Cargando…

Neural stem/progenitor cells maintained in vitro under different culture conditions alter differentiation capacity of monocytes to generate dendritic cells

Cell therapy of the nervous system disorders using neural stem/progenitor cells (NSPCs) proved its efficacy in preclinical and pilot clinical studies. The mechanisms of the beneficial effects of NSPCs transplantation include replacement of damaged cells, paracrine activation of the regeneration, and...

Descripción completa

Detalles Bibliográficos
Autores principales: Lupatov, Alexey Yu, Poltavtseva, Rimma A, Bystrykh, Oxana A, Yarygin, Konstantin N, Sukhikh, Gennady T
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Journal of Stem Cells and Regenerative Medicine 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5786647/
https://www.ncbi.nlm.nih.gov/pubmed/29391750
_version_ 1783295807980568576
author Lupatov, Alexey Yu
Poltavtseva, Rimma A
Bystrykh, Oxana A
Yarygin, Konstantin N
Sukhikh, Gennady T
author_facet Lupatov, Alexey Yu
Poltavtseva, Rimma A
Bystrykh, Oxana A
Yarygin, Konstantin N
Sukhikh, Gennady T
author_sort Lupatov, Alexey Yu
collection PubMed
description Cell therapy of the nervous system disorders using neural stem/progenitor cells (NSPCs) proved its efficacy in preclinical and pilot clinical studies. The mechanisms of the beneficial effects of NSPCs transplantation include replacement of damaged cells, paracrine activation of the regeneration, and immunomodulation. Detailed assessment of NSPCs-induced immunomodulation can contribute to better control of autoimmune reactions and inflammation in patients with neurodegenerative diseases. Interactions of NSPCs with dendritic cells (DCs), the key players in the induction of the immune system response to antigens are of particular interest. Here, we demonstrate that co-culturing of monocytes with NSPCs obtained and grown utilizing serum-containing medium instead of growth factor-containing serum-free medium, results in total suppression of monocyte differentiation into DCs. The effect is similar to the action of mesenchymal stem cells (MSCs). No significant effect on DCs maturation was observed. Cultures of NSPCs set up and maintained in serum-free medium have no influence on monocyte differentiation and DCs maturation. Therefore, the effects of NSPCs upon DC differentiation from monocytes strongly depend on culture conditions, whereas the molecular marker expression patterns are similar in both types of NSPCs cultures. In broader prospective, it means that cells with almost identical phenotypes can display opposite immunological properties depending upon culture conditions. It should be taken into account when developing NSPCs-based cell products for regenerative medicine.
format Online
Article
Text
id pubmed-5786647
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Journal of Stem Cells and Regenerative Medicine
record_format MEDLINE/PubMed
spelling pubmed-57866472018-02-01 Neural stem/progenitor cells maintained in vitro under different culture conditions alter differentiation capacity of monocytes to generate dendritic cells Lupatov, Alexey Yu Poltavtseva, Rimma A Bystrykh, Oxana A Yarygin, Konstantin N Sukhikh, Gennady T J Stem Cells Regen Med Research Article Cell therapy of the nervous system disorders using neural stem/progenitor cells (NSPCs) proved its efficacy in preclinical and pilot clinical studies. The mechanisms of the beneficial effects of NSPCs transplantation include replacement of damaged cells, paracrine activation of the regeneration, and immunomodulation. Detailed assessment of NSPCs-induced immunomodulation can contribute to better control of autoimmune reactions and inflammation in patients with neurodegenerative diseases. Interactions of NSPCs with dendritic cells (DCs), the key players in the induction of the immune system response to antigens are of particular interest. Here, we demonstrate that co-culturing of monocytes with NSPCs obtained and grown utilizing serum-containing medium instead of growth factor-containing serum-free medium, results in total suppression of monocyte differentiation into DCs. The effect is similar to the action of mesenchymal stem cells (MSCs). No significant effect on DCs maturation was observed. Cultures of NSPCs set up and maintained in serum-free medium have no influence on monocyte differentiation and DCs maturation. Therefore, the effects of NSPCs upon DC differentiation from monocytes strongly depend on culture conditions, whereas the molecular marker expression patterns are similar in both types of NSPCs cultures. In broader prospective, it means that cells with almost identical phenotypes can display opposite immunological properties depending upon culture conditions. It should be taken into account when developing NSPCs-based cell products for regenerative medicine. Journal of Stem Cells and Regenerative Medicine 2017-12-18 /pmc/articles/PMC5786647/ /pubmed/29391750 Text en Copyright © Journal of Stem Cells and Regenerative Medicine
spellingShingle Research Article
Lupatov, Alexey Yu
Poltavtseva, Rimma A
Bystrykh, Oxana A
Yarygin, Konstantin N
Sukhikh, Gennady T
Neural stem/progenitor cells maintained in vitro under different culture conditions alter differentiation capacity of monocytes to generate dendritic cells
title Neural stem/progenitor cells maintained in vitro under different culture conditions alter differentiation capacity of monocytes to generate dendritic cells
title_full Neural stem/progenitor cells maintained in vitro under different culture conditions alter differentiation capacity of monocytes to generate dendritic cells
title_fullStr Neural stem/progenitor cells maintained in vitro under different culture conditions alter differentiation capacity of monocytes to generate dendritic cells
title_full_unstemmed Neural stem/progenitor cells maintained in vitro under different culture conditions alter differentiation capacity of monocytes to generate dendritic cells
title_short Neural stem/progenitor cells maintained in vitro under different culture conditions alter differentiation capacity of monocytes to generate dendritic cells
title_sort neural stem/progenitor cells maintained in vitro under different culture conditions alter differentiation capacity of monocytes to generate dendritic cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5786647/
https://www.ncbi.nlm.nih.gov/pubmed/29391750
work_keys_str_mv AT lupatovalexeyyu neuralstemprogenitorcellsmaintainedinvitrounderdifferentcultureconditionsalterdifferentiationcapacityofmonocytestogeneratedendriticcells
AT poltavtsevarimmaa neuralstemprogenitorcellsmaintainedinvitrounderdifferentcultureconditionsalterdifferentiationcapacityofmonocytestogeneratedendriticcells
AT bystrykhoxanaa neuralstemprogenitorcellsmaintainedinvitrounderdifferentcultureconditionsalterdifferentiationcapacityofmonocytestogeneratedendriticcells
AT yaryginkonstantinn neuralstemprogenitorcellsmaintainedinvitrounderdifferentcultureconditionsalterdifferentiationcapacityofmonocytestogeneratedendriticcells
AT sukhikhgennadyt neuralstemprogenitorcellsmaintainedinvitrounderdifferentcultureconditionsalterdifferentiationcapacityofmonocytestogeneratedendriticcells