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Fine mapping of a male sterility gene ms-3 in a novel cucumber (Cucumis sativus L.) mutant

KEY MESSAGE: The cucumber male sterility gene ms - 3 was fine mapped in a 76 kb region harboring an MMD1 -like gene Csa3M006660 that may be responsible for the male sterile in cucumber. ABSTRACT: A cucumber (Cucumis sativus L.) male sterile mutant (ms-3) in an advanced-generation inbred line was ide...

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Autores principales: Han, Yike, Zhao, Fengyue, Gao, Shang, Wang, Xianyun, Wei, Aimin, Chen, Zhengwu, Liu, Nan, Tong, Xueqiang, Fu, Xinmeng, Wen, Changlong, Zhang, Zhenxian, Wang, Ningning, Du, Shengli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5787221/
https://www.ncbi.nlm.nih.gov/pubmed/29134240
http://dx.doi.org/10.1007/s00122-017-3013-2
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author Han, Yike
Zhao, Fengyue
Gao, Shang
Wang, Xianyun
Wei, Aimin
Chen, Zhengwu
Liu, Nan
Tong, Xueqiang
Fu, Xinmeng
Wen, Changlong
Zhang, Zhenxian
Wang, Ningning
Du, Shengli
author_facet Han, Yike
Zhao, Fengyue
Gao, Shang
Wang, Xianyun
Wei, Aimin
Chen, Zhengwu
Liu, Nan
Tong, Xueqiang
Fu, Xinmeng
Wen, Changlong
Zhang, Zhenxian
Wang, Ningning
Du, Shengli
author_sort Han, Yike
collection PubMed
description KEY MESSAGE: The cucumber male sterility gene ms - 3 was fine mapped in a 76 kb region harboring an MMD1 -like gene Csa3M006660 that may be responsible for the male sterile in cucumber. ABSTRACT: A cucumber (Cucumis sativus L.) male sterile mutant (ms-3) in an advanced-generation inbred line was identified, and genetic analysis revealed that the male sterility trait was controlled by a recessive nuclear gene, ms-3, which was stably inherited. Histological studies suggested that the main cause of the male sterility was defective microsporogenesis, resulting in no tetrad or microspores being formed. Bulked segregant analysis (BSA) and genotyping of an F(2) population of 2553 individuals were employed used to fine map ms-3, which was delimited to a 76 Kb region. In this region, a single non-synonymous SNP was found in the Csa3M006660 gene locus, which was predicted to result in an amino acid change. Quantitative RT-PCR analysis of Csa3M006660 was consistent with the fact that it plays a role in the early development of cucumber pollen. The protein encoded by Csa3M006660 is predicted to be homeodomain (PHD) finger protein, and the high degree of sequence conservation with homologs from a range of plant species further suggested the importance of the ms-3 non-synonymous mutation. The data presented here provide support for Csa3M006660 as the most likely candidate gene for Ms-3. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00122-017-3013-2) contains supplementary material, which is available to authorized users.
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spelling pubmed-57872212018-02-02 Fine mapping of a male sterility gene ms-3 in a novel cucumber (Cucumis sativus L.) mutant Han, Yike Zhao, Fengyue Gao, Shang Wang, Xianyun Wei, Aimin Chen, Zhengwu Liu, Nan Tong, Xueqiang Fu, Xinmeng Wen, Changlong Zhang, Zhenxian Wang, Ningning Du, Shengli Theor Appl Genet Original Article KEY MESSAGE: The cucumber male sterility gene ms - 3 was fine mapped in a 76 kb region harboring an MMD1 -like gene Csa3M006660 that may be responsible for the male sterile in cucumber. ABSTRACT: A cucumber (Cucumis sativus L.) male sterile mutant (ms-3) in an advanced-generation inbred line was identified, and genetic analysis revealed that the male sterility trait was controlled by a recessive nuclear gene, ms-3, which was stably inherited. Histological studies suggested that the main cause of the male sterility was defective microsporogenesis, resulting in no tetrad or microspores being formed. Bulked segregant analysis (BSA) and genotyping of an F(2) population of 2553 individuals were employed used to fine map ms-3, which was delimited to a 76 Kb region. In this region, a single non-synonymous SNP was found in the Csa3M006660 gene locus, which was predicted to result in an amino acid change. Quantitative RT-PCR analysis of Csa3M006660 was consistent with the fact that it plays a role in the early development of cucumber pollen. The protein encoded by Csa3M006660 is predicted to be homeodomain (PHD) finger protein, and the high degree of sequence conservation with homologs from a range of plant species further suggested the importance of the ms-3 non-synonymous mutation. The data presented here provide support for Csa3M006660 as the most likely candidate gene for Ms-3. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00122-017-3013-2) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2017-11-13 2018 /pmc/articles/PMC5787221/ /pubmed/29134240 http://dx.doi.org/10.1007/s00122-017-3013-2 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Han, Yike
Zhao, Fengyue
Gao, Shang
Wang, Xianyun
Wei, Aimin
Chen, Zhengwu
Liu, Nan
Tong, Xueqiang
Fu, Xinmeng
Wen, Changlong
Zhang, Zhenxian
Wang, Ningning
Du, Shengli
Fine mapping of a male sterility gene ms-3 in a novel cucumber (Cucumis sativus L.) mutant
title Fine mapping of a male sterility gene ms-3 in a novel cucumber (Cucumis sativus L.) mutant
title_full Fine mapping of a male sterility gene ms-3 in a novel cucumber (Cucumis sativus L.) mutant
title_fullStr Fine mapping of a male sterility gene ms-3 in a novel cucumber (Cucumis sativus L.) mutant
title_full_unstemmed Fine mapping of a male sterility gene ms-3 in a novel cucumber (Cucumis sativus L.) mutant
title_short Fine mapping of a male sterility gene ms-3 in a novel cucumber (Cucumis sativus L.) mutant
title_sort fine mapping of a male sterility gene ms-3 in a novel cucumber (cucumis sativus l.) mutant
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5787221/
https://www.ncbi.nlm.nih.gov/pubmed/29134240
http://dx.doi.org/10.1007/s00122-017-3013-2
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