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Reduced Expression of Membrane Complement Regulatory Protein CD59 on Leukocytes following Lung Transplantation

Cellular protection against undesired effects of complement activation is provided by expression of membrane-bound complement regulatory proteins including CD59. This protein prevents membrane attack complex formation and is considered to be involved in graft accommodation. Also, CD59 downregulates...

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Autores principales: Michielsen, Laura A., Budding, Kevin, Drop, Daniël, van de Graaf, Ed A., Kardol-Hoefnagel, Tineke, Verhaar, Marianne C., van Zuilen, Arjan D., Otten, Henny G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5786830/
https://www.ncbi.nlm.nih.gov/pubmed/29403484
http://dx.doi.org/10.3389/fimmu.2017.02008
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author Michielsen, Laura A.
Budding, Kevin
Drop, Daniël
van de Graaf, Ed A.
Kardol-Hoefnagel, Tineke
Verhaar, Marianne C.
van Zuilen, Arjan D.
Otten, Henny G.
author_facet Michielsen, Laura A.
Budding, Kevin
Drop, Daniël
van de Graaf, Ed A.
Kardol-Hoefnagel, Tineke
Verhaar, Marianne C.
van Zuilen, Arjan D.
Otten, Henny G.
author_sort Michielsen, Laura A.
collection PubMed
description Cellular protection against undesired effects of complement activation is provided by expression of membrane-bound complement regulatory proteins including CD59. This protein prevents membrane attack complex formation and is considered to be involved in graft accommodation. Also, CD59 downregulates CD4+ and CD8+ T-cell activation and proliferation. It is unknown whether CD59 expression is affected by transplantation. The aim of this study was to evaluate the quantitative CD59 antigen expression on distinct leukocyte subsets following lung transplantation (n = 26) and to investigate whether this differs from pretransplantation (n = 9). The results show that CD59 expression on leukocytes is significantly lower posttransplantation compared with healthy controls (p = 0.002) and pretransplantation (p < 0.0001). Moreover, the CD59 expression diminishes posttransplantation on all distinct lymphocyte subsets (p < 0.02). This effect appeared to be specific for CD59 since the expression of other surface markers remained stable or inclined following transplantation. The highest antigen expression posttransplantation was observed on CD4+ T cells and monocytes (p ≤ 0.002). These findings show that CD59 expression on leukocytes diminishes posttransplantation, which could result in decreased resistance against complement and enhanced T-cell activation. If such reduction in CD59 expression also occurs on endothelial cells from the transplanted organ, this could lead to a change into a prothrombotic and proinflammatory phenotype.
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spelling pubmed-57868302018-02-05 Reduced Expression of Membrane Complement Regulatory Protein CD59 on Leukocytes following Lung Transplantation Michielsen, Laura A. Budding, Kevin Drop, Daniël van de Graaf, Ed A. Kardol-Hoefnagel, Tineke Verhaar, Marianne C. van Zuilen, Arjan D. Otten, Henny G. Front Immunol Immunology Cellular protection against undesired effects of complement activation is provided by expression of membrane-bound complement regulatory proteins including CD59. This protein prevents membrane attack complex formation and is considered to be involved in graft accommodation. Also, CD59 downregulates CD4+ and CD8+ T-cell activation and proliferation. It is unknown whether CD59 expression is affected by transplantation. The aim of this study was to evaluate the quantitative CD59 antigen expression on distinct leukocyte subsets following lung transplantation (n = 26) and to investigate whether this differs from pretransplantation (n = 9). The results show that CD59 expression on leukocytes is significantly lower posttransplantation compared with healthy controls (p = 0.002) and pretransplantation (p < 0.0001). Moreover, the CD59 expression diminishes posttransplantation on all distinct lymphocyte subsets (p < 0.02). This effect appeared to be specific for CD59 since the expression of other surface markers remained stable or inclined following transplantation. The highest antigen expression posttransplantation was observed on CD4+ T cells and monocytes (p ≤ 0.002). These findings show that CD59 expression on leukocytes diminishes posttransplantation, which could result in decreased resistance against complement and enhanced T-cell activation. If such reduction in CD59 expression also occurs on endothelial cells from the transplanted organ, this could lead to a change into a prothrombotic and proinflammatory phenotype. Frontiers Media S.A. 2018-01-22 /pmc/articles/PMC5786830/ /pubmed/29403484 http://dx.doi.org/10.3389/fimmu.2017.02008 Text en Copyright © 2018 Michielsen, Budding, Drop, van de Graaf, Kardol-Hoefnagel, Verhaar, van Zuilen and Otten. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Michielsen, Laura A.
Budding, Kevin
Drop, Daniël
van de Graaf, Ed A.
Kardol-Hoefnagel, Tineke
Verhaar, Marianne C.
van Zuilen, Arjan D.
Otten, Henny G.
Reduced Expression of Membrane Complement Regulatory Protein CD59 on Leukocytes following Lung Transplantation
title Reduced Expression of Membrane Complement Regulatory Protein CD59 on Leukocytes following Lung Transplantation
title_full Reduced Expression of Membrane Complement Regulatory Protein CD59 on Leukocytes following Lung Transplantation
title_fullStr Reduced Expression of Membrane Complement Regulatory Protein CD59 on Leukocytes following Lung Transplantation
title_full_unstemmed Reduced Expression of Membrane Complement Regulatory Protein CD59 on Leukocytes following Lung Transplantation
title_short Reduced Expression of Membrane Complement Regulatory Protein CD59 on Leukocytes following Lung Transplantation
title_sort reduced expression of membrane complement regulatory protein cd59 on leukocytes following lung transplantation
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5786830/
https://www.ncbi.nlm.nih.gov/pubmed/29403484
http://dx.doi.org/10.3389/fimmu.2017.02008
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