Cargando…

A cDNA Immunization Strategy to Generate Nanobodies against Membrane Proteins in Native Conformation

Nanobodies (Nbs) are soluble, versatile, single-domain binding modules derived from the VHH variable domain of heavy-chain antibodies naturally occurring in camelids. Nbs hold huge promise as novel therapeutic biologics. Membrane proteins are among the most interesting targets for therapeutic Nbs be...

Descripción completa

Detalles Bibliográficos
Autores principales:	Eden, Thomas, Menzel, Stephan, Wesolowski, Janusz, Bergmann, Philine, Nissen, Marion, Dubberke, Gudrun, Seyfried, Fabienne, Albrecht, Birte, Haag, Friedrich, Koch-Nolte, Friedrich
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2018
Materias:	Immunology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5787055/ https://www.ncbi.nlm.nih.gov/pubmed/29410663 http://dx.doi.org/10.3389/fimmu.2017.01989

Ejemplares similares

Monitoring the expression of purinoceptors and nucleotide-metabolizing ecto-enzymes with antibodies directed against proteins in native conformation
por: Möller, Sina, et al.
Publicado: (2007)

Generation and Characterization of Specific Monoclonal Antibodies and Nanobodies Directed Against the ATP-Gated Channel P2X4
por: Bergmann, Philine, et al.
Publicado: (2019)

Nanobody-Based Biologics for Modulating Purinergic Signaling in Inflammation and Immunity
por: Menzel, Stephan, et al.
Publicado: (2018)

Nanobody-Enhanced Targeting of AAV Gene Therapy Vectors
por: Eichhoff, Anna Marei, et al.
Publicado: (2019)

Nanobodies: new avenue to treat kidney disease
por: Wanner, Nicola, et al.
Publicado: (2021)

Daratumumab and Nanobody-Based Heavy Chain Antibodies Inhibit the ADPR Cyclase but not the NAD(+) Hydrolase Activity of CD38-Expressing Multiple Myeloma Cells
por: Baum, Natalie, et al.
Publicado: (2020)

Nanobodies and Nanobody-Based Human Heavy Chain Antibodies As Antitumor Therapeutics
por: Bannas, Peter, et al.
Publicado: (2017)

Targeting CD38-Expressing Multiple Myeloma and Burkitt Lymphoma Cells In Vitro with Nanobody-Based Chimeric Antigen Receptors (Nb-CARs)
por: Hambach, Julia, et al.
Publicado: (2020)

Nanobodies as modulators of inflammation: potential applications for acute brain injury
por: Rissiek, Björn, et al.
Publicado: (2014)

Nanobody-based CD38-specific heavy chain antibodies induce killing of multiple myeloma and other hematological malignancies
por: Schriewer, Levin, et al.
Publicado: (2020)

A simple, sensitive, and low‐cost FACS assay for detecting antibodies against the native SARS‐CoV‐2 spike protein
por: Hambach, Julia, et al.
Publicado: (2021)

Effective targeting of microglial P2X7 following intracerebroventricular delivery of nanobodies and nanobody-encoding AAVs
por: Pinto-Espinoza, Carolina, et al.
Publicado: (2022)

Selection of Nanobodies that Block the Enzymatic and Cytotoxic Activities of the Binary Clostridium Difficile Toxin CDT
por: Unger, Mandy, et al.
Publicado: (2015)

Targeting multiple myeloma with nanobody-based heavy chain antibodies, bispecific killer cell engagers, chimeric antigen receptors, and nanobody-displaying AAV vectors
por: Hambach, Julia, et al.
Publicado: (2022)

CD38-Specific Biparatopic Heavy Chain Antibodies Display Potent Complement-Dependent Cytotoxicity Against Multiple Myeloma Cells
por: Schütze, Kerstin, et al.
Publicado: (2018)

Validation of Nanobody and Antibody Based In Vivo Tumor Xenograft NIRF-imaging Experiments in Mice Using Ex Vivo Flow Cytometry and Microscopy
por: Bannas, Peter, et al.
Publicado: (2015)

Evaluation of P2X7 Receptor Function in Tumor Contexts Using rAAV Vector and Nanobodies (AAVnano)
por: Demeules, Mélanie, et al.
Publicado: (2020)

Current Approaches and Future Perspectives for Nanobodies in Stroke Diagnostic and Therapy
por: Jank, Larissa, et al.
Publicado: (2019)

Evaluation of nanobody-based biologics targeting purinergic checkpoints in tumor models in vivo
por: Demeules, Mélanie, et al.
Publicado: (2022)

ADP-Ribosylation Regulates the Signaling Function of IFN-γ
por: Menzel, Stephan, et al.
Publicado: (2021)

Nanobodies effectively modulate the enzymatic activity of CD38 and allow specific imaging of CD38(+) tumors in mouse models in vivo
por: Fumey, William, et al.
Publicado: (2017)

Tuning IL-2 signaling by ADP-ribosylation of CD25
por: Teege, Sophie, et al.
Publicado: (2015)

CD83 Modulates B Cell Function In Vitro: Increased IL-10 and Reduced Ig Secretion by CD83Tg B Cells
por: Kretschmer, Birte, et al.
Publicado: (2007)

ADP-ribosylation of arginine
por: Laing, Sabrina, et al.
Publicado: (2010)

P2X7 on Mouse T Cells: One Channel, Many Functions
por: Rissiek, Björn, et al.
Publicado: (2015)

Structure of cyclin G-associated kinase (GAK) trapped in different conformations using nanobodies
por: Chaikuad, Apirat, et al.
Publicado: (2014)

Half-Life Extended Nanobody-Based CD38-Specific Bispecific Killercell Engagers Induce Killing of Multiple Myeloma Cells
por: Hambach, Julia, et al.
Publicado: (2022)

Immuno-targeting the multifunctional CD38 using nanobody
por: Li, Ting, et al.
Publicado: (2016)

CD38-specific nanobodies allow in vivo imaging of multiple myeloma under daratumumab therapy
por: Pape, Luca Julius, et al.
Publicado: (2022)

Mouse CD38-Specific Heavy Chain Antibodies Inhibit CD38 GDPR-Cyclase Activity and Mediate Cytotoxicity Against Tumor Cells
por: Baum, Natalie, et al.
Publicado: (2021)

In silico characterization of the family of PARP-like poly(ADP-ribosyl)transferases (pARTs)
por: Otto, Helge, et al.
Publicado: (2005)

T Cells of Different Developmental Stages Differ in Sensitivity to Apoptosis Induced by Extracellular NAD
por: Haag, Friedrich, et al.
Publicado: (2002)

Activation of the P2X7 ion channel by soluble and covalently bound ligands
por: Schwarz, Nicole, et al.
Publicado: (2009)

A Methodological Approach Using rAAV Vectors Encoding Nanobody-Based Biologics to Evaluate ARTC2.2 and P2X7 In Vivo
por: Gondé, Henri, et al.
Publicado: (2021)

Mapping paratopes of nanobodies using native mass spectrometry and ultraviolet photodissociation
por: Macias, Luis A., et al.
Publicado: (2022)

Yeast surface display platform for rapid discovery of conformationally selective nanobodies
por: McMahon, Conor, et al.
Publicado: (2018)

Selection of nanobodies with broad neutralizing potential against primary HIV-1 strains using soluble subtype C gp140 envelope trimers
por: Koch, Kathrin, et al.
Publicado: (2017)

Ecto-ADP-ribosyltransferase ARTC2.1 functionally modulates FcγR1 and FcγR2B on murine microglia
por: Rissiek, Björn, et al.
Publicado: (2017)

Single domain antibodies: promising experimental and therapeutic tools in infection and immunity
por: Wesolowski, Janusz, et al.
Publicado: (2009)

Blocking P2X7 by intracerebroventricular injection of P2X7-specific nanobodies reduces stroke lesions
por: Wilmes, Maximilian, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_vr159srkbfgau4s4j04q344kt3