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Communication between N terminus and loop2 tunes Orai activation

Ca(2+) release-activated Ca(2+) (CRAC) channels constitute the major Ca(2+) entry pathway into the cell. They are fully reconstituted via intermembrane coupling of the Ca(2+)-selective Orai channel and the Ca(2+)-sensing protein STIM1. In addition to the Orai C terminus, the main coupling site for S...

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Autores principales: Fahrner, Marc, Pandey, Saurabh K., Muik, Martin, Traxler, Lukas, Butorac, Carmen, Stadlbauer, Michael, Zayats, Vasilina, Krizova, Adéla, Plenk, Peter, Frischauf, Irene, Schindl, Rainer, Gruber, Hermann J., Hinterdorfer, Peter, Ettrich, Rüdiger, Romanin, Christoph, Derler, Isabella
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5787804/
https://www.ncbi.nlm.nih.gov/pubmed/29237733
http://dx.doi.org/10.1074/jbc.M117.812693
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author Fahrner, Marc
Pandey, Saurabh K.
Muik, Martin
Traxler, Lukas
Butorac, Carmen
Stadlbauer, Michael
Zayats, Vasilina
Krizova, Adéla
Plenk, Peter
Frischauf, Irene
Schindl, Rainer
Gruber, Hermann J.
Hinterdorfer, Peter
Ettrich, Rüdiger
Romanin, Christoph
Derler, Isabella
author_facet Fahrner, Marc
Pandey, Saurabh K.
Muik, Martin
Traxler, Lukas
Butorac, Carmen
Stadlbauer, Michael
Zayats, Vasilina
Krizova, Adéla
Plenk, Peter
Frischauf, Irene
Schindl, Rainer
Gruber, Hermann J.
Hinterdorfer, Peter
Ettrich, Rüdiger
Romanin, Christoph
Derler, Isabella
author_sort Fahrner, Marc
collection PubMed
description Ca(2+) release-activated Ca(2+) (CRAC) channels constitute the major Ca(2+) entry pathway into the cell. They are fully reconstituted via intermembrane coupling of the Ca(2+)-selective Orai channel and the Ca(2+)-sensing protein STIM1. In addition to the Orai C terminus, the main coupling site for STIM1, the Orai N terminus is indispensable for Orai channel gating. Although the extended transmembrane Orai N-terminal region (Orai1 amino acids 73–91; Orai3 amino acids 48–65) is fully conserved in the Orai1 and Orai3 isoforms, Orai3 tolerates larger N-terminal truncations than Orai1 in retaining store-operated activation. In an attempt to uncover the reason for these isoform-specific structural requirements, we analyzed a series of Orai mutants and chimeras. We discovered that it was not the N termini, but the loop2 regions connecting TM2 and TM3 of Orai1 and Orai3 that featured distinct properties, which explained the different, isoform-specific behavior of Orai N-truncation mutants. Atomic force microscopy studies and MD simulations suggested that the remaining N-terminal portion in the non-functional Orai1 N-truncation mutants formed new, inhibitory interactions with the Orai1-loop2 regions, but not with Orai3-loop2. Such a loop2 swap restored activation of the N-truncation Orai1 mutants. To mimic interactions between the N terminus and loop2 in full-length Orai1 channels, we induced close proximity of the N terminus and loop2 via cysteine cross-linking, which actually caused significant inhibition of STIM1-mediated Orai currents. In aggregate, maintenance of Orai activation required not only the conserved N-terminal region but also permissive communication of the Orai N terminus and loop2 in an isoform-specific manner.
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spelling pubmed-57878042018-01-31 Communication between N terminus and loop2 tunes Orai activation Fahrner, Marc Pandey, Saurabh K. Muik, Martin Traxler, Lukas Butorac, Carmen Stadlbauer, Michael Zayats, Vasilina Krizova, Adéla Plenk, Peter Frischauf, Irene Schindl, Rainer Gruber, Hermann J. Hinterdorfer, Peter Ettrich, Rüdiger Romanin, Christoph Derler, Isabella J Biol Chem Signal Transduction Ca(2+) release-activated Ca(2+) (CRAC) channels constitute the major Ca(2+) entry pathway into the cell. They are fully reconstituted via intermembrane coupling of the Ca(2+)-selective Orai channel and the Ca(2+)-sensing protein STIM1. In addition to the Orai C terminus, the main coupling site for STIM1, the Orai N terminus is indispensable for Orai channel gating. Although the extended transmembrane Orai N-terminal region (Orai1 amino acids 73–91; Orai3 amino acids 48–65) is fully conserved in the Orai1 and Orai3 isoforms, Orai3 tolerates larger N-terminal truncations than Orai1 in retaining store-operated activation. In an attempt to uncover the reason for these isoform-specific structural requirements, we analyzed a series of Orai mutants and chimeras. We discovered that it was not the N termini, but the loop2 regions connecting TM2 and TM3 of Orai1 and Orai3 that featured distinct properties, which explained the different, isoform-specific behavior of Orai N-truncation mutants. Atomic force microscopy studies and MD simulations suggested that the remaining N-terminal portion in the non-functional Orai1 N-truncation mutants formed new, inhibitory interactions with the Orai1-loop2 regions, but not with Orai3-loop2. Such a loop2 swap restored activation of the N-truncation Orai1 mutants. To mimic interactions between the N terminus and loop2 in full-length Orai1 channels, we induced close proximity of the N terminus and loop2 via cysteine cross-linking, which actually caused significant inhibition of STIM1-mediated Orai currents. In aggregate, maintenance of Orai activation required not only the conserved N-terminal region but also permissive communication of the Orai N terminus and loop2 in an isoform-specific manner. American Society for Biochemistry and Molecular Biology 2018-01-26 2017-12-13 /pmc/articles/PMC5787804/ /pubmed/29237733 http://dx.doi.org/10.1074/jbc.M117.812693 Text en © 2018 by The American Society for Biochemistry and Molecular Biology, Inc. Author's Choice—Final version free via Creative Commons CC-BY license (http://creativecommons.org/licenses/by/4.0) .
spellingShingle Signal Transduction
Fahrner, Marc
Pandey, Saurabh K.
Muik, Martin
Traxler, Lukas
Butorac, Carmen
Stadlbauer, Michael
Zayats, Vasilina
Krizova, Adéla
Plenk, Peter
Frischauf, Irene
Schindl, Rainer
Gruber, Hermann J.
Hinterdorfer, Peter
Ettrich, Rüdiger
Romanin, Christoph
Derler, Isabella
Communication between N terminus and loop2 tunes Orai activation
title Communication between N terminus and loop2 tunes Orai activation
title_full Communication between N terminus and loop2 tunes Orai activation
title_fullStr Communication between N terminus and loop2 tunes Orai activation
title_full_unstemmed Communication between N terminus and loop2 tunes Orai activation
title_short Communication between N terminus and loop2 tunes Orai activation
title_sort communication between n terminus and loop2 tunes orai activation
topic Signal Transduction
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5787804/
https://www.ncbi.nlm.nih.gov/pubmed/29237733
http://dx.doi.org/10.1074/jbc.M117.812693
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