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Exploring traditional aus-type rice for metabolites conferring drought tolerance

BACKGROUND: Traditional varieties and landraces belonging to the aus-type group of rice (Oryza sativa L.) are known to be highly tolerant to environmental stresses, such as drought and heat, and are therefore recognized as a valuable genetic resource for crop improvement. Using two aus-type (Dular,...

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Autores principales: Casartelli, Alberto, Riewe, David, Hubberten, Hans Michael, Altmann, Thomas, Hoefgen, Rainer, Heuer, Sigrid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5785456/
https://www.ncbi.nlm.nih.gov/pubmed/29372429
http://dx.doi.org/10.1186/s12284-017-0189-7
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author Casartelli, Alberto
Riewe, David
Hubberten, Hans Michael
Altmann, Thomas
Hoefgen, Rainer
Heuer, Sigrid
author_facet Casartelli, Alberto
Riewe, David
Hubberten, Hans Michael
Altmann, Thomas
Hoefgen, Rainer
Heuer, Sigrid
author_sort Casartelli, Alberto
collection PubMed
description BACKGROUND: Traditional varieties and landraces belonging to the aus-type group of rice (Oryza sativa L.) are known to be highly tolerant to environmental stresses, such as drought and heat, and are therefore recognized as a valuable genetic resource for crop improvement. Using two aus-type (Dular, N22) and two drought intolerant irrigated varieties (IR64, IR74) an untargeted metabolomics analysis was conducted to identify drought-responsive metabolites associated with tolerance. RESULTS: The superior drought tolerance of Dular and N22 compared with the irrigated varieties was confirmed by phenotyping plants grown to maturity after imposing severe drought stress in a dry-down treatment. Dular and N22 did not show a significant reduction in grain yield compared to well-watered control plants, whereas the intolerant varieties showed a significant reduction in both, total spikelet number and grain yield. The metabolomics analysis was conducted with shoot and root samples of plants at the tillering stage at the end of the dry-down treatment. The data revealed an overall higher accumulation of N-rich metabolites (amino acids and nucleotide-related metabolites allantoin and uridine) in shoots of the tolerant varieties. In roots, the aus-type varieties were characterised by a higher reduction of metabolites representative of glycolysis and the TCA cycle, such as malate, glyceric acid and glyceric acid-3-phosphate. On the other hand, the oligosaccharide raffinose showed a higher fold increase in both, shoots and roots of the sensitive genotypes. The data further showed that, for certain drought-responsive metabolites, differences between the contrasting rice varieties were already evident under well-watered control conditions. CONCLUSIONS: The drought tolerance-related metabolites identified in the aus-type varieties provide a valuable set of protective compounds and an entry point for assessing genetic diversity in the underlying pathways for developing drought tolerant rice and other crops. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12284-017-0189-7) contains supplementary material, which is available to authorized users.
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spelling pubmed-57854562018-02-05 Exploring traditional aus-type rice for metabolites conferring drought tolerance Casartelli, Alberto Riewe, David Hubberten, Hans Michael Altmann, Thomas Hoefgen, Rainer Heuer, Sigrid Rice (N Y) Original Article BACKGROUND: Traditional varieties and landraces belonging to the aus-type group of rice (Oryza sativa L.) are known to be highly tolerant to environmental stresses, such as drought and heat, and are therefore recognized as a valuable genetic resource for crop improvement. Using two aus-type (Dular, N22) and two drought intolerant irrigated varieties (IR64, IR74) an untargeted metabolomics analysis was conducted to identify drought-responsive metabolites associated with tolerance. RESULTS: The superior drought tolerance of Dular and N22 compared with the irrigated varieties was confirmed by phenotyping plants grown to maturity after imposing severe drought stress in a dry-down treatment. Dular and N22 did not show a significant reduction in grain yield compared to well-watered control plants, whereas the intolerant varieties showed a significant reduction in both, total spikelet number and grain yield. The metabolomics analysis was conducted with shoot and root samples of plants at the tillering stage at the end of the dry-down treatment. The data revealed an overall higher accumulation of N-rich metabolites (amino acids and nucleotide-related metabolites allantoin and uridine) in shoots of the tolerant varieties. In roots, the aus-type varieties were characterised by a higher reduction of metabolites representative of glycolysis and the TCA cycle, such as malate, glyceric acid and glyceric acid-3-phosphate. On the other hand, the oligosaccharide raffinose showed a higher fold increase in both, shoots and roots of the sensitive genotypes. The data further showed that, for certain drought-responsive metabolites, differences between the contrasting rice varieties were already evident under well-watered control conditions. CONCLUSIONS: The drought tolerance-related metabolites identified in the aus-type varieties provide a valuable set of protective compounds and an entry point for assessing genetic diversity in the underlying pathways for developing drought tolerant rice and other crops. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12284-017-0189-7) contains supplementary material, which is available to authorized users. Springer US 2018-01-25 /pmc/articles/PMC5785456/ /pubmed/29372429 http://dx.doi.org/10.1186/s12284-017-0189-7 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Casartelli, Alberto
Riewe, David
Hubberten, Hans Michael
Altmann, Thomas
Hoefgen, Rainer
Heuer, Sigrid
Exploring traditional aus-type rice for metabolites conferring drought tolerance
title Exploring traditional aus-type rice for metabolites conferring drought tolerance
title_full Exploring traditional aus-type rice for metabolites conferring drought tolerance
title_fullStr Exploring traditional aus-type rice for metabolites conferring drought tolerance
title_full_unstemmed Exploring traditional aus-type rice for metabolites conferring drought tolerance
title_short Exploring traditional aus-type rice for metabolites conferring drought tolerance
title_sort exploring traditional aus-type rice for metabolites conferring drought tolerance
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5785456/
https://www.ncbi.nlm.nih.gov/pubmed/29372429
http://dx.doi.org/10.1186/s12284-017-0189-7
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