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Eukaryotes in the gut microbiota in myalgic encephalomyelitis/chronic fatigue syndrome

Patients with myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) often suffer from gastrointestinal symptoms and many are diagnosed with irritable bowel syndrome (IBS). Previous studies, including from our laboratory, have demonstrated that the ME/CFS gut bacterial composition is altered an...

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Autores principales: Mandarano, Alexandra H., Giloteaux, Ludovic, Keller, Betsy A., Levine, Susan M., Hanson, Maureen R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5784577/
https://www.ncbi.nlm.nih.gov/pubmed/29375937
http://dx.doi.org/10.7717/peerj.4282
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author Mandarano, Alexandra H.
Giloteaux, Ludovic
Keller, Betsy A.
Levine, Susan M.
Hanson, Maureen R.
author_facet Mandarano, Alexandra H.
Giloteaux, Ludovic
Keller, Betsy A.
Levine, Susan M.
Hanson, Maureen R.
author_sort Mandarano, Alexandra H.
collection PubMed
description Patients with myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) often suffer from gastrointestinal symptoms and many are diagnosed with irritable bowel syndrome (IBS). Previous studies, including from our laboratory, have demonstrated that the ME/CFS gut bacterial composition is altered and less diverse when compared to healthy individuals. Patients have increased biomarkers of inflammation and leaky gut syndrome. To further investigate dysbiosis in the ME/CFS gut microbiome, we sought to characterize the eukaryotes present in the gut of 49 individuals with ME/CFS and 39 healthy controls. Using 18S rRNA sequencing, we have identified eukaryotes in stool samples of 17 healthy individuals and 17 ME/CFS patients. Our analysis demonstrates a small, nonsignificant decrease in eukaryotic diversity in ME/CFS patients compared to healthy individuals. In addition, ME/CFS patients show a nonsignificant increase in the ratio of fungal phyla Basidiomycota to Ascomycota, which is consistent with ongoing inflammation in ME/CFS. We did not identify specific eukaryotic taxa that are associated with ME/CFS disease status.
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spelling pubmed-57845772018-01-26 Eukaryotes in the gut microbiota in myalgic encephalomyelitis/chronic fatigue syndrome Mandarano, Alexandra H. Giloteaux, Ludovic Keller, Betsy A. Levine, Susan M. Hanson, Maureen R. PeerJ Microbiology Patients with myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) often suffer from gastrointestinal symptoms and many are diagnosed with irritable bowel syndrome (IBS). Previous studies, including from our laboratory, have demonstrated that the ME/CFS gut bacterial composition is altered and less diverse when compared to healthy individuals. Patients have increased biomarkers of inflammation and leaky gut syndrome. To further investigate dysbiosis in the ME/CFS gut microbiome, we sought to characterize the eukaryotes present in the gut of 49 individuals with ME/CFS and 39 healthy controls. Using 18S rRNA sequencing, we have identified eukaryotes in stool samples of 17 healthy individuals and 17 ME/CFS patients. Our analysis demonstrates a small, nonsignificant decrease in eukaryotic diversity in ME/CFS patients compared to healthy individuals. In addition, ME/CFS patients show a nonsignificant increase in the ratio of fungal phyla Basidiomycota to Ascomycota, which is consistent with ongoing inflammation in ME/CFS. We did not identify specific eukaryotic taxa that are associated with ME/CFS disease status. PeerJ Inc. 2018-01-22 /pmc/articles/PMC5784577/ /pubmed/29375937 http://dx.doi.org/10.7717/peerj.4282 Text en ©2018 Mandarano et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Microbiology
Mandarano, Alexandra H.
Giloteaux, Ludovic
Keller, Betsy A.
Levine, Susan M.
Hanson, Maureen R.
Eukaryotes in the gut microbiota in myalgic encephalomyelitis/chronic fatigue syndrome
title Eukaryotes in the gut microbiota in myalgic encephalomyelitis/chronic fatigue syndrome
title_full Eukaryotes in the gut microbiota in myalgic encephalomyelitis/chronic fatigue syndrome
title_fullStr Eukaryotes in the gut microbiota in myalgic encephalomyelitis/chronic fatigue syndrome
title_full_unstemmed Eukaryotes in the gut microbiota in myalgic encephalomyelitis/chronic fatigue syndrome
title_short Eukaryotes in the gut microbiota in myalgic encephalomyelitis/chronic fatigue syndrome
title_sort eukaryotes in the gut microbiota in myalgic encephalomyelitis/chronic fatigue syndrome
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5784577/
https://www.ncbi.nlm.nih.gov/pubmed/29375937
http://dx.doi.org/10.7717/peerj.4282
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