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Genomewide analysis of the lateral organ boundaries domain gene family in Eucalyptus grandis reveals members that differentially impact secondary growth
Lateral Organ Boundaries Domain (LBD) proteins are plant‐specific transcription factors playing crucial roles in growth and development. However, the function of LBD proteins in Eucalyptus grandis remains largely unexplored. In this study, LBD genes in E. grandis were identified and characterized us...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5785364/ https://www.ncbi.nlm.nih.gov/pubmed/28499078 http://dx.doi.org/10.1111/pbi.12754 |
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author | Lu, Qiang Shao, Fenjuan Macmillan, Colleen Wilson, Iain W. van der Merwe, Karen Hussey, Steven G. Myburg, Alexander A. Dong, Xiaomei Qiu, Deyou |
author_facet | Lu, Qiang Shao, Fenjuan Macmillan, Colleen Wilson, Iain W. van der Merwe, Karen Hussey, Steven G. Myburg, Alexander A. Dong, Xiaomei Qiu, Deyou |
author_sort | Lu, Qiang |
collection | PubMed |
description | Lateral Organ Boundaries Domain (LBD) proteins are plant‐specific transcription factors playing crucial roles in growth and development. However, the function of LBD proteins in Eucalyptus grandis remains largely unexplored. In this study, LBD genes in E. grandis were identified and characterized using bioinformatics approaches. Gene expression patterns in various tissues and the transcriptional responses of EgLBDs to exogenous hormones were determined by qRT‐PCR. Functions of the selected EgLBDs were studied by ectopically overexpressing in a hybrid poplar (Populus alba × Populus glandulosa). Expression levels of genes in the transgenic plants were investigated by RNA‐seq. Our results showed that there were forty‐six EgLBD members in the E. grandis genome and three EgLBDs displayed xylem‐ (EgLBD29) or phloem‐preferential expression (EgLBD22 and EgLBD37). Confocal microscopy indicated that EgLBD22, EgLBD 29 and EgLBD37 were localized to the nucleus. Furthermore, we found that EgLBD22, EgLBD29 and EgLBD37 were responsive to the treatments of indol‐3‐acetic acid and gibberellic acid. More importantly, we demonstrated EgLBDs exerted different influences on secondary growth. Namely, 35S::EgLBD37 led to significantly increased secondary xylem, 35S::EgLBD29 led to greatly increased phloem fibre production, and 35S::EgLBD22 showed no obvious effects. We revealed that key genes related to gibberellin, ethylene and auxin signalling pathway as well as cell expansion were significantly up‐ or down‐regulated in transgenic plants. Our new findings suggest that LBD genes in E. grandis play important roles in secondary growth. This provides new mechanisms to increase wood or fibre production. |
format | Online Article Text |
id | pubmed-5785364 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-57853642018-02-02 Genomewide analysis of the lateral organ boundaries domain gene family in Eucalyptus grandis reveals members that differentially impact secondary growth Lu, Qiang Shao, Fenjuan Macmillan, Colleen Wilson, Iain W. van der Merwe, Karen Hussey, Steven G. Myburg, Alexander A. Dong, Xiaomei Qiu, Deyou Plant Biotechnol J Research Articles Lateral Organ Boundaries Domain (LBD) proteins are plant‐specific transcription factors playing crucial roles in growth and development. However, the function of LBD proteins in Eucalyptus grandis remains largely unexplored. In this study, LBD genes in E. grandis were identified and characterized using bioinformatics approaches. Gene expression patterns in various tissues and the transcriptional responses of EgLBDs to exogenous hormones were determined by qRT‐PCR. Functions of the selected EgLBDs were studied by ectopically overexpressing in a hybrid poplar (Populus alba × Populus glandulosa). Expression levels of genes in the transgenic plants were investigated by RNA‐seq. Our results showed that there were forty‐six EgLBD members in the E. grandis genome and three EgLBDs displayed xylem‐ (EgLBD29) or phloem‐preferential expression (EgLBD22 and EgLBD37). Confocal microscopy indicated that EgLBD22, EgLBD 29 and EgLBD37 were localized to the nucleus. Furthermore, we found that EgLBD22, EgLBD29 and EgLBD37 were responsive to the treatments of indol‐3‐acetic acid and gibberellic acid. More importantly, we demonstrated EgLBDs exerted different influences on secondary growth. Namely, 35S::EgLBD37 led to significantly increased secondary xylem, 35S::EgLBD29 led to greatly increased phloem fibre production, and 35S::EgLBD22 showed no obvious effects. We revealed that key genes related to gibberellin, ethylene and auxin signalling pathway as well as cell expansion were significantly up‐ or down‐regulated in transgenic plants. Our new findings suggest that LBD genes in E. grandis play important roles in secondary growth. This provides new mechanisms to increase wood or fibre production. John Wiley and Sons Inc. 2017-07-25 2018-01 /pmc/articles/PMC5785364/ /pubmed/28499078 http://dx.doi.org/10.1111/pbi.12754 Text en © 2017 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Lu, Qiang Shao, Fenjuan Macmillan, Colleen Wilson, Iain W. van der Merwe, Karen Hussey, Steven G. Myburg, Alexander A. Dong, Xiaomei Qiu, Deyou Genomewide analysis of the lateral organ boundaries domain gene family in Eucalyptus grandis reveals members that differentially impact secondary growth |
title | Genomewide analysis of the lateral organ boundaries domain gene family in Eucalyptus grandis reveals members that differentially impact secondary growth |
title_full | Genomewide analysis of the lateral organ boundaries domain gene family in Eucalyptus grandis reveals members that differentially impact secondary growth |
title_fullStr | Genomewide analysis of the lateral organ boundaries domain gene family in Eucalyptus grandis reveals members that differentially impact secondary growth |
title_full_unstemmed | Genomewide analysis of the lateral organ boundaries domain gene family in Eucalyptus grandis reveals members that differentially impact secondary growth |
title_short | Genomewide analysis of the lateral organ boundaries domain gene family in Eucalyptus grandis reveals members that differentially impact secondary growth |
title_sort | genomewide analysis of the lateral organ boundaries domain gene family in eucalyptus grandis reveals members that differentially impact secondary growth |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5785364/ https://www.ncbi.nlm.nih.gov/pubmed/28499078 http://dx.doi.org/10.1111/pbi.12754 |
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