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Efficient inhibition of cervical cancer by dual drugs loaded in biodegradable thermosensitive hydrogel composites
BACKGROUND AND PURPOSE: We aimed to explore the anti-tumor effect and mechanism of the combination of cisplatin (DDP)-containing thermosensitive hydrogel (PEG-PCL-PEG, or PECE) and paclitaxel (PTX)-loaded MPEG-PCL polymeric micelles called PDMP on human cervical carcinoma (HeLa) cell. In our previou...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5787464/ https://www.ncbi.nlm.nih.gov/pubmed/29416613 http://dx.doi.org/10.18632/oncotarget.22965 |
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author | Xu, Shan Du, Xiaobo Feng, Gang Zhang, Yu Li, Jie Lin, Binwei Yang, Linglin Fu, Shaozhi Wu, Jingbo |
author_facet | Xu, Shan Du, Xiaobo Feng, Gang Zhang, Yu Li, Jie Lin, Binwei Yang, Linglin Fu, Shaozhi Wu, Jingbo |
author_sort | Xu, Shan |
collection | PubMed |
description | BACKGROUND AND PURPOSE: We aimed to explore the anti-tumor effect and mechanism of the combination of cisplatin (DDP)-containing thermosensitive hydrogel (PEG-PCL-PEG, or PECE) and paclitaxel (PTX)-loaded MPEG-PCL polymeric micelles called PDMP on human cervical carcinoma (HeLa) cell. In our previous studies, we found that PDMP in situ treatment of lung cancer will be liable to have potential in Lung cancer patients. RESULTS: Compared with other treatments, PDMP was most effective in prolonging survival time (P < 0.05), inhibiting tumor growth (P < 0.05), decreasing expression of CD133 (P < 0.05), CD31 (P < 0.05), and aldehyde dehydrogenase 1 (ALDH1) (P > 0.05), inducing G1 phase arrest (P < 0.05), increasing the apoptosis rate (P < 0.05), and in expressing ATM and γ-H2AX (P < 0.05). CONCLUSIONS: PDMP is regarded as a promising anti-tumor reactant, when it comes to the treatment of cervical carcinoma. METHODS: we used a xenograft cervical cancer model to verify the anti-tumor activity of PDMP and to explore its mechanism of action. Mice were intratumorally administered with NS, PECE, PTX+DDP or PDMP. After two days of treatment, three mice per group were sacrificed and tumor tissue was harvested. Levels of histone H2AX phosphorylation (γ-H2AX) were determined by immunohistochemistry and ataxia telangiectasia mutated(ATM) protein levels were measured by western blot analysis. In addition, it would sacrifice each of group of three mice through 10 days’ treatment, what’s more, it would harvest tumor by virtue of flow cytometry and immunohistochemical analysis. It would like to use there maining mice to analyze tumor growth and survival. The remaining mice were analyzed for tumor growth and survival. |
format | Online Article Text |
id | pubmed-5787464 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-57874642018-02-07 Efficient inhibition of cervical cancer by dual drugs loaded in biodegradable thermosensitive hydrogel composites Xu, Shan Du, Xiaobo Feng, Gang Zhang, Yu Li, Jie Lin, Binwei Yang, Linglin Fu, Shaozhi Wu, Jingbo Oncotarget Research Paper BACKGROUND AND PURPOSE: We aimed to explore the anti-tumor effect and mechanism of the combination of cisplatin (DDP)-containing thermosensitive hydrogel (PEG-PCL-PEG, or PECE) and paclitaxel (PTX)-loaded MPEG-PCL polymeric micelles called PDMP on human cervical carcinoma (HeLa) cell. In our previous studies, we found that PDMP in situ treatment of lung cancer will be liable to have potential in Lung cancer patients. RESULTS: Compared with other treatments, PDMP was most effective in prolonging survival time (P < 0.05), inhibiting tumor growth (P < 0.05), decreasing expression of CD133 (P < 0.05), CD31 (P < 0.05), and aldehyde dehydrogenase 1 (ALDH1) (P > 0.05), inducing G1 phase arrest (P < 0.05), increasing the apoptosis rate (P < 0.05), and in expressing ATM and γ-H2AX (P < 0.05). CONCLUSIONS: PDMP is regarded as a promising anti-tumor reactant, when it comes to the treatment of cervical carcinoma. METHODS: we used a xenograft cervical cancer model to verify the anti-tumor activity of PDMP and to explore its mechanism of action. Mice were intratumorally administered with NS, PECE, PTX+DDP or PDMP. After two days of treatment, three mice per group were sacrificed and tumor tissue was harvested. Levels of histone H2AX phosphorylation (γ-H2AX) were determined by immunohistochemistry and ataxia telangiectasia mutated(ATM) protein levels were measured by western blot analysis. In addition, it would sacrifice each of group of three mice through 10 days’ treatment, what’s more, it would harvest tumor by virtue of flow cytometry and immunohistochemical analysis. It would like to use there maining mice to analyze tumor growth and survival. The remaining mice were analyzed for tumor growth and survival. Impact Journals LLC 2017-12-06 /pmc/articles/PMC5787464/ /pubmed/29416613 http://dx.doi.org/10.18632/oncotarget.22965 Text en Copyright: © 2018 Xu et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Xu, Shan Du, Xiaobo Feng, Gang Zhang, Yu Li, Jie Lin, Binwei Yang, Linglin Fu, Shaozhi Wu, Jingbo Efficient inhibition of cervical cancer by dual drugs loaded in biodegradable thermosensitive hydrogel composites |
title | Efficient inhibition of cervical cancer by dual drugs loaded in biodegradable thermosensitive hydrogel composites |
title_full | Efficient inhibition of cervical cancer by dual drugs loaded in biodegradable thermosensitive hydrogel composites |
title_fullStr | Efficient inhibition of cervical cancer by dual drugs loaded in biodegradable thermosensitive hydrogel composites |
title_full_unstemmed | Efficient inhibition of cervical cancer by dual drugs loaded in biodegradable thermosensitive hydrogel composites |
title_short | Efficient inhibition of cervical cancer by dual drugs loaded in biodegradable thermosensitive hydrogel composites |
title_sort | efficient inhibition of cervical cancer by dual drugs loaded in biodegradable thermosensitive hydrogel composites |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5787464/ https://www.ncbi.nlm.nih.gov/pubmed/29416613 http://dx.doi.org/10.18632/oncotarget.22965 |
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