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Rapid conjugative mobilization of a 100 kb segment of Bacillus subtilis chromosomal DNA is mediated by a helper plasmid with no ability for self-transfer
BACKGROUND: The conjugative plasmid, pLS20, isolated from Bacillus subtilis natto, has an outstanding capacity for rapid self-transfer. In addition, it can function as a helper plasmid, mediating the mobilization of an independently replicating co-resident plasmid. RESULTS: In this study, the oriT s...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5787278/ https://www.ncbi.nlm.nih.gov/pubmed/29374463 http://dx.doi.org/10.1186/s12934-017-0855-x |
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author | Miyano, Megumi Tanaka, Kosei Ishikawa, Shu Takenaka, Shinji Miguel-Arribas, Andrés Meijer, Wilfried J. J. Yoshida, Ken-ichi |
author_facet | Miyano, Megumi Tanaka, Kosei Ishikawa, Shu Takenaka, Shinji Miguel-Arribas, Andrés Meijer, Wilfried J. J. Yoshida, Ken-ichi |
author_sort | Miyano, Megumi |
collection | PubMed |
description | BACKGROUND: The conjugative plasmid, pLS20, isolated from Bacillus subtilis natto, has an outstanding capacity for rapid self-transfer. In addition, it can function as a helper plasmid, mediating the mobilization of an independently replicating co-resident plasmid. RESULTS: In this study, the oriT sequence of pLS20cat (oriT(LS20)) was eliminated to obtain the plasmid, pLS20catΔoriT. This resulted in the complete loss of the conjugative transfer of the plasmid but still allowed it to mobilize a co-resident mobilizable plasmid. Moreover, pLS20catΔoriT was able to mobilize longer DNA segments, up to 113 kb of chromosomal DNA containing oriT(LS20), after mixing the liquid cultures of the donor and recipient for only 15 min. CONCLUSIONS: The chromosomal DNA mobilization mediated by pLS20catΔoriT will allow us to develop a novel genetic tool for the rapid, easy, and repetitive mobilization of longer DNA segments into a recipient chromosome. |
format | Online Article Text |
id | pubmed-5787278 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-57872782018-02-08 Rapid conjugative mobilization of a 100 kb segment of Bacillus subtilis chromosomal DNA is mediated by a helper plasmid with no ability for self-transfer Miyano, Megumi Tanaka, Kosei Ishikawa, Shu Takenaka, Shinji Miguel-Arribas, Andrés Meijer, Wilfried J. J. Yoshida, Ken-ichi Microb Cell Fact Research BACKGROUND: The conjugative plasmid, pLS20, isolated from Bacillus subtilis natto, has an outstanding capacity for rapid self-transfer. In addition, it can function as a helper plasmid, mediating the mobilization of an independently replicating co-resident plasmid. RESULTS: In this study, the oriT sequence of pLS20cat (oriT(LS20)) was eliminated to obtain the plasmid, pLS20catΔoriT. This resulted in the complete loss of the conjugative transfer of the plasmid but still allowed it to mobilize a co-resident mobilizable plasmid. Moreover, pLS20catΔoriT was able to mobilize longer DNA segments, up to 113 kb of chromosomal DNA containing oriT(LS20), after mixing the liquid cultures of the donor and recipient for only 15 min. CONCLUSIONS: The chromosomal DNA mobilization mediated by pLS20catΔoriT will allow us to develop a novel genetic tool for the rapid, easy, and repetitive mobilization of longer DNA segments into a recipient chromosome. BioMed Central 2018-01-27 /pmc/articles/PMC5787278/ /pubmed/29374463 http://dx.doi.org/10.1186/s12934-017-0855-x Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Miyano, Megumi Tanaka, Kosei Ishikawa, Shu Takenaka, Shinji Miguel-Arribas, Andrés Meijer, Wilfried J. J. Yoshida, Ken-ichi Rapid conjugative mobilization of a 100 kb segment of Bacillus subtilis chromosomal DNA is mediated by a helper plasmid with no ability for self-transfer |
title | Rapid conjugative mobilization of a 100 kb segment of Bacillus subtilis chromosomal DNA is mediated by a helper plasmid with no ability for self-transfer |
title_full | Rapid conjugative mobilization of a 100 kb segment of Bacillus subtilis chromosomal DNA is mediated by a helper plasmid with no ability for self-transfer |
title_fullStr | Rapid conjugative mobilization of a 100 kb segment of Bacillus subtilis chromosomal DNA is mediated by a helper plasmid with no ability for self-transfer |
title_full_unstemmed | Rapid conjugative mobilization of a 100 kb segment of Bacillus subtilis chromosomal DNA is mediated by a helper plasmid with no ability for self-transfer |
title_short | Rapid conjugative mobilization of a 100 kb segment of Bacillus subtilis chromosomal DNA is mediated by a helper plasmid with no ability for self-transfer |
title_sort | rapid conjugative mobilization of a 100 kb segment of bacillus subtilis chromosomal dna is mediated by a helper plasmid with no ability for self-transfer |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5787278/ https://www.ncbi.nlm.nih.gov/pubmed/29374463 http://dx.doi.org/10.1186/s12934-017-0855-x |
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