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Identification of membrane-associated proteins with pathogenic potential expressed by Corynebacterium pseudotuberculosis grown in animal serum
OBJECTIVE: Previous works defining antigens that might be used as vaccine targets against Corynebacterium pseudotuberculosis, which is the causative agent of sheep and goat caseous lymphadenitis, have focused on secreted proteins produced in a chemically defined culture media. Considering that such...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5784612/ https://www.ncbi.nlm.nih.gov/pubmed/29368627 http://dx.doi.org/10.1186/s13104-018-3180-5 |
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author | Raynal, José Tadeu Bastos, Bruno Lopes Vilas-Boas, Priscilla Carolinne Bagano Sousa, Thiago de Jesus Costa-Silva, Marcos de Sá, Maria da Conceição Aquino Portela, Ricardo Wagner Moura-Costa, Lília Ferreira Azevedo, Vasco Meyer, Roberto |
author_facet | Raynal, José Tadeu Bastos, Bruno Lopes Vilas-Boas, Priscilla Carolinne Bagano Sousa, Thiago de Jesus Costa-Silva, Marcos de Sá, Maria da Conceição Aquino Portela, Ricardo Wagner Moura-Costa, Lília Ferreira Azevedo, Vasco Meyer, Roberto |
author_sort | Raynal, José Tadeu |
collection | PubMed |
description | OBJECTIVE: Previous works defining antigens that might be used as vaccine targets against Corynebacterium pseudotuberculosis, which is the causative agent of sheep and goat caseous lymphadenitis, have focused on secreted proteins produced in a chemically defined culture media. Considering that such antigens might not reflect the repertoire of proteins expressed during infection conditions, this experiment aimed to investigate the membrane-associated proteins with pathogenic potential expressed by C. pseudotuberculosis grown directly in animal serum. RESULTS: Its membrane-associated proteins have been extracted using an organic solvent enrichment methodology, followed by LC–MS/MS and bioinformatics analysis for protein identification and classification. The results revealed 22 membrane-associated proteins characterized as potentially pathogenic. An interaction network analysis indicated that the four potentially pathogenic proteins ciuA, fagA, OppA4 and OppCD were biologically connected within two distinct network pathways, which were both associated with the ABC Transporters KEGG pathway. These results suggest that C. pseudotuberculosis pathogenesis might be associated with the transport and uptake of nutrients; other seven identified potentially pathogenic membrane proteins also suggest that pathogenesis might involve events of bacterial resistance and adhesion. The proteins herein reported potentially reflect part of the protein repertoire expressed during real infection conditions and might be tested as vaccine antigens. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13104-018-3180-5) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5784612 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-57846122018-02-07 Identification of membrane-associated proteins with pathogenic potential expressed by Corynebacterium pseudotuberculosis grown in animal serum Raynal, José Tadeu Bastos, Bruno Lopes Vilas-Boas, Priscilla Carolinne Bagano Sousa, Thiago de Jesus Costa-Silva, Marcos de Sá, Maria da Conceição Aquino Portela, Ricardo Wagner Moura-Costa, Lília Ferreira Azevedo, Vasco Meyer, Roberto BMC Res Notes Research Note OBJECTIVE: Previous works defining antigens that might be used as vaccine targets against Corynebacterium pseudotuberculosis, which is the causative agent of sheep and goat caseous lymphadenitis, have focused on secreted proteins produced in a chemically defined culture media. Considering that such antigens might not reflect the repertoire of proteins expressed during infection conditions, this experiment aimed to investigate the membrane-associated proteins with pathogenic potential expressed by C. pseudotuberculosis grown directly in animal serum. RESULTS: Its membrane-associated proteins have been extracted using an organic solvent enrichment methodology, followed by LC–MS/MS and bioinformatics analysis for protein identification and classification. The results revealed 22 membrane-associated proteins characterized as potentially pathogenic. An interaction network analysis indicated that the four potentially pathogenic proteins ciuA, fagA, OppA4 and OppCD were biologically connected within two distinct network pathways, which were both associated with the ABC Transporters KEGG pathway. These results suggest that C. pseudotuberculosis pathogenesis might be associated with the transport and uptake of nutrients; other seven identified potentially pathogenic membrane proteins also suggest that pathogenesis might involve events of bacterial resistance and adhesion. The proteins herein reported potentially reflect part of the protein repertoire expressed during real infection conditions and might be tested as vaccine antigens. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13104-018-3180-5) contains supplementary material, which is available to authorized users. BioMed Central 2018-01-25 /pmc/articles/PMC5784612/ /pubmed/29368627 http://dx.doi.org/10.1186/s13104-018-3180-5 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Note Raynal, José Tadeu Bastos, Bruno Lopes Vilas-Boas, Priscilla Carolinne Bagano Sousa, Thiago de Jesus Costa-Silva, Marcos de Sá, Maria da Conceição Aquino Portela, Ricardo Wagner Moura-Costa, Lília Ferreira Azevedo, Vasco Meyer, Roberto Identification of membrane-associated proteins with pathogenic potential expressed by Corynebacterium pseudotuberculosis grown in animal serum |
title | Identification of membrane-associated proteins with pathogenic potential expressed by Corynebacterium pseudotuberculosis grown in animal serum |
title_full | Identification of membrane-associated proteins with pathogenic potential expressed by Corynebacterium pseudotuberculosis grown in animal serum |
title_fullStr | Identification of membrane-associated proteins with pathogenic potential expressed by Corynebacterium pseudotuberculosis grown in animal serum |
title_full_unstemmed | Identification of membrane-associated proteins with pathogenic potential expressed by Corynebacterium pseudotuberculosis grown in animal serum |
title_short | Identification of membrane-associated proteins with pathogenic potential expressed by Corynebacterium pseudotuberculosis grown in animal serum |
title_sort | identification of membrane-associated proteins with pathogenic potential expressed by corynebacterium pseudotuberculosis grown in animal serum |
topic | Research Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5784612/ https://www.ncbi.nlm.nih.gov/pubmed/29368627 http://dx.doi.org/10.1186/s13104-018-3180-5 |
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