Cargando…
Mounting of Biomaterials for Use in Ophthalmic Cell Therapies
When used as scaffolds for cell therapies, biomaterials often present basic handling and logistical problems for scientists and surgeons alike. The quest for an appropriate mounting device for biomaterials is therefore a significant and common problem. In this review, we provide a detailed overview...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5784520/ https://www.ncbi.nlm.nih.gov/pubmed/29338382 http://dx.doi.org/10.1177/0963689717723638 |
_version_ | 1783295457871527936 |
---|---|
author | Harkin, Damien G. Dunphy, Siobhan E. Shadforth, Audra M. A. Dawson, Rebecca A. Walshe, Jennifer Zakaria, Nadia |
author_facet | Harkin, Damien G. Dunphy, Siobhan E. Shadforth, Audra M. A. Dawson, Rebecca A. Walshe, Jennifer Zakaria, Nadia |
author_sort | Harkin, Damien G. |
collection | PubMed |
description | When used as scaffolds for cell therapies, biomaterials often present basic handling and logistical problems for scientists and surgeons alike. The quest for an appropriate mounting device for biomaterials is therefore a significant and common problem. In this review, we provide a detailed overview of the factors to consider when choosing an appropriate mounting device including those experienced during cell culture, quality assurance, and surgery. By way of example, we draw upon our combined experience in developing epithelial cell therapies for the treatment of eye diseases. We discuss commercially available options for achieving required goals and provide a detailed analysis of 4 experimental designs developed within our respective laboratories in Australia, the United Kingdom, and Belgium. |
format | Online Article Text |
id | pubmed-5784520 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-57845202018-01-30 Mounting of Biomaterials for Use in Ophthalmic Cell Therapies Harkin, Damien G. Dunphy, Siobhan E. Shadforth, Audra M. A. Dawson, Rebecca A. Walshe, Jennifer Zakaria, Nadia Cell Transplant Original Articles When used as scaffolds for cell therapies, biomaterials often present basic handling and logistical problems for scientists and surgeons alike. The quest for an appropriate mounting device for biomaterials is therefore a significant and common problem. In this review, we provide a detailed overview of the factors to consider when choosing an appropriate mounting device including those experienced during cell culture, quality assurance, and surgery. By way of example, we draw upon our combined experience in developing epithelial cell therapies for the treatment of eye diseases. We discuss commercially available options for achieving required goals and provide a detailed analysis of 4 experimental designs developed within our respective laboratories in Australia, the United Kingdom, and Belgium. SAGE Publications 2018-01-16 2017-11 /pmc/articles/PMC5784520/ /pubmed/29338382 http://dx.doi.org/10.1177/0963689717723638 Text en © The Author(s) 2017 http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Original Articles Harkin, Damien G. Dunphy, Siobhan E. Shadforth, Audra M. A. Dawson, Rebecca A. Walshe, Jennifer Zakaria, Nadia Mounting of Biomaterials for Use in Ophthalmic Cell Therapies |
title | Mounting of Biomaterials for Use in Ophthalmic Cell Therapies |
title_full | Mounting of Biomaterials for Use in Ophthalmic Cell Therapies |
title_fullStr | Mounting of Biomaterials for Use in Ophthalmic Cell Therapies |
title_full_unstemmed | Mounting of Biomaterials for Use in Ophthalmic Cell Therapies |
title_short | Mounting of Biomaterials for Use in Ophthalmic Cell Therapies |
title_sort | mounting of biomaterials for use in ophthalmic cell therapies |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5784520/ https://www.ncbi.nlm.nih.gov/pubmed/29338382 http://dx.doi.org/10.1177/0963689717723638 |
work_keys_str_mv | AT harkindamieng mountingofbiomaterialsforuseinophthalmiccelltherapies AT dunphysiobhane mountingofbiomaterialsforuseinophthalmiccelltherapies AT shadforthaudrama mountingofbiomaterialsforuseinophthalmiccelltherapies AT dawsonrebeccaa mountingofbiomaterialsforuseinophthalmiccelltherapies AT walshejennifer mountingofbiomaterialsforuseinophthalmiccelltherapies AT zakarianadia mountingofbiomaterialsforuseinophthalmiccelltherapies |