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Differential Contribution of Constituent Metal Ions to the Cytotoxic Effects of Fast-Dissolving Metal-Oxide Nanoparticles

The main mechanism of toxicity for fast-dissolving nanoparticles (NPs) is relatively simple as it originates from the intrinsic toxicity of their constituent elements rather than complicated surface reactivity. However, there is little information about the compared toxicity of fast-dissolving NP an...

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Autores principales: Jeong, Jiyoung, Kim, Sung-Hyun, Lee, Seonghan, Lee, Dong-Keon, Han, Youngju, Jeon, Soyeon, Cho, Wan-Seob
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5786562/
https://www.ncbi.nlm.nih.gov/pubmed/29403385
http://dx.doi.org/10.3389/fphar.2018.00015
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author Jeong, Jiyoung
Kim, Sung-Hyun
Lee, Seonghan
Lee, Dong-Keon
Han, Youngju
Jeon, Soyeon
Cho, Wan-Seob
author_facet Jeong, Jiyoung
Kim, Sung-Hyun
Lee, Seonghan
Lee, Dong-Keon
Han, Youngju
Jeon, Soyeon
Cho, Wan-Seob
author_sort Jeong, Jiyoung
collection PubMed
description The main mechanism of toxicity for fast-dissolving nanoparticles (NPs) is relatively simple as it originates from the intrinsic toxicity of their constituent elements rather than complicated surface reactivity. However, there is little information about the compared toxicity of fast-dissolving NP and its constituent ion, which is essential for understanding the mechanism of NP toxicity and the development of a structure-toxicity relationship (STR) model. Herein, we selected three types of fast-dissolving metal-oxide NPs (CoO, CuO, and ZnO) and constituent metal chlorides (CoCl(2), CuCl(2), and ZnCl(2)) to compare dose-response curves between NP and its constituent metal. These materials were treated relevant cell lines for inhalation setting (i.e., differentiated THP-1 cells for macrophages and A549 cells for alveolar epithelial cells) and cytotoxicity as an endpoint was evaluated at 24 h post-incubation. The results showed that CoO and CuO NPs in both cell types showed similar patterns of dose-response curves and cytotoxic potential compared to that of their respective metal chloride. On the other hand, ZnO NPs in both cell types showed a completely different dose-response curve compared to that of ZnCl(2): ZnO NPs showed modest slope and much less potential for cytotoxicity compared to that of ZnCl(2). These results imply that fast-dissolving metal-oxide NPs are not always have similar dose-response curves and toxic potentials compared to their constituent metal chlorides and this may be due to the differential mechanism of intracellular uptake of these substances and their interaction with intracellular detoxification molecules. Further investigations are needed for the use of toxic potential of metal ions as a predicting factors of fast-dissolving NPs toxicity. In addition, chelating agent specific for dissolved metal ions can be applied for the treatment of these fast-dissolving NPs.
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spelling pubmed-57865622018-02-05 Differential Contribution of Constituent Metal Ions to the Cytotoxic Effects of Fast-Dissolving Metal-Oxide Nanoparticles Jeong, Jiyoung Kim, Sung-Hyun Lee, Seonghan Lee, Dong-Keon Han, Youngju Jeon, Soyeon Cho, Wan-Seob Front Pharmacol Pharmacology The main mechanism of toxicity for fast-dissolving nanoparticles (NPs) is relatively simple as it originates from the intrinsic toxicity of their constituent elements rather than complicated surface reactivity. However, there is little information about the compared toxicity of fast-dissolving NP and its constituent ion, which is essential for understanding the mechanism of NP toxicity and the development of a structure-toxicity relationship (STR) model. Herein, we selected three types of fast-dissolving metal-oxide NPs (CoO, CuO, and ZnO) and constituent metal chlorides (CoCl(2), CuCl(2), and ZnCl(2)) to compare dose-response curves between NP and its constituent metal. These materials were treated relevant cell lines for inhalation setting (i.e., differentiated THP-1 cells for macrophages and A549 cells for alveolar epithelial cells) and cytotoxicity as an endpoint was evaluated at 24 h post-incubation. The results showed that CoO and CuO NPs in both cell types showed similar patterns of dose-response curves and cytotoxic potential compared to that of their respective metal chloride. On the other hand, ZnO NPs in both cell types showed a completely different dose-response curve compared to that of ZnCl(2): ZnO NPs showed modest slope and much less potential for cytotoxicity compared to that of ZnCl(2). These results imply that fast-dissolving metal-oxide NPs are not always have similar dose-response curves and toxic potentials compared to their constituent metal chlorides and this may be due to the differential mechanism of intracellular uptake of these substances and their interaction with intracellular detoxification molecules. Further investigations are needed for the use of toxic potential of metal ions as a predicting factors of fast-dissolving NPs toxicity. In addition, chelating agent specific for dissolved metal ions can be applied for the treatment of these fast-dissolving NPs. Frontiers Media S.A. 2018-01-22 /pmc/articles/PMC5786562/ /pubmed/29403385 http://dx.doi.org/10.3389/fphar.2018.00015 Text en Copyright © 2018 Jeong, Kim, Lee, Lee, Han, Jeon and Cho. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Jeong, Jiyoung
Kim, Sung-Hyun
Lee, Seonghan
Lee, Dong-Keon
Han, Youngju
Jeon, Soyeon
Cho, Wan-Seob
Differential Contribution of Constituent Metal Ions to the Cytotoxic Effects of Fast-Dissolving Metal-Oxide Nanoparticles
title Differential Contribution of Constituent Metal Ions to the Cytotoxic Effects of Fast-Dissolving Metal-Oxide Nanoparticles
title_full Differential Contribution of Constituent Metal Ions to the Cytotoxic Effects of Fast-Dissolving Metal-Oxide Nanoparticles
title_fullStr Differential Contribution of Constituent Metal Ions to the Cytotoxic Effects of Fast-Dissolving Metal-Oxide Nanoparticles
title_full_unstemmed Differential Contribution of Constituent Metal Ions to the Cytotoxic Effects of Fast-Dissolving Metal-Oxide Nanoparticles
title_short Differential Contribution of Constituent Metal Ions to the Cytotoxic Effects of Fast-Dissolving Metal-Oxide Nanoparticles
title_sort differential contribution of constituent metal ions to the cytotoxic effects of fast-dissolving metal-oxide nanoparticles
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5786562/
https://www.ncbi.nlm.nih.gov/pubmed/29403385
http://dx.doi.org/10.3389/fphar.2018.00015
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