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Fingerprint analysis of Huolingshengji Formula and its neuroprotective effects in SOD1(G93A) mouse model of amyotrophic lateral sclerosis

Amyotrophic lateral sclerosis (ALS) is a fatal neurological disease characterized by progressive loss of motor neurons. There are no definitive pathogenic mechanisms and effective treatments for ALS now. Traditional Chinese medicine (TCM) plays an important role in Chinese health care system. Huolin...

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Autores principales: Zhou, Qinming, Wang, Youjie, Zhang, Jingjing, Shao, Yaping, Li, Song, Wang, Yuan, Cai, Huaibin, Feng, Yi, Le, Weidong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5786035/
https://www.ncbi.nlm.nih.gov/pubmed/29374221
http://dx.doi.org/10.1038/s41598-018-19923-9
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author Zhou, Qinming
Wang, Youjie
Zhang, Jingjing
Shao, Yaping
Li, Song
Wang, Yuan
Cai, Huaibin
Feng, Yi
Le, Weidong
author_facet Zhou, Qinming
Wang, Youjie
Zhang, Jingjing
Shao, Yaping
Li, Song
Wang, Yuan
Cai, Huaibin
Feng, Yi
Le, Weidong
author_sort Zhou, Qinming
collection PubMed
description Amyotrophic lateral sclerosis (ALS) is a fatal neurological disease characterized by progressive loss of motor neurons. There are no definitive pathogenic mechanisms and effective treatments for ALS now. Traditional Chinese medicine (TCM) plays an important role in Chinese health care system. Huolingshengji Formula (HLSJ) is a TCM formula which is applied for treating flaccid syndrome. Our previous clinical study has indicated that HLSJ may have therapeutic effects in ALS patients. In the present study, we analyzed the chemical profile of HLSJ by the high-performance liquid chromatographic (HPLC) fingerprint analysis. And we investigated the therapeutic effects and neuroprotective mechanisms of HLSJ against ALS in SOD1(G93A) mouse model. Eleven typical peaks were identified by the fingerprint analysis of HLSJ, and the HPLC method had good precision, repeatability and stability. Consistent with our clinical studies, HLSJ significantly prolonged the lifespan, extended the disease duration, and prevented the motor neuron loss in the anterior horn of the lumbar spinal cords in SOD1(G93A) ALS model mice. Additionally, HLSJ alleviated the atrophy of the gastrocnemius muscles and ameliorated the apoptotic and inflammatory levels in the spinal cords of SOD1(G93A) mice. Collectively, our study indicated that HLSJ might be a novel candidate for the treatment of ALS.
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spelling pubmed-57860352018-02-07 Fingerprint analysis of Huolingshengji Formula and its neuroprotective effects in SOD1(G93A) mouse model of amyotrophic lateral sclerosis Zhou, Qinming Wang, Youjie Zhang, Jingjing Shao, Yaping Li, Song Wang, Yuan Cai, Huaibin Feng, Yi Le, Weidong Sci Rep Article Amyotrophic lateral sclerosis (ALS) is a fatal neurological disease characterized by progressive loss of motor neurons. There are no definitive pathogenic mechanisms and effective treatments for ALS now. Traditional Chinese medicine (TCM) plays an important role in Chinese health care system. Huolingshengji Formula (HLSJ) is a TCM formula which is applied for treating flaccid syndrome. Our previous clinical study has indicated that HLSJ may have therapeutic effects in ALS patients. In the present study, we analyzed the chemical profile of HLSJ by the high-performance liquid chromatographic (HPLC) fingerprint analysis. And we investigated the therapeutic effects and neuroprotective mechanisms of HLSJ against ALS in SOD1(G93A) mouse model. Eleven typical peaks were identified by the fingerprint analysis of HLSJ, and the HPLC method had good precision, repeatability and stability. Consistent with our clinical studies, HLSJ significantly prolonged the lifespan, extended the disease duration, and prevented the motor neuron loss in the anterior horn of the lumbar spinal cords in SOD1(G93A) ALS model mice. Additionally, HLSJ alleviated the atrophy of the gastrocnemius muscles and ameliorated the apoptotic and inflammatory levels in the spinal cords of SOD1(G93A) mice. Collectively, our study indicated that HLSJ might be a novel candidate for the treatment of ALS. Nature Publishing Group UK 2018-01-26 /pmc/articles/PMC5786035/ /pubmed/29374221 http://dx.doi.org/10.1038/s41598-018-19923-9 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Zhou, Qinming
Wang, Youjie
Zhang, Jingjing
Shao, Yaping
Li, Song
Wang, Yuan
Cai, Huaibin
Feng, Yi
Le, Weidong
Fingerprint analysis of Huolingshengji Formula and its neuroprotective effects in SOD1(G93A) mouse model of amyotrophic lateral sclerosis
title Fingerprint analysis of Huolingshengji Formula and its neuroprotective effects in SOD1(G93A) mouse model of amyotrophic lateral sclerosis
title_full Fingerprint analysis of Huolingshengji Formula and its neuroprotective effects in SOD1(G93A) mouse model of amyotrophic lateral sclerosis
title_fullStr Fingerprint analysis of Huolingshengji Formula and its neuroprotective effects in SOD1(G93A) mouse model of amyotrophic lateral sclerosis
title_full_unstemmed Fingerprint analysis of Huolingshengji Formula and its neuroprotective effects in SOD1(G93A) mouse model of amyotrophic lateral sclerosis
title_short Fingerprint analysis of Huolingshengji Formula and its neuroprotective effects in SOD1(G93A) mouse model of amyotrophic lateral sclerosis
title_sort fingerprint analysis of huolingshengji formula and its neuroprotective effects in sod1(g93a) mouse model of amyotrophic lateral sclerosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5786035/
https://www.ncbi.nlm.nih.gov/pubmed/29374221
http://dx.doi.org/10.1038/s41598-018-19923-9
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