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Mode of Surgical Injury Influences the Source of Urothelial Progenitors during Bladder Defect Repair
The bladder urothelium functions as a urine-blood barrier and consists of basal, intermediate, and superficial cell populations. Reconstructive procedures such as augmentation cystoplasty and focal mucosal resection involve localized surgical damage to the bladder wall whereby focal segments of the...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5785709/ https://www.ncbi.nlm.nih.gov/pubmed/29173895 http://dx.doi.org/10.1016/j.stemcr.2017.10.025 |
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author | Schäfer, Frank-Mattias Algarrahi, Khalid Savarino, Alyssa Yang, Xuehui Seager, Catherine Franck, Debra Costa, Kyle Liu, Shanshan Logvinenko, Tanya Adam, Rosalyn Mauney, Joshua R. |
author_facet | Schäfer, Frank-Mattias Algarrahi, Khalid Savarino, Alyssa Yang, Xuehui Seager, Catherine Franck, Debra Costa, Kyle Liu, Shanshan Logvinenko, Tanya Adam, Rosalyn Mauney, Joshua R. |
author_sort | Schäfer, Frank-Mattias |
collection | PubMed |
description | The bladder urothelium functions as a urine-blood barrier and consists of basal, intermediate, and superficial cell populations. Reconstructive procedures such as augmentation cystoplasty and focal mucosal resection involve localized surgical damage to the bladder wall whereby focal segments of the urothelium and underlying submucosa are respectively removed or replaced and regeneration ensues. We demonstrate using lineage-tracing systems that urothelial regeneration following augmentation cystoplasty with acellular grafts exclusively depends on host keratin 5-expressing basal cells to repopulate all lineages of the de novo urothelium at implant sites. Conversely, repair of focal mucosal defects not only employs this mechanism, but in parallel host intermediate cell daughters expressing uroplakin 2 give rise to themselves and are also contributors to superficial cells in neotissues. These results highlight the diversity of urothelial regenerative responses to surgical injury and may lead to advancements in bladder tissue engineering approaches. |
format | Online Article Text |
id | pubmed-5785709 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-57857092018-01-29 Mode of Surgical Injury Influences the Source of Urothelial Progenitors during Bladder Defect Repair Schäfer, Frank-Mattias Algarrahi, Khalid Savarino, Alyssa Yang, Xuehui Seager, Catherine Franck, Debra Costa, Kyle Liu, Shanshan Logvinenko, Tanya Adam, Rosalyn Mauney, Joshua R. Stem Cell Reports Article The bladder urothelium functions as a urine-blood barrier and consists of basal, intermediate, and superficial cell populations. Reconstructive procedures such as augmentation cystoplasty and focal mucosal resection involve localized surgical damage to the bladder wall whereby focal segments of the urothelium and underlying submucosa are respectively removed or replaced and regeneration ensues. We demonstrate using lineage-tracing systems that urothelial regeneration following augmentation cystoplasty with acellular grafts exclusively depends on host keratin 5-expressing basal cells to repopulate all lineages of the de novo urothelium at implant sites. Conversely, repair of focal mucosal defects not only employs this mechanism, but in parallel host intermediate cell daughters expressing uroplakin 2 give rise to themselves and are also contributors to superficial cells in neotissues. These results highlight the diversity of urothelial regenerative responses to surgical injury and may lead to advancements in bladder tissue engineering approaches. Elsevier 2017-11-22 /pmc/articles/PMC5785709/ /pubmed/29173895 http://dx.doi.org/10.1016/j.stemcr.2017.10.025 Text en © 2017 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Schäfer, Frank-Mattias Algarrahi, Khalid Savarino, Alyssa Yang, Xuehui Seager, Catherine Franck, Debra Costa, Kyle Liu, Shanshan Logvinenko, Tanya Adam, Rosalyn Mauney, Joshua R. Mode of Surgical Injury Influences the Source of Urothelial Progenitors during Bladder Defect Repair |
title | Mode of Surgical Injury Influences the Source of Urothelial Progenitors during Bladder Defect Repair |
title_full | Mode of Surgical Injury Influences the Source of Urothelial Progenitors during Bladder Defect Repair |
title_fullStr | Mode of Surgical Injury Influences the Source of Urothelial Progenitors during Bladder Defect Repair |
title_full_unstemmed | Mode of Surgical Injury Influences the Source of Urothelial Progenitors during Bladder Defect Repair |
title_short | Mode of Surgical Injury Influences the Source of Urothelial Progenitors during Bladder Defect Repair |
title_sort | mode of surgical injury influences the source of urothelial progenitors during bladder defect repair |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5785709/ https://www.ncbi.nlm.nih.gov/pubmed/29173895 http://dx.doi.org/10.1016/j.stemcr.2017.10.025 |
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