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Collective Sensing of β-Cells Generates the Metabolic Code

Major part of a pancreatic islet is composed of β-cells that secrete insulin, a key hormone regulating influx of nutrients into all cells in a vertebrate organism to support nutrition, housekeeping or energy storage. β-cells constantly communicate with each other using both direct, short-range inter...

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Autores principales: Korošak, Dean, Slak Rupnik, Marjan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5787558/
https://www.ncbi.nlm.nih.gov/pubmed/29416515
http://dx.doi.org/10.3389/fphys.2018.00031
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author Korošak, Dean
Slak Rupnik, Marjan
author_facet Korošak, Dean
Slak Rupnik, Marjan
author_sort Korošak, Dean
collection PubMed
description Major part of a pancreatic islet is composed of β-cells that secrete insulin, a key hormone regulating influx of nutrients into all cells in a vertebrate organism to support nutrition, housekeeping or energy storage. β-cells constantly communicate with each other using both direct, short-range interactions through gap junctions, and paracrine long-range signaling. However, how these cell interactions shape collective sensing and cell behavior in islets that leads to insulin release is unknown. When stimulated by specific ligands, primarily glucose, β-cells collectively respond with expression of a series of transient Ca(2+) changes on several temporal scales. Here we reanalyze a set of Ca(2+) spike trains recorded in acute rodent pancreatic tissue slice under physiological conditions. We found strongly correlated states of co-spiking cells coexisting with mostly weak pairwise correlations widespread across the islet. Furthermore, the collective Ca(2+) spiking activity in islet shows on-off intermittency with scaling of spiking amplitudes, and stimulus dependent autoassociative memory features. We use a simple spin glass-like model for the functional network of a β-cell collective to describe these findings and argue that Ca(2+) spike trains produced by collective sensing of β-cells constitute part of the islet metabolic code that regulates insulin release and limits the islet size.
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spelling pubmed-57875582018-02-07 Collective Sensing of β-Cells Generates the Metabolic Code Korošak, Dean Slak Rupnik, Marjan Front Physiol Physiology Major part of a pancreatic islet is composed of β-cells that secrete insulin, a key hormone regulating influx of nutrients into all cells in a vertebrate organism to support nutrition, housekeeping or energy storage. β-cells constantly communicate with each other using both direct, short-range interactions through gap junctions, and paracrine long-range signaling. However, how these cell interactions shape collective sensing and cell behavior in islets that leads to insulin release is unknown. When stimulated by specific ligands, primarily glucose, β-cells collectively respond with expression of a series of transient Ca(2+) changes on several temporal scales. Here we reanalyze a set of Ca(2+) spike trains recorded in acute rodent pancreatic tissue slice under physiological conditions. We found strongly correlated states of co-spiking cells coexisting with mostly weak pairwise correlations widespread across the islet. Furthermore, the collective Ca(2+) spiking activity in islet shows on-off intermittency with scaling of spiking amplitudes, and stimulus dependent autoassociative memory features. We use a simple spin glass-like model for the functional network of a β-cell collective to describe these findings and argue that Ca(2+) spike trains produced by collective sensing of β-cells constitute part of the islet metabolic code that regulates insulin release and limits the islet size. Frontiers Media S.A. 2018-01-24 /pmc/articles/PMC5787558/ /pubmed/29416515 http://dx.doi.org/10.3389/fphys.2018.00031 Text en Copyright © 2018 Korošak and Slak Rupnik. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Korošak, Dean
Slak Rupnik, Marjan
Collective Sensing of β-Cells Generates the Metabolic Code
title Collective Sensing of β-Cells Generates the Metabolic Code
title_full Collective Sensing of β-Cells Generates the Metabolic Code
title_fullStr Collective Sensing of β-Cells Generates the Metabolic Code
title_full_unstemmed Collective Sensing of β-Cells Generates the Metabolic Code
title_short Collective Sensing of β-Cells Generates the Metabolic Code
title_sort collective sensing of β-cells generates the metabolic code
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5787558/
https://www.ncbi.nlm.nih.gov/pubmed/29416515
http://dx.doi.org/10.3389/fphys.2018.00031
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