Endoscopic non-ablative fractional laser therapy in an orthotopic colon tumour model

Colorectal cancer is one of the leading causes of cancer-related deaths. Although several therapeutic management strategies are available at the early colon cancer stages, such as endoscopic mucosal or submucosal dissection, associated complications often include bleeding or bowel perforations. As a...

Descripción completa

Detalles Bibliográficos
Autores principales: Yoo, Su Woong, Oh, Gyungseok, Safi, Abdul Mohaimen, Hwang, Soonjoo, Seo, Young-Seok, Lee, Kyung-Hwa, Kim, Young L., Chung, Euiheon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5785993/
https://www.ncbi.nlm.nih.gov/pubmed/29374265
http://dx.doi.org/10.1038/s41598-018-19792-2
_version_ 1783295710599315456
author Yoo, Su Woong
Oh, Gyungseok
Safi, Abdul Mohaimen
Hwang, Soonjoo
Seo, Young-Seok
Lee, Kyung-Hwa
Kim, Young L.
Chung, Euiheon
author_facet Yoo, Su Woong
Oh, Gyungseok
Safi, Abdul Mohaimen
Hwang, Soonjoo
Seo, Young-Seok
Lee, Kyung-Hwa
Kim, Young L.
Chung, Euiheon
author_sort Yoo, Su Woong
collection PubMed
description Colorectal cancer is one of the leading causes of cancer-related deaths. Although several therapeutic management strategies are available at the early colon cancer stages, such as endoscopic mucosal or submucosal dissection, associated complications often include bleeding or bowel perforations. As an alternative approach, we investigated endoscopic non-ablative fractional laser (eNAFL) irradiation as a minimally invasive therapeutic modality for the treatment of early-stage colorectal cancer. By implanting SL4-DsRed colon cancer cells into the colons of the C57BL/6 mice, we developed an orthotopic colon tumour mouse model and demonstrated the early-stage tumour growth delay following the eNAFL irradiation. Additionally, we evaluated the temperature changes in the eNAFL-irradiated area using numerical simulations, and induced inflammation using histological analysis. Our results indicate a minimal thermal damage confined to the irradiated spot, sparing the adjacent tissue and alteration in the tumour microenvironment. eNAFL irradiation may be clinically useful as a minimally invasive therapeutic intervention at the early stage of tumourigenesis. In future, an optimal eNAFL therapeutic dose should be determined, in order to increase the efficacy of this approach.
format Online
Article
Text
id pubmed-5785993
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-57859932018-02-07 Endoscopic non-ablative fractional laser therapy in an orthotopic colon tumour model Yoo, Su Woong Oh, Gyungseok Safi, Abdul Mohaimen Hwang, Soonjoo Seo, Young-Seok Lee, Kyung-Hwa Kim, Young L. Chung, Euiheon Sci Rep Article Colorectal cancer is one of the leading causes of cancer-related deaths. Although several therapeutic management strategies are available at the early colon cancer stages, such as endoscopic mucosal or submucosal dissection, associated complications often include bleeding or bowel perforations. As an alternative approach, we investigated endoscopic non-ablative fractional laser (eNAFL) irradiation as a minimally invasive therapeutic modality for the treatment of early-stage colorectal cancer. By implanting SL4-DsRed colon cancer cells into the colons of the C57BL/6 mice, we developed an orthotopic colon tumour mouse model and demonstrated the early-stage tumour growth delay following the eNAFL irradiation. Additionally, we evaluated the temperature changes in the eNAFL-irradiated area using numerical simulations, and induced inflammation using histological analysis. Our results indicate a minimal thermal damage confined to the irradiated spot, sparing the adjacent tissue and alteration in the tumour microenvironment. eNAFL irradiation may be clinically useful as a minimally invasive therapeutic intervention at the early stage of tumourigenesis. In future, an optimal eNAFL therapeutic dose should be determined, in order to increase the efficacy of this approach. Nature Publishing Group UK 2018-01-26 /pmc/articles/PMC5785993/ /pubmed/29374265 http://dx.doi.org/10.1038/s41598-018-19792-2 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Yoo, Su Woong
Oh, Gyungseok
Safi, Abdul Mohaimen
Hwang, Soonjoo
Seo, Young-Seok
Lee, Kyung-Hwa
Kim, Young L.
Chung, Euiheon
Endoscopic non-ablative fractional laser therapy in an orthotopic colon tumour model
title Endoscopic non-ablative fractional laser therapy in an orthotopic colon tumour model
title_full Endoscopic non-ablative fractional laser therapy in an orthotopic colon tumour model
title_fullStr Endoscopic non-ablative fractional laser therapy in an orthotopic colon tumour model
title_full_unstemmed Endoscopic non-ablative fractional laser therapy in an orthotopic colon tumour model
title_short Endoscopic non-ablative fractional laser therapy in an orthotopic colon tumour model
title_sort endoscopic non-ablative fractional laser therapy in an orthotopic colon tumour model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5785993/
https://www.ncbi.nlm.nih.gov/pubmed/29374265
http://dx.doi.org/10.1038/s41598-018-19792-2
work_keys_str_mv AT yoosuwoong endoscopicnonablativefractionallasertherapyinanorthotopiccolontumourmodel
AT ohgyungseok endoscopicnonablativefractionallasertherapyinanorthotopiccolontumourmodel
AT safiabdulmohaimen endoscopicnonablativefractionallasertherapyinanorthotopiccolontumourmodel
AT hwangsoonjoo endoscopicnonablativefractionallasertherapyinanorthotopiccolontumourmodel
AT seoyoungseok endoscopicnonablativefractionallasertherapyinanorthotopiccolontumourmodel
AT leekyunghwa endoscopicnonablativefractionallasertherapyinanorthotopiccolontumourmodel
AT kimyoungl endoscopicnonablativefractionallasertherapyinanorthotopiccolontumourmodel
AT chungeuiheon endoscopicnonablativefractionallasertherapyinanorthotopiccolontumourmodel