Isolation and characterization of novel mutations in the pSC101 origin that increase copy number

pSC101 is a narrow host range, low-copy plasmid commonly used for genetically manipulating Escherichia coli. As a byproduct of a genetic screen for a more sensitive lactam biosensor, we identified multiple novel mutations that increase the copy number of plasmids with the pSC101 origin. All mutation...

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Detalles Bibliográficos
Autores principales: Thompson, Mitchell G., Sedaghatian, Nima, Barajas, Jesus F., Wehrs, Maren, Bailey, Constance B., Kaplan, Nurgul, Hillson, Nathan J., Mukhopadhyay, Aindrila, Keasling, Jay D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5785507/
https://www.ncbi.nlm.nih.gov/pubmed/29371642
http://dx.doi.org/10.1038/s41598-018-20016-w

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5785507/
https://www.ncbi.nlm.nih.gov/pubmed/29371642
http://dx.doi.org/10.1038/s41598-018-20016-w

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