Cargando…
Induction of Short NFATc1/αA Isoform Interferes with Peripheral B Cell Differentiation
In lymphocytes, immune receptor signals induce the rapid nuclear translocation of preformed cytosolic NFAT proteins. Along with co-stimulatory signals, persistent immune receptor signals lead to high levels of NFATc1/αA, a short NFATc1 isoform, in effector lymphocytes. Whereas NFATc1 is not expresse...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5787671/ https://www.ncbi.nlm.nih.gov/pubmed/29416540 http://dx.doi.org/10.3389/fimmu.2018.00032 |
_version_ | 1783295979558010880 |
---|---|
author | Muhammad, Khalid Rudolf, Ronald Pham, Duong Anh Thuy Klein-Hessling, Stefan Takata, Katsuyoshi Matsushita, Nobuko Ellenrieder, Volker Kondo, Eisaku Serfling, Edgar |
author_facet | Muhammad, Khalid Rudolf, Ronald Pham, Duong Anh Thuy Klein-Hessling, Stefan Takata, Katsuyoshi Matsushita, Nobuko Ellenrieder, Volker Kondo, Eisaku Serfling, Edgar |
author_sort | Muhammad, Khalid |
collection | PubMed |
description | In lymphocytes, immune receptor signals induce the rapid nuclear translocation of preformed cytosolic NFAT proteins. Along with co-stimulatory signals, persistent immune receptor signals lead to high levels of NFATc1/αA, a short NFATc1 isoform, in effector lymphocytes. Whereas NFATc1 is not expressed in plasma cells, in germinal centers numerous centrocytic B cells express nuclear NFATc1/αA. When overexpressed in chicken DT40 B cells or murine WEHI 231 B cells, NFATc1/αA suppressed their cell death induced by B cell receptor signals and affected the expression of genes controlling the germinal center reaction and plasma cell formation. Among those is the Prdm1 gene encoding Blimp-1, a key factor of plasma cell formation. By binding to a regulatory DNA element within exon 1 of the Prdm1 gene, NFATc1/αA suppresses Blimp-1 expression. Since expression of a constitutive active version of NFATc1/αA interfered with Prdm1 RNA expression, LPS-mediated differentiation of splenic B cells to plasmablasts in vitro and reduced immunoglobulin production in vivo, one may conclude that NFATc1/αA plays an important role in controlling plasmablast/plasma cell formation. |
format | Online Article Text |
id | pubmed-5787671 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-57876712018-02-07 Induction of Short NFATc1/αA Isoform Interferes with Peripheral B Cell Differentiation Muhammad, Khalid Rudolf, Ronald Pham, Duong Anh Thuy Klein-Hessling, Stefan Takata, Katsuyoshi Matsushita, Nobuko Ellenrieder, Volker Kondo, Eisaku Serfling, Edgar Front Immunol Immunology In lymphocytes, immune receptor signals induce the rapid nuclear translocation of preformed cytosolic NFAT proteins. Along with co-stimulatory signals, persistent immune receptor signals lead to high levels of NFATc1/αA, a short NFATc1 isoform, in effector lymphocytes. Whereas NFATc1 is not expressed in plasma cells, in germinal centers numerous centrocytic B cells express nuclear NFATc1/αA. When overexpressed in chicken DT40 B cells or murine WEHI 231 B cells, NFATc1/αA suppressed their cell death induced by B cell receptor signals and affected the expression of genes controlling the germinal center reaction and plasma cell formation. Among those is the Prdm1 gene encoding Blimp-1, a key factor of plasma cell formation. By binding to a regulatory DNA element within exon 1 of the Prdm1 gene, NFATc1/αA suppresses Blimp-1 expression. Since expression of a constitutive active version of NFATc1/αA interfered with Prdm1 RNA expression, LPS-mediated differentiation of splenic B cells to plasmablasts in vitro and reduced immunoglobulin production in vivo, one may conclude that NFATc1/αA plays an important role in controlling plasmablast/plasma cell formation. Frontiers Media S.A. 2018-01-24 /pmc/articles/PMC5787671/ /pubmed/29416540 http://dx.doi.org/10.3389/fimmu.2018.00032 Text en Copyright © 2018 Muhammad, Rudolf, Pham, Klein-Hessling, Takata, Matsushita, Ellenrieder, Kondo and Serfling. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Muhammad, Khalid Rudolf, Ronald Pham, Duong Anh Thuy Klein-Hessling, Stefan Takata, Katsuyoshi Matsushita, Nobuko Ellenrieder, Volker Kondo, Eisaku Serfling, Edgar Induction of Short NFATc1/αA Isoform Interferes with Peripheral B Cell Differentiation |
title | Induction of Short NFATc1/αA Isoform Interferes with Peripheral B Cell Differentiation |
title_full | Induction of Short NFATc1/αA Isoform Interferes with Peripheral B Cell Differentiation |
title_fullStr | Induction of Short NFATc1/αA Isoform Interferes with Peripheral B Cell Differentiation |
title_full_unstemmed | Induction of Short NFATc1/αA Isoform Interferes with Peripheral B Cell Differentiation |
title_short | Induction of Short NFATc1/αA Isoform Interferes with Peripheral B Cell Differentiation |
title_sort | induction of short nfatc1/αa isoform interferes with peripheral b cell differentiation |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5787671/ https://www.ncbi.nlm.nih.gov/pubmed/29416540 http://dx.doi.org/10.3389/fimmu.2018.00032 |
work_keys_str_mv | AT muhammadkhalid inductionofshortnfatc1aaisoforminterfereswithperipheralbcelldifferentiation AT rudolfronald inductionofshortnfatc1aaisoforminterfereswithperipheralbcelldifferentiation AT phamduonganhthuy inductionofshortnfatc1aaisoforminterfereswithperipheralbcelldifferentiation AT kleinhesslingstefan inductionofshortnfatc1aaisoforminterfereswithperipheralbcelldifferentiation AT takatakatsuyoshi inductionofshortnfatc1aaisoforminterfereswithperipheralbcelldifferentiation AT matsushitanobuko inductionofshortnfatc1aaisoforminterfereswithperipheralbcelldifferentiation AT ellenriedervolker inductionofshortnfatc1aaisoforminterfereswithperipheralbcelldifferentiation AT kondoeisaku inductionofshortnfatc1aaisoforminterfereswithperipheralbcelldifferentiation AT serflingedgar inductionofshortnfatc1aaisoforminterfereswithperipheralbcelldifferentiation |