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Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen
Meiosis is a specialized cell division that generates gametes, such as eggs and sperm. Errors in meiosis result in miscarriages and are the leading cause of birth defects; however, the molecular origins of these defects remain unknown. Studies in model organisms are beginning to identify the genes a...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Genetics Society of America
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5788524/ https://www.ncbi.nlm.nih.gov/pubmed/29259000 http://dx.doi.org/10.1534/genetics.117.300527 |
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author | Blyth, Julie Makrantoni, Vasso Barton, Rachael E. Spanos, Christos Rappsilber, Juri Marston, Adele L. |
author_facet | Blyth, Julie Makrantoni, Vasso Barton, Rachael E. Spanos, Christos Rappsilber, Juri Marston, Adele L. |
author_sort | Blyth, Julie |
collection | PubMed |
description | Meiosis is a specialized cell division that generates gametes, such as eggs and sperm. Errors in meiosis result in miscarriages and are the leading cause of birth defects; however, the molecular origins of these defects remain unknown. Studies in model organisms are beginning to identify the genes and pathways important for meiosis, but the parts list is still poorly defined. Here we present a comprehensive catalog of genes important for meiosis in the fission yeast, Schizosaccharomyces pombe. Our genome-wide functional screen surveyed all nonessential genes for roles in chromosome segregation and spore formation. Novel genes important at distinct stages of the meiotic chromosome segregation and differentiation program were identified. Preliminary characterization implicated three of these genes in centrosome/spindle pole body, centromere, and cohesion function. Our findings represent a near-complete parts list of genes important for meiosis in fission yeast, providing a valuable resource to advance our molecular understanding of meiosis. |
format | Online Article Text |
id | pubmed-5788524 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Genetics Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-57885242018-01-30 Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen Blyth, Julie Makrantoni, Vasso Barton, Rachael E. Spanos, Christos Rappsilber, Juri Marston, Adele L. Genetics Investigations Meiosis is a specialized cell division that generates gametes, such as eggs and sperm. Errors in meiosis result in miscarriages and are the leading cause of birth defects; however, the molecular origins of these defects remain unknown. Studies in model organisms are beginning to identify the genes and pathways important for meiosis, but the parts list is still poorly defined. Here we present a comprehensive catalog of genes important for meiosis in the fission yeast, Schizosaccharomyces pombe. Our genome-wide functional screen surveyed all nonessential genes for roles in chromosome segregation and spore formation. Novel genes important at distinct stages of the meiotic chromosome segregation and differentiation program were identified. Preliminary characterization implicated three of these genes in centrosome/spindle pole body, centromere, and cohesion function. Our findings represent a near-complete parts list of genes important for meiosis in fission yeast, providing a valuable resource to advance our molecular understanding of meiosis. Genetics Society of America 2018-02 2017-12-19 /pmc/articles/PMC5788524/ /pubmed/29259000 http://dx.doi.org/10.1534/genetics.117.300527 Text en Copyright © 2018 Blyth et al. Available freely online through the author-supported open access option. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Investigations Blyth, Julie Makrantoni, Vasso Barton, Rachael E. Spanos, Christos Rappsilber, Juri Marston, Adele L. Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen |
title | Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen |
title_full | Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen |
title_fullStr | Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen |
title_full_unstemmed | Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen |
title_short | Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen |
title_sort | genes important for schizosaccharomyces pombe meiosis identified through a functional genomics screen |
topic | Investigations |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5788524/ https://www.ncbi.nlm.nih.gov/pubmed/29259000 http://dx.doi.org/10.1534/genetics.117.300527 |
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