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Characterisation of Thinopyrum bessarabicum chromosomes through genome-wide introgressions into wheat

KEY MESSAGE: Genome-wide introgressions of Thinopyrum bessarabicum into wheat resulted in 12 recombinant lines. Cytological and molecular techniques allowed mapping of 1150 SNP markers across all seven chromosomes of the J genome. ABSTRACT: Thinopyrum bessarabicum (2n = 2x = 14, JJ) is an important...

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Autores principales: Grewal, Surbhi, Yang, Caiyun, Edwards, Stella Hubbart, Scholefield, Duncan, Ashling, Stephen, Burridge, Amanda J., King, Ian P., King, Julie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5787220/
https://www.ncbi.nlm.nih.gov/pubmed/29101420
http://dx.doi.org/10.1007/s00122-017-3009-y
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author Grewal, Surbhi
Yang, Caiyun
Edwards, Stella Hubbart
Scholefield, Duncan
Ashling, Stephen
Burridge, Amanda J.
King, Ian P.
King, Julie
author_facet Grewal, Surbhi
Yang, Caiyun
Edwards, Stella Hubbart
Scholefield, Duncan
Ashling, Stephen
Burridge, Amanda J.
King, Ian P.
King, Julie
author_sort Grewal, Surbhi
collection PubMed
description KEY MESSAGE: Genome-wide introgressions of Thinopyrum bessarabicum into wheat resulted in 12 recombinant lines. Cytological and molecular techniques allowed mapping of 1150 SNP markers across all seven chromosomes of the J genome. ABSTRACT: Thinopyrum bessarabicum (2n = 2x = 14, JJ) is an important source for new genetic variation for wheat improvement due to its salinity tolerance and disease resistance. Its practical utilisation in wheat improvement can be facilitated through development of genome-wide introgressions leading to a variety of different wheat–Th . bessarabicum translocation lines. In this study, we report the generation of 12 such wheat–Th . bessarabicum recombinant lines, through two different crossing strategies, which were characterized using sequential single colour and multi-colour genomic in situ hybridization (sc-GISH and mc-GISH), multi-colour fluorescent in situ hybridization (mc-FISH) and single nucleotide polymorphic (SNP) DNA markers. We also detected 13 lines containing different Th. bessarabicum chromosome aberrations through sc-GISH. Through a combination of molecular and cytological analysis of all the 25 lines containing Th. bessarabicum recombinants and chromosome aberrations we were able to physically map 1150 SNP markers onto seven Th. bessarabicum J chromosomes which were divided into 36 segmental blocks. Comparative analysis of the physical map of Th. bessarabicum and the wheat genome showed that synteny between the two species is highly conserved at the macro-level and confirmed that Th. bessarabicum contains the 4/5 translocation also present in the A genome of wheat. These wheat–Th . bessarabicum recombinant lines and SNP markers provide a useful genetic resource for wheat improvement with the latter having a wider impact as a tool for detection of introgressions from other Thinopyrum species containing the J or a closely-related genome such as Thinopyrum intermedium (J(r)J(r)J(vs)J(vs)StSt) and Thinopyrum elongatum (E(e)E(e)), respectively. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00122-017-3009-y) contains supplementary material, which is available to authorized users.
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spelling pubmed-57872202018-02-02 Characterisation of Thinopyrum bessarabicum chromosomes through genome-wide introgressions into wheat Grewal, Surbhi Yang, Caiyun Edwards, Stella Hubbart Scholefield, Duncan Ashling, Stephen Burridge, Amanda J. King, Ian P. King, Julie Theor Appl Genet Original Article KEY MESSAGE: Genome-wide introgressions of Thinopyrum bessarabicum into wheat resulted in 12 recombinant lines. Cytological and molecular techniques allowed mapping of 1150 SNP markers across all seven chromosomes of the J genome. ABSTRACT: Thinopyrum bessarabicum (2n = 2x = 14, JJ) is an important source for new genetic variation for wheat improvement due to its salinity tolerance and disease resistance. Its practical utilisation in wheat improvement can be facilitated through development of genome-wide introgressions leading to a variety of different wheat–Th . bessarabicum translocation lines. In this study, we report the generation of 12 such wheat–Th . bessarabicum recombinant lines, through two different crossing strategies, which were characterized using sequential single colour and multi-colour genomic in situ hybridization (sc-GISH and mc-GISH), multi-colour fluorescent in situ hybridization (mc-FISH) and single nucleotide polymorphic (SNP) DNA markers. We also detected 13 lines containing different Th. bessarabicum chromosome aberrations through sc-GISH. Through a combination of molecular and cytological analysis of all the 25 lines containing Th. bessarabicum recombinants and chromosome aberrations we were able to physically map 1150 SNP markers onto seven Th. bessarabicum J chromosomes which were divided into 36 segmental blocks. Comparative analysis of the physical map of Th. bessarabicum and the wheat genome showed that synteny between the two species is highly conserved at the macro-level and confirmed that Th. bessarabicum contains the 4/5 translocation also present in the A genome of wheat. These wheat–Th . bessarabicum recombinant lines and SNP markers provide a useful genetic resource for wheat improvement with the latter having a wider impact as a tool for detection of introgressions from other Thinopyrum species containing the J or a closely-related genome such as Thinopyrum intermedium (J(r)J(r)J(vs)J(vs)StSt) and Thinopyrum elongatum (E(e)E(e)), respectively. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00122-017-3009-y) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2017-11-03 2018 /pmc/articles/PMC5787220/ /pubmed/29101420 http://dx.doi.org/10.1007/s00122-017-3009-y Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Grewal, Surbhi
Yang, Caiyun
Edwards, Stella Hubbart
Scholefield, Duncan
Ashling, Stephen
Burridge, Amanda J.
King, Ian P.
King, Julie
Characterisation of Thinopyrum bessarabicum chromosomes through genome-wide introgressions into wheat
title Characterisation of Thinopyrum bessarabicum chromosomes through genome-wide introgressions into wheat
title_full Characterisation of Thinopyrum bessarabicum chromosomes through genome-wide introgressions into wheat
title_fullStr Characterisation of Thinopyrum bessarabicum chromosomes through genome-wide introgressions into wheat
title_full_unstemmed Characterisation of Thinopyrum bessarabicum chromosomes through genome-wide introgressions into wheat
title_short Characterisation of Thinopyrum bessarabicum chromosomes through genome-wide introgressions into wheat
title_sort characterisation of thinopyrum bessarabicum chromosomes through genome-wide introgressions into wheat
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5787220/
https://www.ncbi.nlm.nih.gov/pubmed/29101420
http://dx.doi.org/10.1007/s00122-017-3009-y
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