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Relationship of antioxidant system and reactive oxygen species with clinical semen parameters in infertile men
AIMS AND OBJECTIVES: To determine the correlation of antioxidant system and reactive oxygen species with clinical parameters in infertile semen samples. MATERIALS AND METHODS: Semen sample of fifty infertile men were divided into three groups: (1) Group I - Normospermic (count >15 million/ml), Gr...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Medknow Publications & Media Pvt Ltd
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5787958/ https://www.ncbi.nlm.nih.gov/pubmed/29417011 http://dx.doi.org/10.4103/2249-4863.222051 |
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author | Tomar, Gajendra Joshi, Tulika Varghes, Alex Sasidharan, Suresh Kural, Moolraj R. |
author_facet | Tomar, Gajendra Joshi, Tulika Varghes, Alex Sasidharan, Suresh Kural, Moolraj R. |
author_sort | Tomar, Gajendra |
collection | PubMed |
description | AIMS AND OBJECTIVES: To determine the correlation of antioxidant system and reactive oxygen species with clinical parameters in infertile semen samples. MATERIALS AND METHODS: Semen sample of fifty infertile men were divided into three groups: (1) Group I - Normospermic (count >15 million/ml), Group II - Asthenospermic (motility <32%), and Group III - Oligospermic (counts <15 million/ml) subjects based on the sperm count and sperm motility. The samples were also divided into two groups: (1) Group IV with semen pH >7.2 (25 samples) and Group V - Semen pH <7.2 (25 samples). The grouping was based on the WHO guideline for semen analysis (12). The semen antioxidant parameters like glucose-6-phosphate dehydrogenase (G-6-PDH) (spectrophotometric method Kornberg and Horecker, 1955). Catalase (Maehly and Chance 1954), glutathione peroxidase (GPX) (Rotruck method), glutathione (GSH) (dithiobisnitro-benzoate method), superoxide dismutase (SOD) (direct method), and malondialdehyde (MDA) (thiobarbituric acid reactive substances assay kit method) were investigated. Mann–Whitney U-test was applied to compare the findings. RESULTS: Of fifty semen samples there were 12 normospermic (sperm concentration ≥15 × 10(6)/ml of ejaculates), 24 asthenospermic (sperm motility ≤32%), and 14 oligospermic (sperm concentration ≤15 × 10(6)/ml of ejaculates) subjects. Results suggested that all asthenospermic males were found to have reduced motility and viability when compared with normospermic and oligospermic subjects. Activity of antioxidant parameters such as G-6-PDH, GPX, GSH, and SOD was decreased in case of asthenospermic subjects. The concentration of MDA was increased significantly (P < 0.001) in semen of asthenospermic subjects compared to normospermic and oligospermic subjects. CONCLUSION: The current study concludes that there is a significant relationship of ROS and semen parameters. Further studies will be needed in such subjects regarding role of effectiveness of dietary antioxidants in improving semen qualities. |
format | Online Article Text |
id | pubmed-5787958 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-57879582018-02-07 Relationship of antioxidant system and reactive oxygen species with clinical semen parameters in infertile men Tomar, Gajendra Joshi, Tulika Varghes, Alex Sasidharan, Suresh Kural, Moolraj R. J Family Med Prim Care Original Article AIMS AND OBJECTIVES: To determine the correlation of antioxidant system and reactive oxygen species with clinical parameters in infertile semen samples. MATERIALS AND METHODS: Semen sample of fifty infertile men were divided into three groups: (1) Group I - Normospermic (count >15 million/ml), Group II - Asthenospermic (motility <32%), and Group III - Oligospermic (counts <15 million/ml) subjects based on the sperm count and sperm motility. The samples were also divided into two groups: (1) Group IV with semen pH >7.2 (25 samples) and Group V - Semen pH <7.2 (25 samples). The grouping was based on the WHO guideline for semen analysis (12). The semen antioxidant parameters like glucose-6-phosphate dehydrogenase (G-6-PDH) (spectrophotometric method Kornberg and Horecker, 1955). Catalase (Maehly and Chance 1954), glutathione peroxidase (GPX) (Rotruck method), glutathione (GSH) (dithiobisnitro-benzoate method), superoxide dismutase (SOD) (direct method), and malondialdehyde (MDA) (thiobarbituric acid reactive substances assay kit method) were investigated. Mann–Whitney U-test was applied to compare the findings. RESULTS: Of fifty semen samples there were 12 normospermic (sperm concentration ≥15 × 10(6)/ml of ejaculates), 24 asthenospermic (sperm motility ≤32%), and 14 oligospermic (sperm concentration ≤15 × 10(6)/ml of ejaculates) subjects. Results suggested that all asthenospermic males were found to have reduced motility and viability when compared with normospermic and oligospermic subjects. Activity of antioxidant parameters such as G-6-PDH, GPX, GSH, and SOD was decreased in case of asthenospermic subjects. The concentration of MDA was increased significantly (P < 0.001) in semen of asthenospermic subjects compared to normospermic and oligospermic subjects. CONCLUSION: The current study concludes that there is a significant relationship of ROS and semen parameters. Further studies will be needed in such subjects regarding role of effectiveness of dietary antioxidants in improving semen qualities. Medknow Publications & Media Pvt Ltd 2017 /pmc/articles/PMC5787958/ /pubmed/29417011 http://dx.doi.org/10.4103/2249-4863.222051 Text en Copyright: © 2017 Journal of Family Medicine and Primary Care http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Tomar, Gajendra Joshi, Tulika Varghes, Alex Sasidharan, Suresh Kural, Moolraj R. Relationship of antioxidant system and reactive oxygen species with clinical semen parameters in infertile men |
title | Relationship of antioxidant system and reactive oxygen species with clinical semen parameters in infertile men |
title_full | Relationship of antioxidant system and reactive oxygen species with clinical semen parameters in infertile men |
title_fullStr | Relationship of antioxidant system and reactive oxygen species with clinical semen parameters in infertile men |
title_full_unstemmed | Relationship of antioxidant system and reactive oxygen species with clinical semen parameters in infertile men |
title_short | Relationship of antioxidant system and reactive oxygen species with clinical semen parameters in infertile men |
title_sort | relationship of antioxidant system and reactive oxygen species with clinical semen parameters in infertile men |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5787958/ https://www.ncbi.nlm.nih.gov/pubmed/29417011 http://dx.doi.org/10.4103/2249-4863.222051 |
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