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Naive-like ESRRB(+) iPSCs with the Capacity for Rapid Neural Differentiation
Several groups have reported the existence of a form of pluripotency that resembles that of mouse embryonic stem cells (mESCs), i.e., a naive state, in human pluripotent stem cells; however, the characteristics vary between reports. The nuclear receptor ESRRB is expressed in mESCs and plays a signif...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5785673/ https://www.ncbi.nlm.nih.gov/pubmed/29129686 http://dx.doi.org/10.1016/j.stemcr.2017.10.008 |
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author | Kisa, Fumihiko Shiozawa, Seiji Oda, Keisuke Yoshimatsu, Sho Nakamura, Mari Koya, Ikuko Kawai, Kenji Suzuki, Sadafumi Okano, Hideyuki |
author_facet | Kisa, Fumihiko Shiozawa, Seiji Oda, Keisuke Yoshimatsu, Sho Nakamura, Mari Koya, Ikuko Kawai, Kenji Suzuki, Sadafumi Okano, Hideyuki |
author_sort | Kisa, Fumihiko |
collection | PubMed |
description | Several groups have reported the existence of a form of pluripotency that resembles that of mouse embryonic stem cells (mESCs), i.e., a naive state, in human pluripotent stem cells; however, the characteristics vary between reports. The nuclear receptor ESRRB is expressed in mESCs and plays a significant role in their self-renewal, but its expression has not been observed in most naive-like human induced pluripotent stem cells (hiPSCs). In this study, we modified several methods for converting hiPSCs into a naive state through the transgenic expression of several reprogramming factors. The resulting cells express the components of the core transcriptional network of mESCs, including ESRRB, at high levels, which suggests the existence of naive-state hiPSCs that are similar to mESCs. We also demonstrate that these cells differentiate more readily into neural cells than do conventional hiPSCs. These features may be beneficial for their use in disease modeling and regenerative medicine. |
format | Online Article Text |
id | pubmed-5785673 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-57856732018-01-29 Naive-like ESRRB(+) iPSCs with the Capacity for Rapid Neural Differentiation Kisa, Fumihiko Shiozawa, Seiji Oda, Keisuke Yoshimatsu, Sho Nakamura, Mari Koya, Ikuko Kawai, Kenji Suzuki, Sadafumi Okano, Hideyuki Stem Cell Reports Article Several groups have reported the existence of a form of pluripotency that resembles that of mouse embryonic stem cells (mESCs), i.e., a naive state, in human pluripotent stem cells; however, the characteristics vary between reports. The nuclear receptor ESRRB is expressed in mESCs and plays a significant role in their self-renewal, but its expression has not been observed in most naive-like human induced pluripotent stem cells (hiPSCs). In this study, we modified several methods for converting hiPSCs into a naive state through the transgenic expression of several reprogramming factors. The resulting cells express the components of the core transcriptional network of mESCs, including ESRRB, at high levels, which suggests the existence of naive-state hiPSCs that are similar to mESCs. We also demonstrate that these cells differentiate more readily into neural cells than do conventional hiPSCs. These features may be beneficial for their use in disease modeling and regenerative medicine. Elsevier 2017-11-09 /pmc/articles/PMC5785673/ /pubmed/29129686 http://dx.doi.org/10.1016/j.stemcr.2017.10.008 Text en © 2017 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kisa, Fumihiko Shiozawa, Seiji Oda, Keisuke Yoshimatsu, Sho Nakamura, Mari Koya, Ikuko Kawai, Kenji Suzuki, Sadafumi Okano, Hideyuki Naive-like ESRRB(+) iPSCs with the Capacity for Rapid Neural Differentiation |
title | Naive-like ESRRB(+) iPSCs with the Capacity for Rapid Neural Differentiation |
title_full | Naive-like ESRRB(+) iPSCs with the Capacity for Rapid Neural Differentiation |
title_fullStr | Naive-like ESRRB(+) iPSCs with the Capacity for Rapid Neural Differentiation |
title_full_unstemmed | Naive-like ESRRB(+) iPSCs with the Capacity for Rapid Neural Differentiation |
title_short | Naive-like ESRRB(+) iPSCs with the Capacity for Rapid Neural Differentiation |
title_sort | naive-like esrrb(+) ipscs with the capacity for rapid neural differentiation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5785673/ https://www.ncbi.nlm.nih.gov/pubmed/29129686 http://dx.doi.org/10.1016/j.stemcr.2017.10.008 |
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