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Relationship of antioxidant system and reactive oxygen species with clinical semen parameters in infertile men

AIMS AND OBJECTIVES: To determine the correlation of antioxidant system and reactive oxygen species with clinical parameters in infertile semen samples. MATERIALS AND METHODS: Semen sample of fifty infertile men were divided into three groups: (1) Group I - Normospermic (count >15 million/ml), Gr...

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Autores principales: Tomar, Gajendra, Joshi, Tulika, Varghes, Alex, Sasidharan, Suresh, Kural, Moolraj R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5787958/
https://www.ncbi.nlm.nih.gov/pubmed/29417011
http://dx.doi.org/10.4103/2249-4863.222051
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author Tomar, Gajendra
Joshi, Tulika
Varghes, Alex
Sasidharan, Suresh
Kural, Moolraj R.
author_facet Tomar, Gajendra
Joshi, Tulika
Varghes, Alex
Sasidharan, Suresh
Kural, Moolraj R.
author_sort Tomar, Gajendra
collection PubMed
description AIMS AND OBJECTIVES: To determine the correlation of antioxidant system and reactive oxygen species with clinical parameters in infertile semen samples. MATERIALS AND METHODS: Semen sample of fifty infertile men were divided into three groups: (1) Group I - Normospermic (count >15 million/ml), Group II - Asthenospermic (motility <32%), and Group III - Oligospermic (counts <15 million/ml) subjects based on the sperm count and sperm motility. The samples were also divided into two groups: (1) Group IV with semen pH >7.2 (25 samples) and Group V - Semen pH <7.2 (25 samples). The grouping was based on the WHO guideline for semen analysis (12). The semen antioxidant parameters like glucose-6-phosphate dehydrogenase (G-6-PDH) (spectrophotometric method Kornberg and Horecker, 1955). Catalase (Maehly and Chance 1954), glutathione peroxidase (GPX) (Rotruck method), glutathione (GSH) (dithiobisnitro-benzoate method), superoxide dismutase (SOD) (direct method), and malondialdehyde (MDA) (thiobarbituric acid reactive substances assay kit method) were investigated. Mann–Whitney U-test was applied to compare the findings. RESULTS: Of fifty semen samples there were 12 normospermic (sperm concentration ≥15 × 10(6)/ml of ejaculates), 24 asthenospermic (sperm motility ≤32%), and 14 oligospermic (sperm concentration ≤15 × 10(6)/ml of ejaculates) subjects. Results suggested that all asthenospermic males were found to have reduced motility and viability when compared with normospermic and oligospermic subjects. Activity of antioxidant parameters such as G-6-PDH, GPX, GSH, and SOD was decreased in case of asthenospermic subjects. The concentration of MDA was increased significantly (P < 0.001) in semen of asthenospermic subjects compared to normospermic and oligospermic subjects. CONCLUSION: The current study concludes that there is a significant relationship of ROS and semen parameters. Further studies will be needed in such subjects regarding role of effectiveness of dietary antioxidants in improving semen qualities.
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spelling pubmed-57879582018-02-07 Relationship of antioxidant system and reactive oxygen species with clinical semen parameters in infertile men Tomar, Gajendra Joshi, Tulika Varghes, Alex Sasidharan, Suresh Kural, Moolraj R. J Family Med Prim Care Original Article AIMS AND OBJECTIVES: To determine the correlation of antioxidant system and reactive oxygen species with clinical parameters in infertile semen samples. MATERIALS AND METHODS: Semen sample of fifty infertile men were divided into three groups: (1) Group I - Normospermic (count >15 million/ml), Group II - Asthenospermic (motility <32%), and Group III - Oligospermic (counts <15 million/ml) subjects based on the sperm count and sperm motility. The samples were also divided into two groups: (1) Group IV with semen pH >7.2 (25 samples) and Group V - Semen pH <7.2 (25 samples). The grouping was based on the WHO guideline for semen analysis (12). The semen antioxidant parameters like glucose-6-phosphate dehydrogenase (G-6-PDH) (spectrophotometric method Kornberg and Horecker, 1955). Catalase (Maehly and Chance 1954), glutathione peroxidase (GPX) (Rotruck method), glutathione (GSH) (dithiobisnitro-benzoate method), superoxide dismutase (SOD) (direct method), and malondialdehyde (MDA) (thiobarbituric acid reactive substances assay kit method) were investigated. Mann–Whitney U-test was applied to compare the findings. RESULTS: Of fifty semen samples there were 12 normospermic (sperm concentration ≥15 × 10(6)/ml of ejaculates), 24 asthenospermic (sperm motility ≤32%), and 14 oligospermic (sperm concentration ≤15 × 10(6)/ml of ejaculates) subjects. Results suggested that all asthenospermic males were found to have reduced motility and viability when compared with normospermic and oligospermic subjects. Activity of antioxidant parameters such as G-6-PDH, GPX, GSH, and SOD was decreased in case of asthenospermic subjects. The concentration of MDA was increased significantly (P < 0.001) in semen of asthenospermic subjects compared to normospermic and oligospermic subjects. CONCLUSION: The current study concludes that there is a significant relationship of ROS and semen parameters. Further studies will be needed in such subjects regarding role of effectiveness of dietary antioxidants in improving semen qualities. Medknow Publications & Media Pvt Ltd 2017 /pmc/articles/PMC5787958/ /pubmed/29417011 http://dx.doi.org/10.4103/2249-4863.222051 Text en Copyright: © 2017 Journal of Family Medicine and Primary Care http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Tomar, Gajendra
Joshi, Tulika
Varghes, Alex
Sasidharan, Suresh
Kural, Moolraj R.
Relationship of antioxidant system and reactive oxygen species with clinical semen parameters in infertile men
title Relationship of antioxidant system and reactive oxygen species with clinical semen parameters in infertile men
title_full Relationship of antioxidant system and reactive oxygen species with clinical semen parameters in infertile men
title_fullStr Relationship of antioxidant system and reactive oxygen species with clinical semen parameters in infertile men
title_full_unstemmed Relationship of antioxidant system and reactive oxygen species with clinical semen parameters in infertile men
title_short Relationship of antioxidant system and reactive oxygen species with clinical semen parameters in infertile men
title_sort relationship of antioxidant system and reactive oxygen species with clinical semen parameters in infertile men
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5787958/
https://www.ncbi.nlm.nih.gov/pubmed/29417011
http://dx.doi.org/10.4103/2249-4863.222051
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