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Identification of membrane-associated proteins with pathogenic potential expressed by Corynebacterium pseudotuberculosis grown in animal serum

OBJECTIVE: Previous works defining antigens that might be used as vaccine targets against Corynebacterium pseudotuberculosis, which is the causative agent of sheep and goat caseous lymphadenitis, have focused on secreted proteins produced in a chemically defined culture media. Considering that such...

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Autores principales: Raynal, José Tadeu, Bastos, Bruno Lopes, Vilas-Boas, Priscilla Carolinne Bagano, Sousa, Thiago de Jesus, Costa-Silva, Marcos, de Sá, Maria da Conceição Aquino, Portela, Ricardo Wagner, Moura-Costa, Lília Ferreira, Azevedo, Vasco, Meyer, Roberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5784612/
https://www.ncbi.nlm.nih.gov/pubmed/29368627
http://dx.doi.org/10.1186/s13104-018-3180-5
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author Raynal, José Tadeu
Bastos, Bruno Lopes
Vilas-Boas, Priscilla Carolinne Bagano
Sousa, Thiago de Jesus
Costa-Silva, Marcos
de Sá, Maria da Conceição Aquino
Portela, Ricardo Wagner
Moura-Costa, Lília Ferreira
Azevedo, Vasco
Meyer, Roberto
author_facet Raynal, José Tadeu
Bastos, Bruno Lopes
Vilas-Boas, Priscilla Carolinne Bagano
Sousa, Thiago de Jesus
Costa-Silva, Marcos
de Sá, Maria da Conceição Aquino
Portela, Ricardo Wagner
Moura-Costa, Lília Ferreira
Azevedo, Vasco
Meyer, Roberto
author_sort Raynal, José Tadeu
collection PubMed
description OBJECTIVE: Previous works defining antigens that might be used as vaccine targets against Corynebacterium pseudotuberculosis, which is the causative agent of sheep and goat caseous lymphadenitis, have focused on secreted proteins produced in a chemically defined culture media. Considering that such antigens might not reflect the repertoire of proteins expressed during infection conditions, this experiment aimed to investigate the membrane-associated proteins with pathogenic potential expressed by C. pseudotuberculosis grown directly in animal serum. RESULTS: Its membrane-associated proteins have been extracted using an organic solvent enrichment methodology, followed by LC–MS/MS and bioinformatics analysis for protein identification and classification. The results revealed 22 membrane-associated proteins characterized as potentially pathogenic. An interaction network analysis indicated that the four potentially pathogenic proteins ciuA, fagA, OppA4 and OppCD were biologically connected within two distinct network pathways, which were both associated with the ABC Transporters KEGG pathway. These results suggest that C. pseudotuberculosis pathogenesis might be associated with the transport and uptake of nutrients; other seven identified potentially pathogenic membrane proteins also suggest that pathogenesis might involve events of bacterial resistance and adhesion. The proteins herein reported potentially reflect part of the protein repertoire expressed during real infection conditions and might be tested as vaccine antigens. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13104-018-3180-5) contains supplementary material, which is available to authorized users.
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spelling pubmed-57846122018-02-07 Identification of membrane-associated proteins with pathogenic potential expressed by Corynebacterium pseudotuberculosis grown in animal serum Raynal, José Tadeu Bastos, Bruno Lopes Vilas-Boas, Priscilla Carolinne Bagano Sousa, Thiago de Jesus Costa-Silva, Marcos de Sá, Maria da Conceição Aquino Portela, Ricardo Wagner Moura-Costa, Lília Ferreira Azevedo, Vasco Meyer, Roberto BMC Res Notes Research Note OBJECTIVE: Previous works defining antigens that might be used as vaccine targets against Corynebacterium pseudotuberculosis, which is the causative agent of sheep and goat caseous lymphadenitis, have focused on secreted proteins produced in a chemically defined culture media. Considering that such antigens might not reflect the repertoire of proteins expressed during infection conditions, this experiment aimed to investigate the membrane-associated proteins with pathogenic potential expressed by C. pseudotuberculosis grown directly in animal serum. RESULTS: Its membrane-associated proteins have been extracted using an organic solvent enrichment methodology, followed by LC–MS/MS and bioinformatics analysis for protein identification and classification. The results revealed 22 membrane-associated proteins characterized as potentially pathogenic. An interaction network analysis indicated that the four potentially pathogenic proteins ciuA, fagA, OppA4 and OppCD were biologically connected within two distinct network pathways, which were both associated with the ABC Transporters KEGG pathway. These results suggest that C. pseudotuberculosis pathogenesis might be associated with the transport and uptake of nutrients; other seven identified potentially pathogenic membrane proteins also suggest that pathogenesis might involve events of bacterial resistance and adhesion. The proteins herein reported potentially reflect part of the protein repertoire expressed during real infection conditions and might be tested as vaccine antigens. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13104-018-3180-5) contains supplementary material, which is available to authorized users. BioMed Central 2018-01-25 /pmc/articles/PMC5784612/ /pubmed/29368627 http://dx.doi.org/10.1186/s13104-018-3180-5 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Note
Raynal, José Tadeu
Bastos, Bruno Lopes
Vilas-Boas, Priscilla Carolinne Bagano
Sousa, Thiago de Jesus
Costa-Silva, Marcos
de Sá, Maria da Conceição Aquino
Portela, Ricardo Wagner
Moura-Costa, Lília Ferreira
Azevedo, Vasco
Meyer, Roberto
Identification of membrane-associated proteins with pathogenic potential expressed by Corynebacterium pseudotuberculosis grown in animal serum
title Identification of membrane-associated proteins with pathogenic potential expressed by Corynebacterium pseudotuberculosis grown in animal serum
title_full Identification of membrane-associated proteins with pathogenic potential expressed by Corynebacterium pseudotuberculosis grown in animal serum
title_fullStr Identification of membrane-associated proteins with pathogenic potential expressed by Corynebacterium pseudotuberculosis grown in animal serum
title_full_unstemmed Identification of membrane-associated proteins with pathogenic potential expressed by Corynebacterium pseudotuberculosis grown in animal serum
title_short Identification of membrane-associated proteins with pathogenic potential expressed by Corynebacterium pseudotuberculosis grown in animal serum
title_sort identification of membrane-associated proteins with pathogenic potential expressed by corynebacterium pseudotuberculosis grown in animal serum
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5784612/
https://www.ncbi.nlm.nih.gov/pubmed/29368627
http://dx.doi.org/10.1186/s13104-018-3180-5
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