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MaRAP2‐4, a waterlogging‐responsive ERF from Mentha, regulates bidirectional sugar transporter AtSWEET10 to modulate stress response in Arabidopsis

As waterlogging and successive events severely influence growth and development of economically important plants, we attempted to characterize the role of a waterlogging‐responsive group I (A‐6) ethylene response factor (MaRAP2‐4) from Mentha arvensis. Waterlogging, ethylene and methyl jasmonate rap...

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Autores principales: Phukan, Ujjal J., Jeena, Gajendra Singh, Tripathi, Vineeta, Shukla, Rakesh Kumar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5785340/
https://www.ncbi.nlm.nih.gov/pubmed/28636266
http://dx.doi.org/10.1111/pbi.12762
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author Phukan, Ujjal J.
Jeena, Gajendra Singh
Tripathi, Vineeta
Shukla, Rakesh Kumar
author_facet Phukan, Ujjal J.
Jeena, Gajendra Singh
Tripathi, Vineeta
Shukla, Rakesh Kumar
author_sort Phukan, Ujjal J.
collection PubMed
description As waterlogging and successive events severely influence growth and development of economically important plants, we attempted to characterize the role of a waterlogging‐responsive group I (A‐6) ethylene response factor (MaRAP2‐4) from Mentha arvensis. Waterlogging, ethylene and methyl jasmonate rapidly induced the expression of MaRAP2‐4. MaRAP2‐4 interacted with multiple cis‐elements like dehydration response elements (DRE1/2), anoxia/jasmonic acid response element (JARE) and GCC box showing its involvement in multiple responses. MaRAP2‐4 localizes in the nucleus and acts as a transcriptional activator. Truncation and internal deletion identified a 20 amino acids potential transactivation domain (PLPSSVDAKLEAICQSLAIN) in MaRAP2‐4. MaRAP2‐4 transgenic Arabidopsis showed enhanced waterlogging and subsequent oxidative stress tolerance. Microarray analysis revealed that within up‐regulated genes 483, 212 and 132 promoters carry either single or multiple copies of DRE, JARE and GCC cis‐element/s, respectively. Within these promoters, a large section belongs to carbohydrate metabolism/transport, including many SWEET transporters. Further analysis showed MaRAP2‐4 specifically targets two positions in AtSWEEET10 promoter carrying DRE and/or GCC box that might regulate carbohydrate availability and waterlogging tolerance. These results demonstrate that MaRAP2‐4 is a positive regulator of waterlogging tolerance, and as energy‐consuming processes such as carbohydrate biosynthesis are reduced under waterlogging‐induced hypoxia, sugar transport through SWEETs may be the primary option to make sugar available to the required tissue.
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spelling pubmed-57853402018-02-02 MaRAP2‐4, a waterlogging‐responsive ERF from Mentha, regulates bidirectional sugar transporter AtSWEET10 to modulate stress response in Arabidopsis Phukan, Ujjal J. Jeena, Gajendra Singh Tripathi, Vineeta Shukla, Rakesh Kumar Plant Biotechnol J Research Articles As waterlogging and successive events severely influence growth and development of economically important plants, we attempted to characterize the role of a waterlogging‐responsive group I (A‐6) ethylene response factor (MaRAP2‐4) from Mentha arvensis. Waterlogging, ethylene and methyl jasmonate rapidly induced the expression of MaRAP2‐4. MaRAP2‐4 interacted with multiple cis‐elements like dehydration response elements (DRE1/2), anoxia/jasmonic acid response element (JARE) and GCC box showing its involvement in multiple responses. MaRAP2‐4 localizes in the nucleus and acts as a transcriptional activator. Truncation and internal deletion identified a 20 amino acids potential transactivation domain (PLPSSVDAKLEAICQSLAIN) in MaRAP2‐4. MaRAP2‐4 transgenic Arabidopsis showed enhanced waterlogging and subsequent oxidative stress tolerance. Microarray analysis revealed that within up‐regulated genes 483, 212 and 132 promoters carry either single or multiple copies of DRE, JARE and GCC cis‐element/s, respectively. Within these promoters, a large section belongs to carbohydrate metabolism/transport, including many SWEET transporters. Further analysis showed MaRAP2‐4 specifically targets two positions in AtSWEEET10 promoter carrying DRE and/or GCC box that might regulate carbohydrate availability and waterlogging tolerance. These results demonstrate that MaRAP2‐4 is a positive regulator of waterlogging tolerance, and as energy‐consuming processes such as carbohydrate biosynthesis are reduced under waterlogging‐induced hypoxia, sugar transport through SWEETs may be the primary option to make sugar available to the required tissue. John Wiley and Sons Inc. 2017-07-25 2018-01 /pmc/articles/PMC5785340/ /pubmed/28636266 http://dx.doi.org/10.1111/pbi.12762 Text en © 2017 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Phukan, Ujjal J.
Jeena, Gajendra Singh
Tripathi, Vineeta
Shukla, Rakesh Kumar
MaRAP2‐4, a waterlogging‐responsive ERF from Mentha, regulates bidirectional sugar transporter AtSWEET10 to modulate stress response in Arabidopsis
title MaRAP2‐4, a waterlogging‐responsive ERF from Mentha, regulates bidirectional sugar transporter AtSWEET10 to modulate stress response in Arabidopsis
title_full MaRAP2‐4, a waterlogging‐responsive ERF from Mentha, regulates bidirectional sugar transporter AtSWEET10 to modulate stress response in Arabidopsis
title_fullStr MaRAP2‐4, a waterlogging‐responsive ERF from Mentha, regulates bidirectional sugar transporter AtSWEET10 to modulate stress response in Arabidopsis
title_full_unstemmed MaRAP2‐4, a waterlogging‐responsive ERF from Mentha, regulates bidirectional sugar transporter AtSWEET10 to modulate stress response in Arabidopsis
title_short MaRAP2‐4, a waterlogging‐responsive ERF from Mentha, regulates bidirectional sugar transporter AtSWEET10 to modulate stress response in Arabidopsis
title_sort marap2‐4, a waterlogging‐responsive erf from mentha, regulates bidirectional sugar transporter atsweet10 to modulate stress response in arabidopsis
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5785340/
https://www.ncbi.nlm.nih.gov/pubmed/28636266
http://dx.doi.org/10.1111/pbi.12762
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