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Antioxidant and hepatoprotective effects of Artemisia dracunculus against CCl(4)-induced hepatotoxicity in rats

OBJECTIVE: The present study was conducted to investigate the antioxidant and hepatoprotective activity of the hydro-alcoholic extract of aerial parts of Artemisia dracunculus (HAAD) against CCl(4)-induced hepatotoxicity in rats. MATERIALS AND METHODS: The antioxidant activity was evaluated by reduc...

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Autores principales: Zarezade, Vahid, Moludi, Jalal, Mostafazadeh, Mostafa, Mohammadi, Mohammad, Veisi, Ali
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5787997/
https://www.ncbi.nlm.nih.gov/pubmed/29387574
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author Zarezade, Vahid
Moludi, Jalal
Mostafazadeh, Mostafa
Mohammadi, Mohammad
Veisi, Ali
author_facet Zarezade, Vahid
Moludi, Jalal
Mostafazadeh, Mostafa
Mohammadi, Mohammad
Veisi, Ali
author_sort Zarezade, Vahid
collection PubMed
description OBJECTIVE: The present study was conducted to investigate the antioxidant and hepatoprotective activity of the hydro-alcoholic extract of aerial parts of Artemisia dracunculus (HAAD) against CCl(4)-induced hepatotoxicity in rats. MATERIALS AND METHODS: The antioxidant activity was evaluated by reducing power, 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and 2, 20-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays. Rats were pre-treated with either 50, 100, and 200 mg/kg of HAAD or silymarin (100 mg/kg; served as the positive control group) for 15 days and they received a single dose of CCl(4) on the last day. Hepatoprotective effects were investigated by assessment of serum biochemical enzymes such as alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), total protein (TP), total bilirubin (TB), malondialdehyde (MDA), and antioxidant enzymes (SOD, CAT, GST and GSH), along with histopathological studies. RESULTS: Total phenolic content was 197.22±3.73 mg gallic acid equivalent/g HAAD dry weight. HAAD indicated powerful activity in FRAP, DPPH and ABTS tests. Acute toxicity study showed that the extract had an LD(50) of >5000 mg/kg. Oral treatment with HAAD exhibited a significant decrease in the levels of AST, ALT, ALP and TB and an increase in the level of TP. The extract significantly diminished MDA levels. The activities of the antioxidant enzymes were significantly augmented in rats pretreated with HAAD 200 mg/kg. Histopathological examination demonstrated lower liver damage in HAAD-treated groups as compared to CCl(4) groups. CONCLUSION: Our findings indicated hepatoprotective effects of the hydro-alcoholic extract of A. dracunculus on CCl(4)-induced hepatic damage in rats and suggested that these effects may be produced by reducing oxidative stress.
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spelling pubmed-57879972018-01-31 Antioxidant and hepatoprotective effects of Artemisia dracunculus against CCl(4)-induced hepatotoxicity in rats Zarezade, Vahid Moludi, Jalal Mostafazadeh, Mostafa Mohammadi, Mohammad Veisi, Ali Avicenna J Phytomed Original Article OBJECTIVE: The present study was conducted to investigate the antioxidant and hepatoprotective activity of the hydro-alcoholic extract of aerial parts of Artemisia dracunculus (HAAD) against CCl(4)-induced hepatotoxicity in rats. MATERIALS AND METHODS: The antioxidant activity was evaluated by reducing power, 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and 2, 20-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays. Rats were pre-treated with either 50, 100, and 200 mg/kg of HAAD or silymarin (100 mg/kg; served as the positive control group) for 15 days and they received a single dose of CCl(4) on the last day. Hepatoprotective effects were investigated by assessment of serum biochemical enzymes such as alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), total protein (TP), total bilirubin (TB), malondialdehyde (MDA), and antioxidant enzymes (SOD, CAT, GST and GSH), along with histopathological studies. RESULTS: Total phenolic content was 197.22±3.73 mg gallic acid equivalent/g HAAD dry weight. HAAD indicated powerful activity in FRAP, DPPH and ABTS tests. Acute toxicity study showed that the extract had an LD(50) of >5000 mg/kg. Oral treatment with HAAD exhibited a significant decrease in the levels of AST, ALT, ALP and TB and an increase in the level of TP. The extract significantly diminished MDA levels. The activities of the antioxidant enzymes were significantly augmented in rats pretreated with HAAD 200 mg/kg. Histopathological examination demonstrated lower liver damage in HAAD-treated groups as compared to CCl(4) groups. CONCLUSION: Our findings indicated hepatoprotective effects of the hydro-alcoholic extract of A. dracunculus on CCl(4)-induced hepatic damage in rats and suggested that these effects may be produced by reducing oxidative stress. Mashhad University of Medical Sciences 2018 /pmc/articles/PMC5787997/ /pubmed/29387574 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Zarezade, Vahid
Moludi, Jalal
Mostafazadeh, Mostafa
Mohammadi, Mohammad
Veisi, Ali
Antioxidant and hepatoprotective effects of Artemisia dracunculus against CCl(4)-induced hepatotoxicity in rats
title Antioxidant and hepatoprotective effects of Artemisia dracunculus against CCl(4)-induced hepatotoxicity in rats
title_full Antioxidant and hepatoprotective effects of Artemisia dracunculus against CCl(4)-induced hepatotoxicity in rats
title_fullStr Antioxidant and hepatoprotective effects of Artemisia dracunculus against CCl(4)-induced hepatotoxicity in rats
title_full_unstemmed Antioxidant and hepatoprotective effects of Artemisia dracunculus against CCl(4)-induced hepatotoxicity in rats
title_short Antioxidant and hepatoprotective effects of Artemisia dracunculus against CCl(4)-induced hepatotoxicity in rats
title_sort antioxidant and hepatoprotective effects of artemisia dracunculus against ccl(4)-induced hepatotoxicity in rats
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5787997/
https://www.ncbi.nlm.nih.gov/pubmed/29387574
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