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Highly multiplexed single-cell quantitative PCR

We present a microfluidic device for rapid gene expression profiling in single cells using multiplexed quantitative polymerase chain reaction (qPCR). This device integrates all processing steps, including cell isolation and lysis, complementary DNA synthesis, pre-amplification, sample splitting, and...

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Detalles Bibliográficos
Autores principales:	VanInsberghe, Michael, Zahn, Hans, White, Adam K., Petriv, Oleh I., Hansen, Carl L.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2018
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5788347/ https://www.ncbi.nlm.nih.gov/pubmed/29377915 http://dx.doi.org/10.1371/journal.pone.0191601

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