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Cooperative Control of Ecdysone Biosynthesis in Drosophila by Transcription Factors Séance, Ouija Board, and Molting Defective
Ecdysteroids are steroid hormones that control many aspects of development and physiology. During larval development, ecdysone is synthesized in an endocrine organ called the prothoracic gland through a series of ecdysteroidogenic enzymes encoded by the Halloween genes. The expression of the Hallowe...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Genetics Society of America
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5788525/ https://www.ncbi.nlm.nih.gov/pubmed/29187506 http://dx.doi.org/10.1534/genetics.117.300268 |
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author | Uryu, Outa Ou, Qiuxiang Komura-Kawa, Tatsuya Kamiyama, Takumi Iga, Masatoshi Syrzycka, Monika Hirota, Keiko Kataoka, Hiroshi Honda, Barry M. King-Jones, Kirst Niwa, Ryusuke |
author_facet | Uryu, Outa Ou, Qiuxiang Komura-Kawa, Tatsuya Kamiyama, Takumi Iga, Masatoshi Syrzycka, Monika Hirota, Keiko Kataoka, Hiroshi Honda, Barry M. King-Jones, Kirst Niwa, Ryusuke |
author_sort | Uryu, Outa |
collection | PubMed |
description | Ecdysteroids are steroid hormones that control many aspects of development and physiology. During larval development, ecdysone is synthesized in an endocrine organ called the prothoracic gland through a series of ecdysteroidogenic enzymes encoded by the Halloween genes. The expression of the Halloween genes is highly restricted and dynamic, indicating that their spatiotemporal regulation is mediated by their tight transcriptional control. In this study, we report that three zinc finger-associated domain (ZAD)-C(2)H(2) zinc finger transcription factors—Séance (Séan), Ouija board (Ouib), and Molting defective (Mld)—cooperatively control ecdysone biosynthesis in the fruit fly Drosophila melanogaster. Séan and Ouib act in cooperation with Mld to positively regulate the transcription of neverland and spookier, respectively, two Halloween genes. Remarkably, loss-of-function mutations in séan, ouib, or mld can be rescued by the expression of neverland, spookier, or both, respectively. These results suggest that the three transcription factors have distinct roles in coordinating the expression of just two genes in Drosophila. Given that neverland and spookier are located in constitutive heterochromatin, Séan, Ouib, and Mld represent the first example of a transcription factor subset that regulates genes located in constitutive heterochromatin. |
format | Online Article Text |
id | pubmed-5788525 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Genetics Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-57885252018-01-30 Cooperative Control of Ecdysone Biosynthesis in Drosophila by Transcription Factors Séance, Ouija Board, and Molting Defective Uryu, Outa Ou, Qiuxiang Komura-Kawa, Tatsuya Kamiyama, Takumi Iga, Masatoshi Syrzycka, Monika Hirota, Keiko Kataoka, Hiroshi Honda, Barry M. King-Jones, Kirst Niwa, Ryusuke Genetics Investigations Ecdysteroids are steroid hormones that control many aspects of development and physiology. During larval development, ecdysone is synthesized in an endocrine organ called the prothoracic gland through a series of ecdysteroidogenic enzymes encoded by the Halloween genes. The expression of the Halloween genes is highly restricted and dynamic, indicating that their spatiotemporal regulation is mediated by their tight transcriptional control. In this study, we report that three zinc finger-associated domain (ZAD)-C(2)H(2) zinc finger transcription factors—Séance (Séan), Ouija board (Ouib), and Molting defective (Mld)—cooperatively control ecdysone biosynthesis in the fruit fly Drosophila melanogaster. Séan and Ouib act in cooperation with Mld to positively regulate the transcription of neverland and spookier, respectively, two Halloween genes. Remarkably, loss-of-function mutations in séan, ouib, or mld can be rescued by the expression of neverland, spookier, or both, respectively. These results suggest that the three transcription factors have distinct roles in coordinating the expression of just two genes in Drosophila. Given that neverland and spookier are located in constitutive heterochromatin, Séan, Ouib, and Mld represent the first example of a transcription factor subset that regulates genes located in constitutive heterochromatin. Genetics Society of America 2018-02 2017-11-29 /pmc/articles/PMC5788525/ /pubmed/29187506 http://dx.doi.org/10.1534/genetics.117.300268 Text en Copyright © 2018 by the Genetics Society of America Available freely online through the author-supported open access option. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Investigations Uryu, Outa Ou, Qiuxiang Komura-Kawa, Tatsuya Kamiyama, Takumi Iga, Masatoshi Syrzycka, Monika Hirota, Keiko Kataoka, Hiroshi Honda, Barry M. King-Jones, Kirst Niwa, Ryusuke Cooperative Control of Ecdysone Biosynthesis in Drosophila by Transcription Factors Séance, Ouija Board, and Molting Defective |
title | Cooperative Control of Ecdysone Biosynthesis in Drosophila by Transcription Factors Séance, Ouija Board, and Molting Defective |
title_full | Cooperative Control of Ecdysone Biosynthesis in Drosophila by Transcription Factors Séance, Ouija Board, and Molting Defective |
title_fullStr | Cooperative Control of Ecdysone Biosynthesis in Drosophila by Transcription Factors Séance, Ouija Board, and Molting Defective |
title_full_unstemmed | Cooperative Control of Ecdysone Biosynthesis in Drosophila by Transcription Factors Séance, Ouija Board, and Molting Defective |
title_short | Cooperative Control of Ecdysone Biosynthesis in Drosophila by Transcription Factors Séance, Ouija Board, and Molting Defective |
title_sort | cooperative control of ecdysone biosynthesis in drosophila by transcription factors séance, ouija board, and molting defective |
topic | Investigations |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5788525/ https://www.ncbi.nlm.nih.gov/pubmed/29187506 http://dx.doi.org/10.1534/genetics.117.300268 |
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