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Alterations of oral microbiota distinguish children with autism spectrum disorders from healthy controls
Altered gut microbiota is associated with autism spectrum disorders (ASD), a group of complex, fast growing but difficult-to-diagnose neurodevelopmental disorders worldwide. However, the role of the oral microbiota in ASD remains unexplored. Via high-throughput sequencing of 111 oral samples in 32 c...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5785483/ https://www.ncbi.nlm.nih.gov/pubmed/29371629 http://dx.doi.org/10.1038/s41598-018-19982-y |
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author | Qiao, Yanan Wu, Mingtao Feng, Yanhuizhi Zhou, Zhichong Chen, Lei Chen, Fengshan |
author_facet | Qiao, Yanan Wu, Mingtao Feng, Yanhuizhi Zhou, Zhichong Chen, Lei Chen, Fengshan |
author_sort | Qiao, Yanan |
collection | PubMed |
description | Altered gut microbiota is associated with autism spectrum disorders (ASD), a group of complex, fast growing but difficult-to-diagnose neurodevelopmental disorders worldwide. However, the role of the oral microbiota in ASD remains unexplored. Via high-throughput sequencing of 111 oral samples in 32 children with ASD and 27 healthy controls, we demonstrated that the salivary and dental microbiota of ASD patients were highly distinct from those of healthy individuals. Lower bacterial diversity was observed in ASD children compared to controls, especially in dental samples. Also, principal coordinate analysis revealed divergences between ASD patients and controls. Moreover, pathogens such as Haemophilus in saliva and Streptococcus in plaques showed significantly higher abundance in ASD patients, whereas commensals such as Prevotella, Selenomonas, Actinomyces, Porphyromonas, and Fusobacterium were reduced. Specifically, an overt depletion of Prevotellaceae co-occurrence network in ASD patients was obtained in dental plaques. The distinguishable bacteria were also correlated with clinical indices, reflecting disease severity and the oral health status (i.e. dental caries). Finally, diagnostic models based on key microbes were constructed, with 96.3% accuracy in saliva. Taken together, this study characterized the habitat-specific profile of the oral microbiota in ASD patients, which might help develop novel strategies for the diagnosis of ASD. |
format | Online Article Text |
id | pubmed-5785483 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-57854832018-02-07 Alterations of oral microbiota distinguish children with autism spectrum disorders from healthy controls Qiao, Yanan Wu, Mingtao Feng, Yanhuizhi Zhou, Zhichong Chen, Lei Chen, Fengshan Sci Rep Article Altered gut microbiota is associated with autism spectrum disorders (ASD), a group of complex, fast growing but difficult-to-diagnose neurodevelopmental disorders worldwide. However, the role of the oral microbiota in ASD remains unexplored. Via high-throughput sequencing of 111 oral samples in 32 children with ASD and 27 healthy controls, we demonstrated that the salivary and dental microbiota of ASD patients were highly distinct from those of healthy individuals. Lower bacterial diversity was observed in ASD children compared to controls, especially in dental samples. Also, principal coordinate analysis revealed divergences between ASD patients and controls. Moreover, pathogens such as Haemophilus in saliva and Streptococcus in plaques showed significantly higher abundance in ASD patients, whereas commensals such as Prevotella, Selenomonas, Actinomyces, Porphyromonas, and Fusobacterium were reduced. Specifically, an overt depletion of Prevotellaceae co-occurrence network in ASD patients was obtained in dental plaques. The distinguishable bacteria were also correlated with clinical indices, reflecting disease severity and the oral health status (i.e. dental caries). Finally, diagnostic models based on key microbes were constructed, with 96.3% accuracy in saliva. Taken together, this study characterized the habitat-specific profile of the oral microbiota in ASD patients, which might help develop novel strategies for the diagnosis of ASD. Nature Publishing Group UK 2018-01-25 /pmc/articles/PMC5785483/ /pubmed/29371629 http://dx.doi.org/10.1038/s41598-018-19982-y Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Qiao, Yanan Wu, Mingtao Feng, Yanhuizhi Zhou, Zhichong Chen, Lei Chen, Fengshan Alterations of oral microbiota distinguish children with autism spectrum disorders from healthy controls |
title | Alterations of oral microbiota distinguish children with autism spectrum disorders from healthy controls |
title_full | Alterations of oral microbiota distinguish children with autism spectrum disorders from healthy controls |
title_fullStr | Alterations of oral microbiota distinguish children with autism spectrum disorders from healthy controls |
title_full_unstemmed | Alterations of oral microbiota distinguish children with autism spectrum disorders from healthy controls |
title_short | Alterations of oral microbiota distinguish children with autism spectrum disorders from healthy controls |
title_sort | alterations of oral microbiota distinguish children with autism spectrum disorders from healthy controls |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5785483/ https://www.ncbi.nlm.nih.gov/pubmed/29371629 http://dx.doi.org/10.1038/s41598-018-19982-y |
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