hu, Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5784163/
https://www.ncbi.nlm.nih.gov/pubmed/29367695
http://dx.doi.org/10.1038/s41598-018-20050-8
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author Kang, Yijun
Li, Qing
Yin, Zhifeng
Shen, Min
Zhao, Haitao
Bai, Yanchao
Mei, Lijuan
Hu, Jian
author_facet Kang, Yijun
Li, Qing
Yin, Zhifeng
Shen, Min
Zhao, Haitao
Bai, Yanchao
Mei, Lijuan
Hu, Jian
author_sort Kang, Yijun
collection PubMed
description By performing a microcosm experiment mimicking fertilization, we assessed the dynamic distribution of tetracycline-resistant bacteria (TRB) and corresponding tetracycline resistance genes (TRGs) from pig manure (PM) to the fertilized soil, by culture-dependent methods and PCR detection. Cultivable TRB were most abundant in PM, followed by fertilized soil and unfertilized soil. By restriction fragment length polymorphism (RFLP) analysis, TRB were assigned to 29, 20, and 153 operational taxonomic units (OTUs) in PM, unfertilized soil, and fertilized soil, respectively. After identification, they were further grouped into 19, 12, and 62 species, showing an enhanced diversity of cultivable TRB in the soil following PM application. The proportions of potentially pathogenic TRB in fertilized soil decreased by 69.35% and 41.92% compared with PM and unfertilized soil. Bacillus cereus was likely widely distributed TRB under various environments, and Rhodococcus erythropolis and Acinetobacter sp. probably spread from PM to the soil via fertilization. Meanwhile, tetL was the most common efflux pump gene in both unfertilized and fertilized soils relative to PM; tetB(P) and tet36 were common in PM, whereas tetO was predominant in unfertilized and fertilized soil samples. Sequencing indicated that over 65% of randomly selected TRB in fertilized soil with acquired resistance derived from PM.
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spelling pubmed-57841632018-02-07 High diversity and abundance of cultivable tetracycline-resistant bacteria in soil following pig manure application Kang, Yijun Li, Qing Yin, Zhifeng Shen, Min Zhao, Haitao Bai, Yanchao Mei, Lijuan Hu, Jian Sci Rep Article By performing a microcosm experiment mimicking fertilization, we assessed the dynamic distribution of tetracycline-resistant bacteria (TRB) and corresponding tetracycline resistance genes (TRGs) from pig manure (PM) to the fertilized soil, by culture-dependent methods and PCR detection. Cultivable TRB were most abundant in PM, followed by fertilized soil and unfertilized soil. By restriction fragment length polymorphism (RFLP) analysis, TRB were assigned to 29, 20, and 153 operational taxonomic units (OTUs) in PM, unfertilized soil, and fertilized soil, respectively. After identification, they were further grouped into 19, 12, and 62 species, showing an enhanced diversity of cultivable TRB in the soil following PM application. The proportions of potentially pathogenic TRB in fertilized soil decreased by 69.35% and 41.92% compared with PM and unfertilized soil. Bacillus cereus was likely widely distributed TRB under various environments, and Rhodococcus erythropolis and Acinetobacter sp. probably spread from PM to the soil via fertilization. Meanwhile, tetL was the most common efflux pump gene in both unfertilized and fertilized soils relative to PM; tetB(P) and tet36 were common in PM, whereas tetO was predominant in unfertilized and fertilized soil samples. Sequencing indicated that over 65% of randomly selected TRB in fertilized soil with acquired resistance derived from PM. Nature Publishing Group UK 2018-01-24 /pmc/articles/PMC5784163/ /pubmed/29367695 http://dx.doi.org/10.1038/s41598-018-20050-8 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Kang, Yijun
Li, Qing
Yin, Zhifeng
Shen, Min
Zhao, Haitao
Bai, Yanchao
Mei, Lijuan
Hu, Jian
High diversity and abundance of cultivable tetracycline-resistant bacteria in soil following pig manure application
title High diversity and abundance of cultivable tetracycline-resistant bacteria in soil following pig manure application
title_full High diversity and abundance of cultivable tetracycline-resistant bacteria in soil following pig manure application
title_fullStr High diversity and abundance of cultivable tetracycline-resistant bacteria in soil following pig manure application
title_full_unstemmed High diversity and abundance of cultivable tetracycline-resistant bacteria in soil following pig manure application
title_short High diversity and abundance of cultivable tetracycline-resistant bacteria in soil following pig manure application
title_sort high diversity and abundance of cultivable tetracycline-resistant bacteria in soil following pig manure application
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5784163/
https://www.ncbi.nlm.nih.gov/pubmed/29367695
http://dx.doi.org/10.1038/s41598-018-20050-8
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